LOCUS       Exported                 859 bp ds-DNA     linear   SYN 01-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3-myc3-CUL4A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Liu J, Furukawa M, Matsumoto T, Xiong Y
  TITLE     NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of 
            CUL1-SKP1 binding and SCF ligases.
  JOURNAL   Mol Cell. 2002 Dec . 10(6):1511-8.
  PUBMED    12504025
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..113
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             156..185
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atggagcaaa agctcatctc agaggaagat ctggaattag
      121 agcaaaagct catctcagag gaagatctgg aattagagca aaagctcatc tcagaggaag
      181 atctggaatt caggatcccc cgggctgcag gaattcggca cgagccagcc atggcggacg
      241 aggccccgcg gaagggcagc ttctcggcgc tcgtgggccg caccaacggc ctcaccaagc
      301 ccgcggccct ggccgccgcg cccgccaagc cggggggcgc gggcggctcc aagaagctgg
      361 tcatcaagaa cttccgagac agacctcggc tgcccgacaa ctacacgcag gacacgtggc
      421 ggaagctgca cgaggcggtg cgggccgtgc agagcagcac ctccatcagg tacaacctcg
      481 aggagctcta ccaggctgtg gaaaatctct gttctcacaa agtctcccca atgctctaca
      541 agcaactgcg tcaggcctgt gaagaccacg tccaggcaca gatccttccg tttagagaag
      601 actcactaga tagtgtttta tttttaaaga agattaacac gtgctggcag gaccactgca
      661 gacaaatgat catgatcaga agcatcttcc tgttcttgga ccgcacctat gtgctgcaga
      721 actccacgct gccctccatc tgggatatgg gattagaact gtttagaacc catattatta
      781 gtgataaaat ggttcagagt aaaaccattg atggaatcct actgctgatc gagcgcgaga
      841 ggagcggcga ggccgtgga
//
