LOCUS Exported 4401 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC2A12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4401) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4401) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4401 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2526..2625) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2642..2661) /label=T7 /note="T7 promoter, forward primer" promoter complement(2643..2661) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2666..2682) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2666..2682) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2784..2803) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2795..3604 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2862..2881) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3751..4339 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4240..4259 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggtgc ctgtggagaa taccgagggc ccatccctgc tgaaccagaa 721 gggcaccgcc gtggagacag agggaagcgg atccaggcac ccaccttggg ccagaggctg 781 cggcatgttc acctttctga gctccgtgac agcagccgtg tccggactgc tggtgggata 841 cgagctgggc atcatctctg gcgccctgct gcagatcaag accctgctgg ccctgagctg 901 tcacgagcag gagatggtgg tgtctagcct ggtcatcggc gccctgctgg catccctgac 961 cggaggcgtg ctgatcgaca gatatggccg gagaacagcc atcatcctgt cctcttgcct 1021 gctgggcctg ggctctctgg tgctgatcct gtctctgagc tacaccgtgc tgatcgtggg 1081 ccgcatcgcc atcggcgtgt ccatctctct gagctccatc gccacatgcg tgtacatcgc 1141 agagatcgca cctcagcaca ggaggggact gctggtgagc ctgaatgagc tgatgatcgt 1201 gatcggcatc ctgtccgcct acatctctaa ttatgccttc gccaacgtgt ttcacggctg 1261 gaagtacatg ttcggactgg tcatcccact gggcgtgctg caggcaatcg ccatgtattt 1321 cctgccaccc agccctcggt tcctggtcat gaagggacag gagggagcag catctaaggt 1381 gctgggccgg ctgagagccc tgagcgacac cacagaggag ctgaccgtga tcaagtctag 1441 cctgaaggat gagtaccagt atagcttctg ggacctgttt agatccaagg ataacatgag 1501 gacccgcatc atgatcggcc tgacactggt gttctttgtg cagatcaccg gccagccaaa 1561 tatcctgttc tacgcctcca cagtgctgaa gtctgtgggc tttcagagca acgaggcagc 1621 atctctggca agcaccggag tgggcgtggt gaaagtgatc tctaccatcc cagccacact 1681 gctggtggac cacgtgggca gcaagacatt tctgtgcatc ggctcctctg tgatggccgc 1741 ctccctggtg accatgggca tcgtgaatct gaacatccac atgaatttca cacacatctg 1801 taggtcccac aattctatca accagagcct ggacgagtcc gtgatctacg gccccggcaa 1861 cctgtccacc aacaataaca cactgcggga tcactttaag ggcatcagct cccactctag 1921 atctagcctg atgcctctga ggaatgacgt ggataagagg ggagagacca caagcgcctc 1981 cctgctgaac gcaggactga gccacaccga gtaccagatc gtgacagacc caggcgatgt 2041 gcccgccttc ctgaagtggc tgagcctggc ctccctgctg gtgtatgtgg ccgcctttag 2101 catcggcctg ggccctatgc catggctggt gctgtccgag atcttcccag gaggaatcag 2161 gggaagagca atggcactga cctcctctat gaattggggc atcaacctgc tgatctccct 2221 gacattcctg accgtgacag atctgatcgg cctgccttgg gtgtgcttta tctacaccat 2281 catgtctctg gccagcctgc tgttcgtggt catgtttatc ccagagacaa agggctgctc 2341 cctggagcag atctctatgg agctggccaa ggtgaactac gtgaagaaca acatctgttt 2401 catgagccac caccaggagg agctggtgcc caagcagcct cagaagcgga agccacagga 2461 gcagctgctg gagtgcaaca agctgtgcgg aaggggacag tctaggcagc tgagccccga 2521 gaccgaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg 2581 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat 2641 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 2701 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 2761 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 2821 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 2881 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 2941 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 3001 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 3061 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 3121 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 3181 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 3241 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 3301 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 3361 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 3421 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 3481 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 3541 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 3601 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 3661 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 3721 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 3781 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 3841 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 3901 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 3961 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 4021 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 4081 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 4141 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 4201 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 4261 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 4321 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 4381 ctggcctttt gctcacatgt t //