LOCUS Exported 4194 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SVOP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4194) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4194) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4194 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2319..2418) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2435..2454) /label=T7 /note="T7 promoter, forward primer" promoter complement(2436..2454) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2459..2475) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2459..2475) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2577..2596) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2588..3397 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2655..2674) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3544..4132 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4033..4052 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggagg aggacctgtt ccagctgcgc cagctgcccg tggtgaagtt 721 tcggagaacc ggagagagcg cccggtccga ggacgataca gcaagcggag agcacgaggt 781 gcagatcgag ggagtgcacg tgggactgga ggcagtggag ctggacgatg gagcagccgt 841 gcctaaggag ttcgccaacc caaccgacga taccttcatg gtggaggacg cagtggaggc 901 aatcggattc ggcaagtttc agtggaagct gtctgtgctg accggactgg catggatggc 961 agatgcaatg gagatgatga tcctgagcat cctggccccc cagctgcact gtgagtggag 1021 actgcctagc tggcaggtgg ccctgctgac ctccgtggtg ttcgtgggca tgatgagctc 1081 ctctacactg tggggcaata tcagcgacca gtacggcagg aagaccggcc tgaagatctc 1141 cgtgctgtgg acactgtact atggcatcct gagcgccttt gccccagtgt actcctggat 1201 cctggtgctg aggggactgg tgggattcgg aatcggagga gtgccacagt ccgtgaccct 1261 gtatgccgag tttctgccca tgaaggcccg ggccaagtgt atcctgctga tcgaggtgtt 1321 ttgggccatc ggcacagtgt tcgaggtggt gctggccgtg tttgtgatgc cctctctggg 1381 ctggagatgg ctgctgatcc tgagcgccgt gcctctgctg ctgttcgccg tgctgtgctt 1441 ttggctgcca gagtctgcca gatatgatgt gctgagcggc aaccaggaga aggccatcgc 1501 caccctgaag aggatcgcca cagagaatgg cgcccccatg cctctgggca agctgatcat 1561 cagccggcag gaggacagag gcaagatgag ggatctgttc acccctcact ttcgctggac 1621 cacactgctg ctgtggttca tctggttttc caacgccttc tcttactatg gcctggtgct 1681 gctgaccaca gagctgtttc aggcaggcga cgtgtgcgga atcagctccc ggaagaaggc 1741 agtggaggca aagtgctccc tggcctgtga gtacctgtct gaggaggact atatggatct 1801 gctgtggacc acactgtccg agttcccagg cgtgctggtg accctgtgga tcatcgatag 1861 gctgggccgc aagaagacaa tggccctgtg cttcgtgatc ttctcttttt gtagcctgct 1921 gctgtttatc tgcgtgggcc gcaatgtgct gaccctgctg ctgttcatcg cccgggcctt 1981 tatctccggc ggcttccagg ccgcctacgt gtatacacca gaggtgtacc caaccgcaac 2041 aagagccctg ggactgggca cctgttctgg aatggcaagg gtgggcgccc tgatcacacc 2101 cttcatcgcc caggtcatgc tggagtctag cgtgtacctg accctggccg tgtattccgg 2161 atgctgtctg ctggccgccc tggcatcttg ctttctgcct atcgagacaa agggaagggg 2221 actgcaggag tcctctcaca gggagtgggg acaggagatg gtgggaagag gaatgcacgg 2281 agcaggagtg accaggagca actccggctc tcaggaggac ccagctttct tgtacaaagt 2341 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 2401 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt 2461 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca 2521 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 2581 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga 2641 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 2701 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 2761 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 2821 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 2881 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 2941 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 3001 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 3061 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 3121 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 3181 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 3241 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 3301 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 3361 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta 3421 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct 3481 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 3541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 3601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 3661 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 3721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 3781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 3841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 3901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 3961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt //