LOCUS Exported 3471 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC39A9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3471) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3471) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3471 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1596..1695) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1712..1731) /label=T7 /note="T7 promoter, forward primer" promoter complement(1713..1731) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1736..1752) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1736..1752) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1854..1873) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1865..2674 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1932..1951) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2821..3409 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3310..3329 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggacg atttcatctc catctctctg ctgtccctgg caatgctggt 721 gggatgctac gtggcaggca tcatcccact ggccgtgaac ttttctgagg agaggctgaa 781 gctggtgaca gtgctgggag caggactgct gtgcggaacc gcccttgccg ttatagttcc 841 tgaaggggtg cacgccctgt atgaggacat cctggagggc aagcaccacc aggcctccga 901 gacacacaat gtgatcgcct ccgacaaggc cgccgagaag tctgtggtgc acgagcacga 961 gcactctcac gatcacaccc agctgcacgc ctacatcggc gtgagcctgg tgctgggctt 1021 cgtgtttatg ctgctggtgg accagatcgg caactctcac gtgcacagca cagacgatcc 1081 agaggcagca cggagctcca atagcaagat caccacaacc ctgggcctgg tggtgcacgc 1141 agcagcagat ggagtggccc tgggagccgc cgccagcaca tcccagacca gcgtgcagct 1201 gatcgtgttc gtggccatca tgctgcacaa ggcacctgca gccttcggcc tggtgtcctt 1261 tctgatgcac gccggcctgg agcggaacag aatcaggaag cacctgctgg tgtttgcact 1321 ggcagcacca gtgatgagca tggtgacata cctgggcctg tccaagtcta gcaaggaggc 1381 cctgtctgaa gtgaatgcca ccggcgtggc catgctgttc tctgccggca catttctgta 1441 tgtggcaacc gtgcacgtgc tgccagaagt gggaggaatc ggacacagcc acaagcctga 1501 tgcaaccgga ggaaggggac tgtccaggct ggaggtggcc gccctggtgc tgggctgcct 1561 gatccctctg atcctgagcg tgggccacca gcacgaccca gctttcttgt acaaagttgg 1621 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa 1681 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat 1741 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga 1801 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 1861 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc 1921 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta 1981 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt 2041 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct 2101 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat 2161 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt 2221 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt 2281 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt 2341 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga 2401 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact 2461 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg 2521 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc 2581 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt 2641 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca 2701 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa 2761 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 2821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 2881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 2941 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 3001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 3241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t //