LOCUS       Exported               11205 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Conditionally express both AsCas12a and CRISPR arrays on single 
            transcripts.
ACCESSION   .
VERSION     .
KEYWORDS    pCI108-SiT-Cas12a-[Cond]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ
  TITLE     Multiplexed genome engineering by Cas12a and CRISPR arrays encoded 
            on single transcripts.
  JOURNAL   Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6.
  PUBMED    31406383
REFERENCE   2  (bases 1 to 11205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        292..1470
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          523..1461
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1418..1438
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(1501..1534)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    1701..1835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1955..2089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2209..2343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2372..2405)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             2438..6358
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      6078..6087
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6359..6406
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6413..6493
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(6796..6813)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6802..7026
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7072..7500
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7159..7178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7369..7390
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7509..7529)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7514..7843
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7694..7829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7756..7775
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7910..8704
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7964..7983)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8574..8593
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8878..8999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9048..9064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9048..9064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9061..9083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9072..9088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9096..9126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9141..9162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9279..9296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9450..10038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9530..9549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10209..11069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10832..10851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11070..11174)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga cttcgcgttg acattgatta
      181 ttgactagtc ttaattaacg cgtttaatta atgcaaagat ggataaagtt ttaaacagag
      241 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      301 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      361 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      421 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      481 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      541 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      601 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      661 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      721 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      781 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      841 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      901 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
      961 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     1021 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1081 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1141 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1201 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1261 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1321 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1381 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1441 agtttttttc ttccatttca ggtgtcgtga gctagccgtc gacatttaaa tcatttaaat
     1501 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagcttgca
     1561 gatctgcgac tctagaggat ctgcgactct agaggatcat aatcagccat accacatttg
     1621 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     1681 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1741 atagcatcac aaatttcaca aataaagtat ttttttcact gcattctagt tgtggtttgt
     1801 ccaaactcat caatgtatct tatcatgtct ggatctgcga ctctagagga tcataatcag
     1861 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     1921 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     1981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2041 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gcgactctag
     2101 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2161 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2221 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2281 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     2341 atccccatca agctgatccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     2401 gttattaggt ccctcgacct gcagcggtac cgccaccatg acacagttcg agggctttac
     2461 caacctgtat caggtgagca agacactgcg gtttgagctg atcccacagg gcaagaccct
     2521 gaagcacatc caggagcagg gcttcatcga ggaggacaag gcccgcaatg atcactacaa
     2581 ggagctgaag cccatcatcg atcggatcta caagacctat gccgaccagt gcctgcagct
     2641 ggtgcagctg gattgggaga acctgagcgc cgccatcgac tcctatagaa aggagaaaac
     2701 cgaggagaca aggaacgccc tgatcgagga gcaggccaca tatcgcaatg ccatccacga
     2761 ctacttcatc ggccggacag acaacctgac cgatgccatc aataagagac acgccgagat
     2821 ctacaagggc ctgttcaagg ccgagctgtt taatggcaag gtgctgaagc agctgggcac
     2881 cgtgaccaca accgagcacg agaacgccct gctgcggagc ttcgacaagt ttacaaccta
     2941 cttctccggc ttttatgaga acaggaagaa cgtgttcagc gccgaggata tcagcacagc
     3001 catcccacac cgcatcgtgc aggacaactt ccccaagttt aaggagaatt gtcacatctt
     3061 cacacgcctg atcaccgccg tgcccagcct gcgggagcac tttgagaacg tgaagaaggc
     3121 catcggcatc ttcgtgagca cctccatcga ggaggtgttt tccttccctt tttataacca
     3181 gctgctgaca cagacccaga tcgacctgta taaccagctg ctgggaggaa tctctcggga
     3241 ggcaggcacc gagaagatca agggcctgaa cgaggtgctg aatctggcca tccagaagaa
     3301 tgatgagaca gcccacatca tcgcctccct gccacacaga ttcatccccc tgtttaagca
     3361 gatcctgtcc gataggaaca ccctgtcttt catcctggag gagtttaaga gcgacgagga
