LOCUS Exported 4071 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC41A3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4071) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4071) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4071 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2196..2295) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2312..2331) /label=T7 /note="T7 promoter, forward primer" promoter complement(2313..2331) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2336..2352) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2336..2352) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2454..2473) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2465..3274 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2532..2551) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3421..4009 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3910..3929 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggacg gcaccgagac aaggcagcgg agactggatt cctgcggcaa 721 gcctggagag ctgggactgc ctcacccact gtctaccgga ggactgcctg tggcaagcga 781 ggacggcgcc ctgagggcac cagagtctca gagcgtgacc cccaagcctc tggagacaga 841 gccaagccgc gagaccacat ggtccatcgg cctgcaggtg accgtgccct tcatgtttgc 901 aggactggga ctgagctggg caggaatgct gctggactac tttcagcact ggcccgtgtt 961 cgtggaggtg aaggatctgc tgaccctggt gccacctctg gtgggcctga agggcaacct 1021 ggagatgaca ctggcaagca ggctgtccac cgcagcaaat acaggacaga tcgacgatcc 1081 tcaggagcag cacagagtga tcagctccaa cctggccctg atccaggtgc aggccaccgt 1141 ggtgggactg ctggcagcag tggccgccct gctgctgggc gtggtgtcca gggaggaggt 1201 ggacgtggcc aaggtggagc tgctgtgcgc ctctagcgtg ctgacagcct ttctggcagc 1261 cttcgccctg ggcgtgctga tggtgtgcat cgtgatcgga gcaaggaagc tgggagtgaa 1321 cccagacaat atcgcaaccc ctatcgcagc atccctgggc gatctgatca cactgtctat 1381 cctggccctg gtgtcctctt tcttttaccg gcacaaggat agcagatatc tgaccccact 1441 ggtgtgcctg tccttcgccg ccctgacacc cgtgtgggtg ctgatcgcca agcagtcccc 1501 acccatcgtg aagatcctga agtttggctg gttccctatc atcctggcca tggtcatcag 1561 ctcctttggc ggcctgatcc tgagcaagac cgtgtccaag cagcagtaca agggcatggc 1621 catcttcaca ccagtgatct gtggcgtggg cggcaatctg gtggccatcc agacctctcg 1681 gatcagcaca tatctgcaca tgtggagcgc ccctggcgtg ctgccactgc agatgaagaa 1741 gttttggcca aacccctgct ccaccttctg tacatctgag atcaattcca tgtctgccag 1801 agtgctgctg ctgctggtgg tgccaggcca cctgatcttc ttttacatca tctatctggt 1861 ggagggccag tctgtgatca acagccagac ctttgtggtg ctgtacctgc tggccggcct 1921 gatccaggtg accatcctgc tgtatctggc cgaagtgatg gtgcggctga catggcacca 1981 ggccctggac ccagataatc actgcatccc ctacctgacc ggactgggcg atctgctggg 2041 atctagctcc gtgggacaca ccgcagcagt gcctaggcgc tgcacagcat ctccaggatg 2101 gggactgatc cagcccttca tctgtaccca gcacctgatc gtgtccctgc tgtctttcta 2161 ttttcccttc tgtctgctgg ccaagacaag catcgaccca gctttcttgt acaaagttgg 2221 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa 2281 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat 2341 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga 2401 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 2461 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc 2521 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta 2581 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt 2641 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct 2701 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat 2761 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt 2821 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt 2881 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt 2941 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga 3001 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact 3061 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg 3121 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc 3181 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt 3241 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca 3301 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa 3361 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 3481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 3541 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 3601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 3841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 4021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t //