LOCUS Exported 4440 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC3A2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4440) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4440) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4440 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2565..2664) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2681..2700) /label=T7 /note="T7 promoter, forward primer" promoter complement(2682..2700) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2705..2721) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2705..2721) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2823..2842) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2834..3643 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2901..2920) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3790..4378 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4279..4298 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggagc tgcagccacc tgaggcatcc atcgcagtgg tgtctatccc 721 caggcagctg cctggcagcc actccgaggc aggagtgcag ggactgagcg ccggcgacga 781 ttccgagctg ggatctcact gcgtggcaca gaccggactg gagctgctgg catccggcga 841 tcctctgcca tctgccagcc agaacgccga gatgatcgag accggaagcg attgcgtgac 901 acaggcagga ctgcagctgc tggcaagctc cgacccaccc gccctggcct ccaagaatgc 961 cgaggtgacc ggcacaatgt ctcaggacac agaggtggat atgaaggagg tggagctgaa 1021 cgagctggag ccagagaagc agccaatgaa tgcagcaagc ggagcagcaa tgtccctggc 1081 aggagcagag aagaacggac tggtgaagat caaggtggca gaggacgagg cagaggcagc 1141 agcagccgcc aagttcaccg gcctgagcaa ggaggagctg ctgaaggtgg caggatcccc 1201 aggatgggtg aggacaagat gggcactgct gctgctgttt tggctgggat ggctgggaat 1261 gctggcagga gcagtggtca tcatcgtgcg ggcacctagg tgcagagagc tgccagcaca 1321 gaagtggtgg cacaccggcg ccctgtacag aatcggcgat ctgcaggcat tccagggaca 1381 cggagcagga aatctggccg gcctgaaggg ccggctggac tatctgtcta gcctgaaggt 1441 gaagggcctg gtgctgggcc ccatccacaa gaaccagaag gacgatgtgg cccagacaga 1501 cctgctgcag atcgatccta atttcggctc taaggaggac tttgatagcc tgctgcagag 1561 cgccaagaag aagagcatca gagtgatcct ggatctgacc cctaactacc gcggcgagaa 1621 tagctggttt tccacacagg tggataccgt ggccacaaag gtgaaggacg cactggagtt 1681 ctggctgcag gcaggagtgg acggatttca ggtgagggat atcgagaacc tgaaggacgc 1741 ctcctctttc ctggccgagt ggcagaatat caccaagggc ttttccgagg atcgcctgct 1801 gatcgccggc acaaacagct ccgacctgca gcagatcctg tctctgctgg agagcaataa 1861 ggatctgctg ctgacctcta gctacctgtc cgactctggc agcaccggcg agcacacaaa 1921 gagcctggtg acccagtatc tgaacgccac aggcaatcgg tggtgttcct ggtctctgag 1981 ccaggccaga ctgctgacat ccttcctgcc tgcccagctg ctgcggctgt accagctgat 2041 gctgttcacc ctgcccggca cacccgtgtt cagctatggc gacgagatcg gactggatgc 2101 agccgccctg ccaggacagc ccatggaggc cccagtgatg ctgtgggatg agtcctcttt 2161 ccctgacatc ccaggcgccg tgagcgccaa catgaccgtg aagggacagt ccgaggaccc 2221 aggatctctg ctgagcctgt ttcggagact gtccgatcag aggtctaagg agcgcagcct 2281 gctgcacggc gacttccacg ccttttccgc cggaccaggc ctgttctctt acatccggca 2341 ctgggatcag aacgagagat tcctggtggt gctgaatttt ggcgatgtgg gactgtctgc 2401 cggactgcag gcaagcgacc tgccagcatc cgcctctctg cctgcaaagg cagacctgct 2461 gctgtctacc cagccaggaa gggaggaggg aagcccactg gagctggagc gcctgaagct 2521 ggagcctcac gagggcctgc tgctgagatt tccatatgcc gccgacccag ctttcttgta 2581 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat 2641 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta 2701 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat 2761 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 2821 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa 2881 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc 2941 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa 3001 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt 3061 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac 3121 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga 3181 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa 3241 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa 3301 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt 3361 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga 3421 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg 3481 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga 3541 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat 3601 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg 3661 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa 3721 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3901 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 4021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 4081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 4141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 4201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 4261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 4321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 4381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt //