LOCUS Exported 4290 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC5A7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4290) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4290) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4290 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2415..2514) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2531..2550) /label=T7 /note="T7 promoter, forward primer" promoter complement(2532..2550) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2555..2571) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2555..2571) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2673..2692) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2684..3493 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2751..2770) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3640..4228 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4129..4148 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggcct tccacgtgga gggcctgatc gccatcatcg tgttttacct 721 gctgatcctg ctggtgggaa tctgggcagc atggaggaca aagaacagcg gatccgccga 781 ggagaggagc gaggcaatca tcgtgggagg aagggacatc ggactgctgg tgggcggctt 841 caccatgaca gcaacctggg tgggaggagg ctacatcaat ggaaccgcag aggccgtgta 901 cgtgccagga tatggactgg catgggcaca ggcacctatc ggatattctc tgagcctgat 961 cctgggcggc ctgttctttg ccaagccaat gcggtccaag ggctacgtga ccatgctgga 1021 tcccttccag cagatctatg gcaagagaat gggcggcctg ctgtttatcc ctgccctgat 1081 gggcgagatg ttctgggcag cagcaatctt tagcgccctg ggagccacaa tctccgtgat 1141 catcgacgtg gatatgcaca tctccgtgat catctctgcc ctgatcgcca cactgtacac 1201 cctggtggga ggactgtaca gcgtggccta taccgacgtg gtgcagctgt tctgcatctt 1261 tgtgggcctg tggatctctg tgcccttcgc cctgagccac cctgcagtgg ccgatatcgg 1321 ctttacagcc gtgcacgcca agtaccagaa gccatggctg ggaaccgtgg acagctccga 1381 ggtgtatagc tggctggatt ccttcctgct gctgatgctg ggcggcatcc cttggcaggc 1441 ctactttcag cgggtgctgt ctagctcctc tgccacctat gcccaggtgc tgtctttcct 1501 ggccgccttt ggctgcctgg tcatggccat cccagcaatc ctgatcggag caatcggagc 1561 cagcacagac tggaaccaga ccgcctacgg cctgccagac cccaagacca cagaggaggc 1621 cgatatgatc ctgcctatcg tgctgcagta cctgtgccca gtgtatatct ctttctttgg 1681 actgggagcc gtgagcgccg ccgtgatgag ctccgccgac tctagcatcc tgagcgcctc 1741 ctctatgttc gcccggaaca tctaccagct gtcctttaga cagaatgcct ctgataagga 1801 gatcgtgtgg gtcatgcgga tcacagtgtt cgtgtttgga gcatccgcca ccgcaatggc 1861 actgctgaca aagaccgtgt acggcctgtg gtatctgagc tccgatctgg tgtatatcgt 1921 gatcttccca cagctgctgt gcgtgctgtt tgtgaagggc acaaatacct acggagcagt 1981 ggcaggatac gtgagcggac tgttcctgag aatcacaggc ggcgagccat acctgtatct 2041 gcagcccctg atcttttacc ctggctacta tccagacgat aacggcatct ataatcagaa 2101 gttccccttt aagaccctgg ccatggtgac atccttcctg accaacatct gtatctctta 2161 cctggccaag tatctgttcg agagcggcac actgccccct aagctggacg tgtttgatgc 2221 cgtggtggcc aggcactccg aggagaatat ggacaagacc atcctggtga agaacgagaa 2281 tatcaagctg gatgagctgg ccctggtgaa gcctcgccag tccatgacac tgtctagcac 2341 attcaccaac aaggaggcct ttctggacgt ggattcctct ccagagggct ctggcaccga 2401 ggacaatctg caggacccag ctttcttgta caaagttggc attataagaa agcattgctt 2461 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc 2521 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg 2581 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac 2641 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg 2701 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg 2761 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga 2821 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga 2881 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat 2941 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca 3001 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct 3061 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg 3121 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga 3181 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt 3241 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga 3301 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga 3361 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt 3421 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga 3481 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac 3541 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca 3601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 3661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 3721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 3781 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 3841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 3901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 4021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 4081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 4141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 4201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 4261 ggccttttgc tggccttttg ctcacatgtt //