LOCUS       Exported                9518 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    GABBR1-Tango
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4076..5077
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4143..4162)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(5109..5126)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5115..5339
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5385..5813
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5472..5491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5682..5703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5822..5842)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5827..6156
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6007..6142
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6069..6088
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6223..7017
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6277..6296)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6887..6906
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7191..7312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7228..7247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7282..7301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7361..7377)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7361..7377)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7374..7396)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7385..7401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7409..7439)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7454..7475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7592..7609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7763..8351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7843..7862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8522..9382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9145..9164
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9383..9487)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgcgccac catgaagacg atcatcgccc tgagctacat
     1021 cttctgcctg gtattcgccg actacaagga cgatgatgac gccagcatcg atatgctcct
     1081 cctgcttttg ctcgcaccac tcttcctgcg cccaccaggg gcaggggggg cgcagacacc
     1141 taacgctaca agtgagggct gccaaattat tcatccgcct tgggaggggg ggataagata
     1201 ccgcggcttg acacgggatc aggtcaaggc catcaatttc cttccggtgg attatgagat
     1261 cgaatacgtc tgcagaggcg aaagggaggt ggttggacct aaggtgcgca aatgtcttgc
     1321 caatggaagt tggacggata tggatacacc aagcaggtgt gtcagaattt gtagcaaaag
     1381 ttatctgact cttgagaatg ggaaagtctt tctgaccggc ggagaccttc ccgctctgga
     1441 cggggctcgg gtggatttcc ggtgcgaccc tgactttcac ctcgtaggca gtagccggtc
     1501 aatttgcagc caaggccaat ggtcaacccc taaaccccac tgccaagtga atcgcacacc
     1561 gcactccgag agacgagctg tttacatcgg ggcccttttt cccatgtccg gcgggtggcc
     1621 aggtggacag gcttgtcagc ccgctgttga aatggctctg gaagacgtaa atagccggcg
     1681 ggatatactg cccgattacg aactgaaact cattcatcac gactccaaat gtgatcccgg
     1741 gcaggcaact aaatatctct atgagctctt gtacaacgat cccattaaaa ttattctgat
     1801 gcctggctgc agctctgtga gtaccctcgt ggcagaagcg gcacgaatgt ggaatctgat
     1861 tgttctgagc tacggtagct ccagccccgc tttgtctaat cggcagaggt tccccacctt
     1921 ctttcgcacc cacccttccg ctacccttca taatcctaca cgcgtaaagc tttttgaaaa
     1981 atggggctgg aaaaaaatcg cgaccattca gcagactact gaggtattta ctagcacact
     2041 ggacgatctt gaggagagag tgaaggaagc aggaattgag atcactttcc ggcaaagctt
     2101 ttttagcgac ccggcggtgc ccgtcaagaa ccttaaaagg caagacgccc gaatcatcgt
     2161 gggattgttc tacgaaacgg aggctcggaa agttttttgc gaggtataca aggaacgcct
     2221 gttcggtaaa aagtacgtgt ggttcctcat tgggtggtat gcggataact ggtttaaaat
     2281 atacgacccc tcaataaact gcacagtaga cgagatgacg gaagccgtgg agggccacat
     2341 cacaacagaa attgttatgt tgaaccccgc taacaccaga agcatatcca acatgacttc
     2401 tcaagaattt gttgagaagc ttacaaagcg gctgaagcgc caccctgagg aaacaggggg
     2461 atttcaggag gctcctctcg cctacgacgc catctgggcc ttggccctgg cgttgaataa
     2521 gacatccggc ggggggggcc gatccggggt gaggcttgag gactttaatt ataacaacca
     2581 aacaatcacc gaccagatct acagggcgat gaactctagt agtttcgagg gcgtctccgg
     2641 ccatgtggtc ttcgacgcaa gcgggtcacg gatggcctgg actctgatcg aacagctgca
     2701 gggtggttcc tataaaaaga tagggtacta tgattccaca aaagacgacc tctcctggtc
     2761 caagaccgat aagtggattg gggggagccc acctgctgac cagaccctcg tgattaagac
     2821 tttcaggttc ctctcacaga agctgtttat ttctgtgtct gtcctgtctt ctctgggcat
     2881 cgtgctcgcc gttgtctgtc tgtcctttaa catctacaac tctcacgtca gatatatcca
     2941 gaactcccag cctaacctca ataaccttac cgctgtggga tgctctctcg cattggccgc
     3001 cgtgtttcct ttgggcttgg acggctatca catcggccga aatcagtttc ctttcgtttg
     3061 ccaggctcgg ctgtggttgc tgggactggg attcagtctg ggttatggat ccatgttcac
     3121 taagatctgg tgggttcata ctgtctttac aaaaaaagag gagaagaagg aatggcgcaa
     3181 gacgctcgaa ccgtggaaac tctatgcaac cgtgggcctg ttggtgggaa tggacgtcct
     3241 gactctcgca atctggcaga tagttgatcc cctgcacaga accattgaaa ctttcgccaa
     3301 agaggagccg aaggaggata tcgacgtatc aattctgccc cagttggagc actgcagtag
     3361 caggaagatg aacacatggt tgggcatttt ctacgggtac aagggcctgc ttttgctgct
     3421 cgggattttc ctggcatacg aaaccaagag cgtgtccacc gaaaagatca acgatcacag
     3481 ggcggtgggg atggctatct acaatgtggc cgtcctgtgt ctcatcaccg caccagtgac
     3541 aatgattttg tcctctcagc aggatgcagc cttcgctttc gcatctctgg ccatcgtgtt
     3601 tagcagctac attacactgg tggtactttt tgtaccaaag atgagacgac tgataacgag
     3661 gggagaatgg caaagtgagg cccaggatac catgaaaaca ggctcatcta ctaacaataa
     3721 cgaagaggaa aagtctaggc tcctcgaaaa ggagaatagg gaactggaga aaatcattgc
     3781 agagaaggag gagcgcgtct ctgagctgag gcatcagctg caaagcaggc agcaattgcg
