LOCUS Exported 4494 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC2A13 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4494) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4494) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4494 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2619..2718) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2735..2754) /label=T7 /note="T7 promoter, forward primer" promoter complement(2736..2754) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2759..2775) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2759..2775) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2877..2896) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2888..3697 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2955..2974) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3844..4432 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4333..4352 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgtcta gaaaggccag cgagaacgtg gagtacaccc tgaggagcct 721 gagctccctg atgggcgagc ggagaaggaa gcagcctgag ccagacgcag caagcgccgc 781 aggcgagtgt tccctgctgg cagcagcaga gtctagcacc agcctgcagt ccgccggagc 841 aggaggagga ggagtgggcg acctggagcg cgccgcccgc cggcagttcc agcaggatga 901 gacccctgcc ttcgtgtacg tggtggccgt gttttccgcc ctgggaggct tcctgtttgg 961 ctatgacaca ggcgtggtgt ctggcgccat gctgctgctg aagcggcagc tgagcctgga 1021 tgccctgtgg caggagctgc tggtgtcctc taccgtggga gcagcagccg tgtctgccct 1081 ggcaggcggc gccctgaatg gcgtgttcgg cagaagggca gcaatcctgc tggcatctgc 1141 cctgtttacc gcaggaagcg ccgtgctggc agcagcaaac aataaggaga cactgctggc 1201 aggcagactg gtggtgggac tgggaatcgg aatcgcatcc atgaccgtgc ccgtgtacat 1261 cgccgaggtg tctcccccta acctgagagg caggctggtg accatcaata cactgttcat 1321 cacaggcggc cagttctttg cctccgtggt ggacggcgcc ttttcttacc tgcagaagga 1381 tggctggagg tatatgctgg gactggcagc agtgcctgcc gtgatccagt tctttggctt 1441 cctgtttctg cctgagagcc caagatggct gatccagaag ggacagaccc agaaggcaag 1501 gaggatcctg agccagatga ggggcaacca gacaatcgac gaggagtacg attccatcaa 1561 gaacaatatc gaggaggagg agaaggaagt gggatccgcc ggaccagtga tctgcagaat 1621 gctgtcttat ccacccacca gaagggccct gatcgtgggc tgtggcctgc agatgttcca 1681 gcagctgtcc ggcatcaaca ccatcatgta ctatagcgcc acaatcctgc agatgtccgg 1741 cgtggaggac gataggctgg ccatctggct ggcctctgtg accgccttca caaatttcat 1801 ctttaccctg gtgggcgtgt ggctggtgga gaaagtgggc cggagaaagc tgacatttgg 1861 cagcctggca ggaaccacag tggccctgat catcctggcc ctgggattcg tgctgtccgc 1921 ccaggtgtct ccccgcatca cctttaagcc aatcgcacct agcggacaga acgcaacctg 1981 cacacggtac tcctattgca atgagtgtat gctggaccct gattgcggct tctgttacaa 2041 gatgaacaag tctaccgtga tcgacagctc ctgcgtgcca gtgaacaagg caagcacaaa 2101 tgaggcagca tggggccgct gtgagaacga gaccaagttt aagacagagg atatcttctg 2161 ggcctacaat ttttgcccca ccccttattc ctggacagcc ctgctgggcc tgatcctgta 2221 cctggtgttc tttgcaccag gaatgggacc aatgccatgg accgtgaact ctgagatcta 2281 tccactgtgg gcacggagca caggaaatgc atgttctagc ggcatcaact ggatcttcaa 2341 tgtgctggtg agcctgacct ttctgcacac agccgagtac ctgacctact atggcgcctt 2401 ctttctgtat gcaggattcg cagcagtggg actgctgttt atctacggct gcctgcccga 2461 gacaaagggc aagaagctgg aggagatcga gtccctgttc gacaatagac tgtgcacctg 2521 tggcacatcc gactctgatg agggccgcta catcgagtat atccgggtga agggcagcaa 2581 ctatcacctg tccgacaatg atgcctctga tgtggaggac ccagctttct tgtacaaagt 2641 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 2701 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt 2761 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca 2821 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 2881 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga 2941 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 3001 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 3061 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 3121 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 3181 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 3241 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 3301 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 3361 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 3421 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 3481 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 3541 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 3601 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 3661 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta 3721 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct 3781 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3841 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3901 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3961 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 4021 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4081 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4141 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4201 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4261 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4321 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4381 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4441 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt //