LOCUS Exported 4083 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC7A7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4083) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4083) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4083 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2208..2307) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2324..2343) /label=T7 /note="T7 promoter, forward primer" promoter complement(2325..2343) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2348..2364) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2348..2364) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2466..2485) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2477..3286 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2544..2563) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3433..4021 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3922..3941 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggtgg actccaccga gtacgaggtg gcctctcagc cagaggtgga 721 gacaagccca ctgggcgatg gagcatcccc aggacctgag caggtgaagc tgaagaagga 781 gatcagcctg ctgaacggag tatgcctcat agtgggtaat atgatcggct ctggcatctt 841 tgtgagcccc aagggcgtgc tgatctactc tgccagcttc ggcctgagcc tggtcatctg 901 ggcagtgggc ggcctgttct ccgtgtttgg cgccctgtgc tatgcagagc tgggaaccac 961 aatcaagaag tctggcgcca gctacgccta tatcctggag gccttcggcg gctttctggc 1021 cttcatccgg ctgtggacat ctctgctgat catcgagcca accagccagg ccatcatcgc 1081 catcacattc gccaactaca tggtgcagcc cctgtttcct tcctgcttcg ccccctatgc 1141 agcatctaga ctgctggcag cagcatgcat ctgtctgctg acctttatca attgtgccta 1201 cgtgaagtgg ggcacactgg tgcaggacat cttcacctat gccaaggtgc tggccctgat 1261 cgccgtgatc gtggcaggaa tcgtgcggct gggacaggga gcatctacac actttgagaa 1321 cagcttcgag ggcagctcct ttgccgtggg cgacatcgcc ctggccctgt acagcgccct 1381 gttctcctat tctggctggg ataccctgaa ttacgtgaca gaggagatca agaacccaga 1441 gaggaatctg cccctgtcca tcggcatctc tatgcctatc gtgaccatca tctatatcct 1501 gacaaacgtg gcctactata ccgtgctgga catgagggat atcctggcat ccgacgcagt 1561 ggcagtgacc tttgccgatc agatctttgg catcttcaac tggatcatcc ctctgagcgt 1621 ggccctgtcc tgcttcggag gcctgaatgc atccatcgtg gcagcctcta ggctgttctt 1681 tgtgggaagc agggagggac acctgccaga cgccatctgc atgatccacg tggagaggtt 1741 tacaccagtg ccctccctgc tgttcaacgg catcatggcc ctgatctacc tgtgcgtgga 1801 ggatatcttt cagctgatca attactatag cttctcctac tggttctttg tgggcctgtc 1861 catcgtgggc cagctgtatc tgaggtggaa ggagcctgac aggcctaggc cactgaagct 1921 gagcgtgttc ttcccaatcg tgttttgcct gtgcaccatc ttcctggtgg ccgtgcccct 1981 gtacagcgat acaatcaact ccctgatcgg aatcgcaatc gccctgtctg gactgccttt 2041 ctattttctg atcatccggg tgcctgagca caagagacca ctgtacctgc ggagaatcgt 2101 gggctctgcc acccggtatc tgcaggtgct gtgcatgagc gtggcagcag agatggacct 2161 ggaggatgga ggcgagatgc ccaagcagag agatcctaag tccaatgacc cagctttctt 2221 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac 2281 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta 2341 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta 2401 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag 2461 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc 2521 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg 2581 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg 2641 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga 2701 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact 2761 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg 2821 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg 2881 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa 2941 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca 3001 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg 3061 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt 3121 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg 3181 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga 3241 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa 3301 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc 3361 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat 3421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 3481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 3541 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 3601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 3661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 3721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 3781 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 3841 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 3901 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 3961 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 4021 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 4081 gtt //