LOCUS Exported 774 bp ds-DNA linear SYN 05-JUL-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pWZL Neo Myr Flag CDK2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 774) AUTHORS Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC TITLE Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene (Kinase Library Clones) JOURNAL Cell. 2007 Jun 15. 129(6):1065-1079. PUBMED 17574021 REFERENCE 2 (bases 1 to 774) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 5, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..774 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 35..44 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 41..82 /codon_start=1 /product="N-myristoylation signal from Src kinase (Pellman et al., 1985; Kaplan et al., 1988)" /label=myr /translation="MGSSKSKPKDPSQR" CDS 107..130 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" protein_bind 137..161 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa 61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga 121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atggagaact tccaaaaggt 181 ggaaaagatc ggagagggca cgtacggagt tgtgtacaaa gccagaaaca agttgacggg 241 agaggtggtg gcgcttaaga aaatccgcct ggacactgag actgagggtg tgcccagtac 301 tgccatccga gagatctctc tgcttaagga gcttaaccat cctaatattg tcaagctgct 361 ggatgtcatc cacacagaaa ataaactcta cctggttttt gaatttctgc accaagatct 421 caagaaattc atggatgcct ctgctctcac tggcattcct cttcccctca tcaagagcta 481 tctgttccag ctgctccagg gcctagcttt ctgccattct catcgggtcc tccaccgaga 541 ccttaaacct cagaatctgc ttattaacac agagggggcc atcaagctag cagactttgg 601 actagccaga gcttttggag tccctgttcg tacttacacc catgangtgg tgaccctgtg 661 gtaccgagct cctgaaatcc tcctgggctg cnaatattat tccacagctg tggacatctg 721 gagcctgggc tgcatctttg ctganatggt gactcgcngg gccctattcc ctgg //