LOCUS Exported 11533 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression of myc-tagged HLA-A*02:01. ACCESSION . VERSION . KEYWORDS pCEP4-myc-HLA-A2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11533) AUTHORS McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG TITLE Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection. JOURNAL Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 10.1073/pnas.1915562116. Epub 2019 Dec 3. PUBMED 31796585 REFERENCE 2 (bases 1 to 11533) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11533 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 14..148 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(38..57) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 92..111 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(290..312) /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 898..2687 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(2993..4918) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(5353..5371) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5439..5543 /gene="bla" /label=AmpR promoter CDS 5544..6404 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5762..5781) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6575..7163 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7064..7083 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 7780..7925 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 7965..8990 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" primer_bind complement(9011..9030) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 9055..9103 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" enhancer 9756..10135 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 10136..10339 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 10290..10310 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 10336..10360 /label=LNCX /note="Human CMV promoter, forward primer" CDS 10442..10471 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" ORIGIN 1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac 61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt 121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca 181 ggttcagggg gaggtgggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg 241 gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 301 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 361 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc ggtcgactct 421 agagccccgc cgccggacga actaaacctg actacggcat ctctgcccct tcttcgctgg 481 tacgaggagc gcttttgttt tgtattcggg gcagtgcatg taatcccttc agttggttgg 541 tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg 601 ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt 661 ggctttcatc ctggagcaga ctttgcatgc tgtggactgc aacacaacat tgcctttatg 721 tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc 781 aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa 841 gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac 901 gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag 961 catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag 1021 gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt 1081 ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc 1141 agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata 1201 actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga 1261 cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat 1321 ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata 1381 tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca 1441 cacgaattta tggctatggg caacacataa tcctagtgca atatgatact ggggttatta 1501 agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac 1561 aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc 1621 gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt 1681 ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg 1741 gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca 1801 aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg 1861 cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc 1921 tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg 1981 gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta 2041 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta 2101 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta 2161 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta 2221 atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata tgctatccta 2281 atttatatct gggtagcata tactacccaa atatctggat agcatatgct atcctaatct 2341 atatctgggt agcatatgct atcctaatct atatctgggt agcataggct atcctaatct 2401 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt 2461 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct 2521 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc 2581 atatacagtc agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc 2641 gccacctccc aagggggcgt gaattttcgc tgcttgtcct tttcctgcat gctggttgct 2701 cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac 2761 caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac 2821 gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca 2881 gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc 2941 gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta catcactcct 3001 gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct 3061 ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa 3121 agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct 3181 taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac 3241 agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg 3301 ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta 3361 ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt 3421 cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt 3481 tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt 3541 tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat 3601 ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt 3661 cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg 3721 gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc 3781 tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc 3841 tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc 3901 ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc 3961 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg 4021 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg 4081 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc 4141 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg 4201 cccctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg 4261 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctgct cctgcccctc 4321 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc 4381 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc 4441 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc 4501 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc 4561 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg 4621 ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa 4681 tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg 4741 agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc 4801 catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg 4861 tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga 4921 ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag 4981 tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca 5041 gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt 5101 actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc 5161 caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac 5221 ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag 5281 ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt 5341 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 5401 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 5461 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 5521 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 5581 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 5641 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 5701 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 5761 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca 5821 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 5881 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 5941 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 6001 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 6061 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa 6121 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 6181 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 6241 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 6301 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 6361 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 6421 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 6481 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 6541 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 6601 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 6661 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 6721 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 6781 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 6841 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 6901 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 6961 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 7021 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 7081 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 7141 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 7201 tttgctgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg 7261 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt 7321 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca 7381 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac 7441 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc 7501 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct 7561 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga 7621 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc 7681 gcgtcggccg ccatgccctg cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg 7741 tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc 7801 gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc 7861 acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg 7921 cttaacagcg tcaacagcgt gccgcagatc ccgggcaatg agatatgaaa aagcctgaac 7981 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga 8041 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat 8101 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc 8161 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga 8221 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa 8281 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg 8341 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta 8401 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga 8461 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg 8521 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga 8581 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc 8641 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga 8701 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata 8761 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg 8821 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc 8881 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg 8941 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag gggagatggg 9001 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 9061 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg 9121 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 9181 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 9241 aacgtcgggg cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc 9301 ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtctgg 9361 tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca 9421 tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa 9481 aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct 9541 tatcatcgca gatccgggca acgttgttgc attgctgcag gcgcagaact ggtaggtatg 9601 gaagatctat acattgaatc aatattggca attagccata ttagtcattg gttatatagc 9661 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat 9721 ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta 9781 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 9841 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 9901 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 9961 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc 10021 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 10081 acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 10141 gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag 10201 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 10261 aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga 10321 ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagaagct gggtaccagc 10381 tgctagcgcc accatgaaga ccatcatcgc cctgagctac atcttctgcc tggtgttcgc 10441 cgagcagaaa ttgatcagcg aggaggacct gggcagccct ggaggcagtt caggaggcgg 10501 atctcactcc atgaggtatt tcttcacatc cgtgtcccgg cccggccgcg gggagccccg 10561 cttcatcgca gtgggctacg tggacgacac gcagttcgtg cggttcgaca gcgacgccgc 10621 gagccagagg atggagccgc gggcgccgtg gatagagcag gagggtccgg agtattggga 10681 cggggagaca cggaaagtga aggcccactc acagactcac cgagtggacc tggggaccct 10741 gcgcggctac tacaaccaga gcgaggccgg ttctcacacc gtccagagga tgtatggctg 10801 cgacgtgggg tcggactggc gcttcctccg cgggtaccac cagtacgcct acgacggcaa 10861 ggattacatc gccctgaaag aggacctgcg ctcttggacc gcggcggaca tggcagctca 10921 gaccaccaag cacaagtggg aggcggccca tgtggcggag cagttgagag cctacctgga 10981 gggcacgtgc gtggagtggc tccgcagata cctggagaac gggaaggaga cgctgcagcg 11041 tacggacgcc cccaaaacgc atatgactca ccacgctgtc tctgaccatg aagccaccct 11101 gaggtgctgg gccctgagct tctaccctgc ggagatcaca ctgacctggc agcgggatgg 11161 ggaggaccag acccaggaca cggagctcgt ggagaccagg cctgcagggg atggaacctt 11221 ccagaagtgg gcggctgtgg tggtgccttc tggacaggag cagagataca cctgccatgt 11281 gcagcatgag ggtttgccca agcccctcac cctgagatgg gagccgtctt cccagcccac 11341 catccccatc gtgggcatca ttgctggcct ggttctcttt ggagctgtga tcactggagc 11401 tgtggtcgct gctgtgatgt ggaggaggaa gagctcagat agaaaaggag ggagctactc 11461 tcaggctgca agcagtgaca gtgcccaggg ctctgatgtg tctctcacag cttgtaaagt 11521 gtgataactc gag //