     3841 cagccgccgc catcctccta ccccaccaga gcctagcggg ggacttccac gaggcccccc
     3901 agagccacct gacaggctga gctgcgatgg aagtcgcgtt cacctcctgt ataaaatcga
     3961 taccggtgga cgcaccccac ccagcctggg tccccaagat gagtcctgca ccaccgccag
     4021 ctcctccctg gccaaggaca cttcatcgac cggtgagaac ctgtacttcc agctaagatt
     4081 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
     4141 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
     4201 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
     4261 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
     4321 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
     4381 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
     4441 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
     4501 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
     4561 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
     4621 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga
     4681 aagtgggtcc gcgtacagcc gcgcgcgtac gaaaaacaat tacgggtcta ccatcgaggg
     4741 cctgctcgat ctcccggacg acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct
     4801 gtcctttctc cccgcgggac acacgcgcag actgtcgacg gcccccccga ccgatgtcag
     4861 cctgggggac gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct
     4921 agacgatttc gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc
     4981 ccacgactcc gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt
     5041 taccgatgcc cttggaattg acgagtacgg tgggtagtct agagggcccg tttaaacccg
     5101 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5161 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5221 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5281 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5341 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     5401 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5461 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5521 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5581 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5641 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5701 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5761 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5821 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5881 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5941 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6001 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6061 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     6121 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     6181 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6241 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6301 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6361 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6421 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6481 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6541 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6601 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6661 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6721 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6781 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6841 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6901 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6961 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     7021 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7081 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7141 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7321 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7501 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7561 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7621 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7681 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7741 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7801 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7861 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7921 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7981 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8041 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8101 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8161 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8221 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8281 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8341 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8401 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8461 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8521 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8581 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8641 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8701 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8761 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8821 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8881 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8941 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9001 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9061 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9121 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9181 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9241 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9301 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9361 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9421 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9481 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
