LOCUS       Exported                 755 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3-myc3-14-3-3 Beta
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Shumway SD, Li Y, Xiong Y
  TITLE     14-3-3beta binds to and negatively regulates the tuberous sclerosis 
            complex 2 (TSC2) tumor suppressor gene product, tuberin.
  JOURNAL   J Biol Chem. 2003 Jan 24. 278(4):2089-92.
  PUBMED    12468542
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             68..97
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             104..133
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             140..169
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agctatggag caaaagctca tctcagagga agatctggaa ttagagcaaa agctcatctc
      121 agaggaagat ctggaattag agcaaaagct catctcagag gaagatctgg aattcaggat
      181 cctgggaatg acaatggata aaagtgagct ggtacagaaa gccaaactcg ctgagcaggc
      241 tgagcgctat gatgatatgg ctgcagccat gaaggcagtc acagaacagg ggcatgaact
      301 ctccaacgaa gagagaaatc tgctctctgt tgcctacaag aatgtggtag gcgcccgccg
      361 ctcttcctgg cgtgtcatct ccagcattga gcagaaaaca gagaggaatg agaagaagca
      421 gcagatgggc aaagagtacc gtgagaagat agaggcagaa ctgcaggaca tctgcaatga
      481 tgttctggag ctgttggaca aatatcttat tcccaatgct acacaaccag aaagtaaggt
      541 gttctacttg aaaatgaaag gagattattt taggtatctt tctgaagtgg catctggaga
      601 caacaaacaa accactgtgt cgaactccca gcaggcttac caggaagcat ttgaaattag
      661 taagaaagaa atgcagccta cacacccaat tcgtcttggt ctggcactaa atttctcagt
      721 cttttactat gagattctaa actctcctga aaagg
//
