LOCUS       Exported                 693 bp ds-DNA     linear   SYN 29-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWZL Neo Myr Flag CERK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      35..44
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgcaacgtg gccctgcttc
      181 aggtgatccg cgggaggggc ctccacgcca gcgccgggaa ggctgctggg gcctccacac
      241 ctgcctcatc acggcggcga ggctacgaca atccggctgg gagcatgacc ttggcgtctg
      301 ttctgggagc acggatgata agctctggaa gctggcagtg tgtaaagcac tggcaagttt
      361 gttactgtta aaatgtcaaa taccaatgct ttatatcgac gcgaagtgct taacacagcc
      421 gggcttgggg gcagtcagga ggaagctggc catccgtgga ggaggggccg gtcctggact
      481 cccgcaggac tcctctgagg cagggcctga agtctgtaca cgtggtccag atttgtcctt
      541 gtcttttctt cacactgagt tctctatatt tattgaacat cttgtccttt taagccagag
      601 tagtgtaaac tgcgtctcgg atgtctgtct tttgcctcga agccacgatg gatcgctggt
      661 ttcctctgca gcgcgagggc tccggcgacc aga
//
