LOCUS Exported 11989 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant). ACCESSION . VERSION . KEYWORDS pCEP4-myc-HLA-A2-VN (L156P) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11989) AUTHORS McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG TITLE Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection. JOURNAL Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 10.1073/pnas.1915562116. Epub 2019 Dec 3. PUBMED 31796585 REFERENCE 2 (bases 1 to 11989) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11989 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 61..90 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1138..1599 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN155(I152L) /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA" primer_bind complement(1180..1201) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1441..1460) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" polyA_signal 1622..1756 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1646..1665) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1700..1719 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1898..1920) /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 2506..4295 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(4601..6526) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(6961..6979) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7047..7151 /gene="bla" /label=AmpR promoter CDS 7152..8012 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7370..7389) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8183..8771 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8672..8691 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 9388..9533 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 9573..10598 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" primer_bind complement(10619..10638) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 10663..10711 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" enhancer 11364..11743 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 11744..11947 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 11898..11918 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 11944..11968 /label=LNCX /note="Human CMV promoter, forward primer" ORIGIN 1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc 61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcagttc aggaggcgga 121 tctcactcca tgaggtattt cttcacatcc gtgtcccggc ccggccgcgg ggagccccgc 181 ttcatcgcag tgggctacgt ggacgacacg cagttcgtgc ggttcgacag cgacgccgcg 241 agccagagga tggagccgcg ggcgccgtgg atagagcagg agggtccgga gtattgggac 301 ggggagacac ggaaagtgaa ggcccactca cagactcacc gagtggacct ggggaccctg 361 cgcggctact acaaccagag cgaggccggt tctcacaccg tccagaggat gtatggctgc 421 gacgtggggt cggactggcg cttcctccgc gggtaccacc agtacgccta cgacggcaag 481 gattacatcg ccctgaaaga ggacctgcgc tcttggaccg cggcggacat ggcagctcag 541 accaccaagc acaagtggga ggcggcccat gtggcggagc agcctagagc ctacctggag 601 ggcacgtgcg tggagtggct ccgcagatac ctggagaacg ggaaggagac gctgcagcgt 661 acggacgccc ccaaaacgca tatgactcac cacgctgtct ctgaccatga agccaccctg 721 aggtgctggg ccctgagctt ctaccctgcg gagatcacac tgacctggca gcgggatggg 781 gaggaccaga cccaggacac ggagctcgtg gagaccaggc ctgcagggga tggaaccttc 841 cagaagtggg cggctgtggt ggtgccttct ggacaggagc agagatacac ctgccatgtg 901 cagcatgagg gtttgcccaa gcccctcacc ctgagatggg agccgtcttc ccagcccacc 961 atccccatcg tgggcatcat tgctggcctg gttctctttg gagctgtgat cactggagct 1021 gtggtcgctg ctgtgatgtg gaggaggaag agctcagata gaaaaggagg gagctactct 1081 caggctgcaa gcagtgacag tgcccagggc tctgatgtgt ctctcacagc ttgtaaagtg 1141 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 1201 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 1261 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 1321 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac 1381 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 1441 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 1501 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 1561 gagtacaact acaacagcca caacgtctat ctgaccgcct aactcgaggc cggcaaggcc 1621 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 1681 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 1741 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 1801 ggtggggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat 1861 ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 1921 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 1981 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccgg tcgactctag agccccgccg 2041 ccggacgaac taaacctgac tacggcatct ctgccccttc ttcgctggta cgaggagcgc 2101 ttttgttttg tattcggggc agtgcatgta atcccttcag ttggttggta caacttgcca 2161 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact 2221 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct 2281 ggagcagact ttgcatgctg tggactgcaa cacaacattg cctttatgtg taactcttgg 2341 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg 2401 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa 2461 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg 2521 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc 2581 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat 2641 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag 2701 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc 2761 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg 2821 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat 2881 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca 2941 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata 3001 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg 3061 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca 3121 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag 3181 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg 3241 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt 3301 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata 3361 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc 3421 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata 3481 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc 3541 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc 3601 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg 3661 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg 3721 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg 3781 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg 3841 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg 3901 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag 3961 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag 4021 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag 4081 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag 4141 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag 4201 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa 4261 gggggcgtga attttcgctg cttgtccttt tcctgcatgc tggttgctcc cattcttagg 4321 tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc 4381 gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg 4441 gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa 4501 tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac 4561 ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac 4621 cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc 4681 cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag 4741 tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct 4801 tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact 4861 cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca 4921 atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg 4981 aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg tcggtagtcc 5041 tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg 5101 ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg cgcctgcctc 5161 catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct gcggggccct 5221 gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct tggtggtatt 5281 caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga gggggcctgc 5341 gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc ttttctccac 5401 gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt tcacgtcctc 5461 taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct cgaccccggc 5521 ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct gctcctgccc 5581 ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc 5641 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc 5701 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc 5761 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc 5821 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc 5881 ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct cctgctcctg 5941 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc 6001 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc 6061 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg 6121 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc 6181 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc 6241 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac 6301 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg 6361 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac 6421 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct 6481 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa 6541 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg 6601 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg 6661 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg 6721 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat 6781 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc 6841 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc 6901 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg 6961 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 7021 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 7081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 7141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 7201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 7261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 7321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 7381 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc 7441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 7501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 7561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 7621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 7681 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 7741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 7801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 7861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 7921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 7981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 8041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 8101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 8161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 8221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 8281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 8341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 8401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 8461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 8521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 8581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 8641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 8701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 8761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctgcgccg 8821 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc 8881 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc 8941 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac 9001 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg 9061 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg 9121 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac 9181 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg 9241 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc 9301 atgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa 9361 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg 9421 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt 9481 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc 9541 aacagcgtgc cgcagatccc gggcaatgag atatgaaaaa gcctgaactc accgcgacgt 9601 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg 9661 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg 9721 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg 9781 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt 9841 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg 9901 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga 9961 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt 10021 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg 10081 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg 10141 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc 10201 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg 10261 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg 10321 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg 10381 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc 10441 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg 10501 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa 10561 accgacgccc cagcactcgt ccgagggcaa aggaataggg gagatggggg aggctaactg 10621 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 10681 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 10741 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 10801 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 10861 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg 10921 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtctggtc cacgactgga 10981 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg 11041 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg 11101 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga 11161 tccgggcaac gttgttgcat tgctgcaggc gcagaactgg taggtatgga agatctatac 11221 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat 11281 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc 11341 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat 11401 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 11461 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 11521 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 11581 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt 11641 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc 11701 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 11761 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 11821 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa 11881 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 11941 cagagctcgt ttagtgaacc gtcagatctc tagaagctgg gtaccagct //