     3421 agtgatccag tccttctgca agtacaagac actgctgaga aacgagaacg tgctggagac
     3481 agccgaggcc ctgtttaacg agctgaacag catcgacctg acacacatct tcatcagcca
     3541 caagaagctg gagacaatca gcagcgccct gtgcgaccac tgggatacac tgaggaatgc
     3601 cctgtatgag cggagaatct ccgagctgac aggcaagatc accaagtctg ccaaggagaa
     3661 ggtgcagcgc agcctgaagc acgaggatat caacctgcag gagatcatct ctgccgcagg
     3721 caaggagctg agcgaggcct tcaagcagaa aaccagcgag atcctgtccc acgcacacgc
     3781 cgccctggat cagccactgc ctacaaccct gaagaagcag gaggagaagg agatcctgaa
     3841 gtctcagctg gacagcctgc tgggcctgta ccacctgctg gactggtttg ccgtggatga
     3901 gtccaacgag gtggaccccg agttctctgc ccggctgacc ggcatcaagc tggagatgga
     3961 gccttctctg agcttctaca acaaggccag aaattatgcc accaagaagc cctactccgt
     4021 ggagaagttc aagctgaact ttcagatgcc tacactggcc tctggctggg acgtgaataa
     4081 ggagaagaac aatggcgcca tcctgtttgt gaagaacggc ctgtactatc tgggcatcat
     4141 gccaaagcag aagggcaggt ataaggccct gagcttcgag cccacagaga aaaccagcga
     4201 gggctttgat aagatgtact atgactactt ccctgatgcc gccaagatga tcccaaagtg
     4261 cagcacccag ctgaaggccg tgacagccca ctttcagacc cacacaaccc ccatcctgct
     4321 gtccaacaat ttcatcgagc ctctggagat cacaaaggag atctacgacc tgaacaatcc
     4381 tgagaaggag ccaaagaagt ttcagacagc ctacgccaag aaaaccggcg accagaaggg
     4441 ctacagagag gccctgtgca agtggatcga cttcacaagg gattttctgt ccaagtatac
     4501 caagacaacc tctatcgatc tgtctagcct gcggccatcc tctcagtata aggacctggg
     4561 cgagtactat gccgagctga atcccctgct gtaccacatc agcttccaga gaatcgccga
     4621 gaaggagatc atggatgccg tggagacagg caagctgtac ctgttccaga tctataacaa
     4681 ggactttgcc aagggccacc acggcaagcc taatctgcac acactgtatt ggaccggcct
     4741 gttttctcca gagaacctgg ccaagacaag catcaagctg aatggccagg ccgagctgtt
     4801 ctaccgccct aagtccagga tgaagaggat ggcacaccgg ctgggagaga agatgctgaa
     4861 caagaagctg aaggatcaga aaaccccaat ccccgacacc ctgtaccagg agctgtacga
     4921 ctatgtgaat cacagactgt cccacgacct gtctgatgag gccagggccc tgctgcccaa
     4981 cgtgatcacc aaggaggtgt ctcacgagat catcaaggat aggcgcttta ccagcgacaa
     5041 gttctttttc cacgtgccta tcacactgaa ctatcaggcc gccaattccc catctaagtt
     5101 caaccagagg gtgaatgcct acctgaagga gcaccccgag acacctatca tcggcatcga
     5161 tcggggcgag agaaacctga tctatatcac agtgatcgac tccaccggca agatcctgga
     5221 gcagcggagc ctgaacacca tccagcagtt tgattaccag aagaagctgg acaacaggga
     5281 gaaggagagg gtggcagcaa ggcaggcctg gtctgtggtg ggcacaatca aggatctgaa
     5341 gcagggctat ctgagccagg tcatccacga gatcgtggac ctgatgatcc actaccaggc
     5401 cgtggtggtg ctggagaacc tgaatttcgg ctttaagagc aagaggaccg gcatcgccga
     5461 gaaggccgtg taccagcagt tcgagaagat gctgatcgat aagctgaatt gcctggtgct
     5521 gaaggactat ccagcagaga aagtgggagg cgtgctgaac ccataccagc tgacagacca
     5581 gttcacctcc tttgccaaga tgggcaccca gtctggcttc ctgttttacg tgcctgcccc
     5641 atatacatct aagatcgatc ccctgaccgg cttcgtggac cccttcgtgt ggaaaaccat
     5701 caagaatcac gagagccgca agcacttcct ggagggcttc gactttctgc actacgacgt
     5761 gaaaaccggc gacttcatcc tgcactttaa gatgaacaga aatctgtcct tccagagggg
     5821 cctgcccggc tttatgcctg catgggatat cgtgttcgag aagaacgaga cacagtttga
     5881 cgccaagggc acccctttca tcgccggcaa gagaatcgtg ccagtgatcg agaatcacag
     5941 attcaccggc agataccggg acctgtatcc tgccaacgag ctgatcgccc tgctggagga
     6001 gaagggcatc gtgttcaggg atggctccaa catcctgcca aagctgctgg agaatgacga
     6061 ttctcacgcc atcgacacca tggtggccct gatccgcagc gtgctgcaga tgcggaactc
     6121 caatgccgcc acaggcgagg actatatcaa cagccccgtg cgcgatctga atggcgtgtg
     6181 cttcgactcc cggtttcaga acccagagtg gcccatggac gccgatgcca atggcgccta
     6241 ccacatcgcc ctgaagggcc agctgctgct gaatcacctg aaggagagca aggatctgaa
     6301 gctgcagaac ggcatctcca atcaggactg gctggcctac atccaggagc tgcgcaacaa
     6361 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat cctacccata
     6421 cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc catatgatgt
     6481 ccccgactat gcctaagaat tcgattcgtc agtagggttg taaaggtttt tcttttcctg
     6541 agaaaacaac cttttgtttt ctcaggtttt gctttttggc ctttccctag ctttaaaaaa
     6601 aaaaaagcaa aactcaccga ggcagttcca taggatggca agatcctggt attggtctgc
     6661 gaaatttcta ctcttgtaga tggagacgca aattctaatc cagcttcaac agctatttca
     6721 caagctttca tgatttctcg tctctaattt ctactcttgt agattctaga gggcccgttt
     6781 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6841 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6901 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6961 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7021 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     7081 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7141 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7201 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7261 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7321 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7381 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7441 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7501 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7561 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7621 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7681 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7741 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7801 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7861 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     7921 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     7981 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     8041 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     8101 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     8161 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     8221 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     8281 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     8341 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     8401 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     8461 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     8521 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8581 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     8641 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8701 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     8761 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8821 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9001 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     9061 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9121 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9181 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9241 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
