LOCUS Exported 4716 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLCO4A1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4716) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4716) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4716 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2841..2940) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2957..2976) /label=T7 /note="T7 promoter, forward primer" promoter complement(2958..2976) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2981..2997) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2981..2997) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3099..3118) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 3110..3919 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(3177..3196) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 4066..4654 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4555..4574 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgccac tgcaccagct gggcgacaag cccctgacct ttcctagccc 721 aaactccgcc atggagaatg gcctggatca caccccacct tctaggagag caagcccagg 781 aacaccactg tctcctggaa gcctgcggtc cgccgcacac tccccactgg acacctctaa 841 gcagcctctg tgccagctgt gggcagagaa gcacggagca aggggcacac acgaggtgag 901 atacgtgtcc gccggacagt ctgtggcatg cggatggtgg gccttcgccc caccatgtct 961 gcaggtgctg aacaccccca agggcatcct gttcttcctg tgcgcagcag ccttcctgca 1021 gggaatgaca gtgaacggct ttatcaatac cgtgatcaca agcctggaga ggcgctacga 1081 cctgcactct tatcagagcg gcctgatcgc cagctcctat gatatcgccg cctgcctgtg 1141 cctgaccttc gtgagctact ttggaggatc cggacacaag cctaggtggc tgggatgggg 1201 cgtgctgctg atgggaaccg gatccctggt gttcgcactg ccacacttta cagccggcag 1261 gtatgaggtg gagctggacg caggcgtgcg cacctgccca gcaaatccag gagccgtgtg 1321 cgcagattcc acatctggcc tgagccgcta ccagctggtg ttcatgctgg gacagtttct 1381 gcacggagtg ggagcaacac cactgtacac cctgggcgtg acatatctgg acgagaacgt 1441 gaagtctagc tgctccccag tgtacatcgc catcttttat accgcagcaa tcctgggacc 1501 agcagcagga tatctgatcg gaggcgccct gctgaatatc tacaccgaga tgggccggag 1561 aacagagctg accacagagt ccccactgtg ggtgggagca tggtgggtgg gcttcctggg 1621 atctggagca gcagccttct ttacagcagt gccaatcctg ggctatccca gacagctgcc 1681 tggcagccag aggtacgccg tgatgagggc agcagagatg caccagctga aggattcctc 1741 taggggagag gcaagcaacc cagacttcgg caagaccatc cgcgatctgc ctctgtccat 1801 ctggctgctg ctgaagaatc ccaccttcat cctgctgtgc ctggcaggag caaccgaggc 1861 cacactgatc accggcatgt ccaccttctc tcctaagttt ctggagtccc agttcagcct 1921 gtccgcctct gaggcagcca ccctgtttgg ctatctggtg gtgccagcag gaggaggagg 1981 cacatttctg ggcggcttct ttgtgaacaa gctgaggctg cgcggctctg ccgtgatcaa 2041 gttctgcctg ttttgtaccg tggtgagcct gctgggcatc ctggtgttct ctctgcactg 2101 ccctagcgtg ccaatggcag gagtgaccgc aagctatgga ggatccctgc tgcctgaggg 2161 acacctgaac ctgacagccc catgcaatgc cgcctgctct tgtcagccag agcactattc 2221 ccccgtgtgc ggctctgacg gcctgatgta ctttagcctg tgccacgcag gatgtccagc 2281 agcaaccgag acaaacgtgg acggacagaa ggtgtaccgg gattgctcct gtatccctca 2341 gaatctgagc tccggattcg gacacgcaac cgccggcaag tgcacctcta catgtcagag 2401 aaagcccctg ctgctggtgt tcatctttgt ggtcatcttc ttcacattcc tgtctagcat 2461 ccccgccctg accgccacac tgagatgcgt gagagatcct cagcggagct ttgccctggg 2521 catccagtgg atcgtggtga gaatcctggg aggaatccca ggacctatcg ccttcggctg 2581 ggtcatcgac aaggcctgtc tgctgtggca ggatcagtgc ggccagcagg gctcctgtct 2641 ggtgtaccag aatagcgcca tgtccaggta tatcctgatc atgggcctgc tgtacaaggt 2701 gctgggcgtg ctgttctttg ccatcgcctg cttcctgtac aagcctctga gcgagtcctc 2761 tgatggcctg gagacctgtc tgccttccca gagctccgcc ccagactctg ccacagatag 2821 ccagctgcag tctagcgtgg acccagcttt cttgtacaaa gttggcatta taagaaagca 2881 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg 2941 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca 3001 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc 3061 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat 3121 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta 3181 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa 3241 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac 3301 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca 3361 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc 3421 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt 3481 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt 3541 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt 3601 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt 3661 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt 3721 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata 3781 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg 3841 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat 3901 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac 3961 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc 4021 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4081 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4141 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4201 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4261 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4321 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4381 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4441 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 4501 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 4561 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 4621 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 4681 gttcctggcc ttttgctggc cttttgctca catgtt //