LOCUS Exported 4434 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC6A18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4434) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4434) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4434 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2559..2658) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2675..2694) /label=T7 /note="T7 promoter, forward primer" promoter complement(2676..2694) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2699..2715) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2699..2715) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2817..2836) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2828..3637 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2895..2914) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3784..4372 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4273..4292 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggcac acgcaccaga gccagaccct gcagcatgcg acctgggcga 721 tgagagacca aagtgggata acaaggccca gtacctgctg agctgcaccg gattcgcagt 781 gggactgggc aatatctgga ggtttcctta cctgtgccag acatatggcg gcggcgcctt 841 cctgatcccc tatgtgatcg ccctggtgtt cgagggcatc cctatctttc acgtggagct 901 ggcaatcgga cagaggctga ggaagggatc cgtgggcgtg tggaccgcaa tctctcctta 961 cctgagcgga gtgggactgg gatgcgtgac cctgtccttc ctgatctctc tgtactataa 1021 cacaatcgtg gcctgggtgc tgtggtatct gctgaattcc tttcagcacc cactgccatg 1081 gagctcctgc ccacctgacc tgaaccgcac aggcttcgtg gaggagtgtc agggctctag 1141 cgccgtgtct tacttttggt atcggcagac cctgaacatc acagccgaca tcaatgattc 1201 cggctctatc cagtggtggc tgctgatctg cctggcagca agctgggccg tggtgtacat 1261 gtgcgtgatc cgcggcatcg agaccacagg caaagtgatc tacttcaccg ccctgtttcc 1321 atatctggtg ctgacaatct tcctgatcag gggactgacc ctgccaggag ccacaaaggg 1381 cctgatctac ctgtttaccc caaacatgca catcctgcag aatcccagag tgtggctgga 1441 tgccgccaca cagatcttct ttagcctgtc cctggccttc ggcggacaca tcgcctttgc 1501 cagctataac tccccccgca atgactgcca gaaggatgcc gtggtcatcg ccctggtgaa 1561 ccggatgacc agcctgtacg cctccatcgc cgtgttctct gtgctgggct ttaaggccac 1621 aaatgactat gagcactgtc tggatcggaa catcctgagc ctgatcaatg acttcgattt 1681 tcctgagcag tctatcagca gagacgatta cccagccgtg ctgatgcacc tgaatgcaac 1741 ctggccaaag agggtggcac agctgcctct gaaggcatgc ctgctggagg acttcctgga 1801 taagtccgcc tctggaccag gactggcctt cgtggtgttt accgagacag acctgcacat 1861 gccaggagca cccgtgtggg ccatgctgtt ctttggcatg ctgttcaccc tgggcctgag 1921 caccatgttt ggcacagtgg aggccgtgat cacaccactg ctggatgtgg gcgtgctgcc 1981 aagatgggtg ccaaaggagg ccctgaccgg actggtgtgc ctggtgtgct tcctgtctgc 2041 cacctgcttt acactgcaga gcggcaacta ctggctggag atcttcgaca actttgccgc 2101 ctcccctaat ctgctgatgc tggccttcct ggaggtggtg ggcgtggtgt acgtgtatgg 2161 catgaagagg ttttgtgacg atatcgcatg gatgaccggc cggagacctt ccccatactg 2221 gaggctgacc tggcgcgtgg tgtctcctct gctgctgaca atcttcgtgg cctatatcat 2281 cctgctgttt tggaagccac tgcgctacaa ggcctggaat cccaagtatg agctgttccc 2341 tagcagacag gagaagctgt atccaggatg ggcaagggca gcatgcgtgc tgctgtccct 2401 gctgcctgtg ctgtgggtgc cagtggccgc cctggcacag ctgctgacca ggcgccggag 2461 aacatggagg gacagagatg caaggccaga caccgatatg agacccgaca ccgatacaag 2521 gcctgacacc gatatgcgcc cagacacaga tatgcgggac ccagctttct tgtacaaagt 2581 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 2641 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt 2701 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca 2761 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 2821 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga 2881 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 2941 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 3001 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 3061 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 3121 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 3181 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 3241 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 3301 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 3361 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 3421 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 3481 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 3541 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 3601 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta 3661 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct 3721 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3901 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 3961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt //