LOCUS Exported 3975 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC46A2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3975) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3975) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3975 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2100..2199) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2216..2235) /label=T7 /note="T7 promoter, forward primer" promoter complement(2217..2235) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2240..2256) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2240..2256) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2358..2377) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2369..3178 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2436..2455) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3325..3913 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3814..3833 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgtccc cagaggtgac atgcccaagg agaggacacc tgccaaggtt 721 ccaccctaga acctgggtgg agcctgtggt ggcaagctcc caggtggcag caagcctgta 781 cgacgcagga ctgctgctgg tggtgaaggc atcctatgga accggaggat ctagcaacca 841 ctccgcctct ccaagcccaa ggggcgccct ggaggatcag cagcagcgcg ccatcagcaa 901 cttctacatc atctataatc tggtggtggg actgagccca ctgctgtccg cctacggact 961 gggatggctg tctgaccggt atcacagaaa gatctctatc tgcatgagcc tgctgggctt 1021 tctgctgagc aggctgggcc tgctgctgaa ggtgctgctg gattggcctg tggaggtgct 1081 gtacggagca gccgccctga atggactgtt cggcggcttt tccgccttct ggtctggcgt 1141 gatggcactg ggatctctgg gatcctctga gggcaggcgc agcgtgcgcc tgatcctgat 1201 cgacctgatg ctgggcctgg ccggcttttg tggcagcatg gcctccggcc acctgttcaa 1261 gcagatggca ggacacagcg gacagggact gatcctgaca gcctgctccg tgtcttgtgc 1321 ctctttcgcc ctgctgtaca gcctgctggt gctgaaggtg cctgagagcg tggccaagcc 1381 ttcccaggag ctgccagcag tggacaccgt gtccggaacc gtgggaacat accggaccct 1441 ggacccagat cagctggatc agcagtatgc agtgggacac ccaccttctc caggcaaggc 1501 caagcctcac aagaccacaa tcgccctgct gtttgtgggc gccatcatct atgacctggc 1561 cgtggtgggc acagtggatg tgatcccact gttcgtgctg agggagccac tgggatggaa 1621 ccaggtgcaa gtgggatacg gaatggcagc aggctataca atctttatca cctccttcct 1681 gggcgtgctg gtgttttcta ggtgcttccg cgataccaca atgatcatga tcggcatggt 1741 gtcttttggc agcggcgccc tgctgctggc ctttgtgaag gagacctaca tgttctatat 1801 cgcccgggcc gtgatgctgt tcgccctgat cccagtgacc acaatcagat ccgccatgtc 1861 taagctgatc aagggcagct cctacggcaa ggtgtttgtg atcctgcagc tgtccctggc 1921 cctgacaggc gtggtgacct ctacactgta caataagatc tatcagctga ccatggacat 1981 gtttgtgggc tcctgtttcg ccctgtctag ctttctgagc ttcctggcca tcatccccat 2041 ctccatcgtg gcctacaagc aggtgcctct gagcccatat ggcgatatca tcgagaagga 2101 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa 2161 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta 2221 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa 2281 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 2341 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc 2401 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt 2461 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt 2521 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa 2581 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga 2641 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata 2701 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc 2761 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca 2821 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg 2881 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt 2941 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg 3001 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg 3061 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat 3121 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc 3181 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc 3241 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc 3301 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 3361 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 3421 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 3481 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 3541 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 3601 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 3661 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 3721 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 3781 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 3841 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 3901 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 3961 ttttgctcac atgtt //