LOCUS       Exported                5622 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGL2-50bp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Wang S, El-Deiry WS
  TITLE     p73 or p53 directly regulates human p53 transcription to maintain 
            cell cycle checkpoints.
  JOURNAL   Cancer Res. 2006 Jul 15. 66(14):6982-9.
  PUBMED    16849542
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             100..1752
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(166..184)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          1993..2058
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2188..2208
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2633..2767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2909..2926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3080..3668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3160..3179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..4699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4462..4481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4700..4804)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4831..5286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4918..4937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5128..5149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5476..5597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctcc ccatgtgctc aagactggcg ctaaaagttt tgagcttctc
       61 aaaagtctag aagcttggca ttccggtact gttggtaaaa tggaagacgc caaaaacata
      121 aagaaaggcc cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat
      181 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
      241 gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg
      301 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
      361 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
      421 atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt
      481 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag
      541 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
      601 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat
      661 cgtgacaaaa caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt
      721 gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt
      781 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
      841 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
      901 tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta
      961 gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct
     1021 aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt
     1081 gcaaaacgct tccatcttcc agggatacga caaggatatg ggctcactga gactacatca
     1141 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     1201 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga
     1261 ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg
     1321 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     1381 gaagacgaac acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat
     1441 caggtggccc ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg
     1501 ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     1561 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     1621 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     1681 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     1741 tccaaattgt aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta ttgtaatact
     1801 gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc
     1861 ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgccggatc
     1921 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1981 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     2041 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     2101 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     2161 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     2221 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     2281 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     2341 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     2401 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     2461 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     2521 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2581 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2641 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2701 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2761 ctggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     2821 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     2881 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4801 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     4861 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4921 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4981 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5041 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5101 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5161 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5221 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     5281 caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5341 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgtggag
     5401 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     5461 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5581 ctcatcaatg tatcttatgg tactgtaact gagctaacat aa
//
