LOCUS Exported 13729 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression plasmid for c-myc-tagged PDGFRB. ACCESSION . VERSION . KEYWORDS pCEP4-myc-PDGFRbeta SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13729) AUTHORS Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, Procko E TITLE Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. JOURNAL PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun. PUBMED 32559251 REFERENCE 2 (bases 1 to 13729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13729 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 61..90 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1555..1701 /codon_start=1 /product="transmembrane domain from platelet derived growth factor receptor beta" /label=PDGFR-beta TM domain /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ KKPR" polyA_signal 3362..3496 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3386..3405) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3440..3459 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(3638..3660) /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 4246..6035 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(6341..8266) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(8701..8719) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8787..8891 /gene="bla" /label=AmpR promoter CDS 8892..9752 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9110..9129) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9923..10511 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10412..10431 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 11128..11273 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 11313..12338 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" primer_bind complement(12359..12378) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 12403..12451 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" enhancer 13104..13483 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 13484..13687 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 13638..13658 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 13684..13708 /label=LNCX /note="Human CMV promoter, forward primer" ORIGIN 1 gctagcgcca ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc 61 gagcagaaat tgatcagcga ggaggacctg ggcagccctg gaggcgcctc tggcctggtc 121 gtcacacccc cggggccaga gcttgtcctc aatgtctcca gcaccttcgt tctgacctgc 181 tcgggttcag ctccggtggt gtgggaacgg atgtcccagg agcccccaca ggaaatggcc 241 aaggcccagg atggcacctt ctccagcgtg ctcacactga ccaacctcac tgggctagac 301 acgggagaat acttttgcac ccacaatgac tcccgtggac tggagaccga tgagcggaaa 361 cggctctaca tctttgtgcc agatcccacc gtgggcttcc tccctaatga tgccgaggaa 421 ctattcatct ttctcacgga aataactgag atcaccattc catgccgagt aacagaccca 481 cagctggtgg tgacactgca cgagaagaaa ggggacgttg cactgcctgt cccctatgat 541 caccaacgtg gcttttctgg tatctttgag gacagaagct acatctgcaa aaccaccatt 601 ggggacaggg aggtggattc tgatgcctac tatgtctaca gactccaggt gtcatccatc 661 aacgtctctg tgaacgcagt gcagactgtg gtccgccagg gtgagaacat caccctcatg 721 tgcattgtga tcgggaatga ggtggtcaac ttcgagtgga catacccccg caaagaaagt 781 gggcggctgg tggagccggt gactgacttc ctcttggata tgccttacca catccgctcc 841 atcctgcaca tccccagtgc cgagttagaa gactcgggga cctacacctg caatgtgacg 901 gagagtgtga atgaccatca ggatgaaaag gccatcaaca tcaccgtggt tgagagcggc 961 tacgtgcggc tcctgggaga ggtgggcaca ctacaatttg ctgagctgca tcggagccgg 1021 acactgcagg tagtgttcga ggcctaccca ccgcccactg tcctgtggtt caaagacaac 1081 cgcaccctgg gcgactccag cgctggcgaa atcgccctgt ccacgcgcaa cgtgtcggag 1141 acccggtatg tgtcagagct gacactggtt cgcgtgaagg tggcagaggc tggccactac 1201 accatgcggg ccttccatga ggatgctgag gtccagctct ccttccagct acagatcaat 1261 gtccctgtcc gagtgctgga gctaagtgag agccaccctg acagtgggga acagacagtc 1321 cgctgtcgtg gccggggcat gccccagccg aacatcatct ggtctgcctg cagagacctc 1381 aaaaggtgtc cacgtgagct gccgcccacg ctgctgggga acagttccga agaggagagc 1441 cagctggaga ctaacgtgac gtactgggag gaggagcagg agtttgaggt ggtgagcaca 1501 ctgcgtctgc agcacgtgga tcggccactg tcggtgcgct gcacgctgcg caacgctgtg 1561 ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa ggtggtggtg 1621 atctcagcca tcctggccct ggtggtgctc accatcatct cccttatcat cctcatcatg 1681 ctttggcaga agaagccacg ttacgagatc cgatggaagg tgattgagtc tgtgagctct 1741 gacggccatg agtacatcta cgtggacccc atgcagctgc cctatgactc cacgtgggag 1801 ctgccgcggg accagcttgt gctgggacgc accctcggct ctggggcctt tgggcaggtg 1861 gtggaggcca cggctcatgg cctgagccat tctcaggcca cgatgaaagt ggccgtcaag 1921 atgcttaaat ccacagcccg cagcagtgag aagcaagccc ttatgtcgga gctgaagatc 1981 atgagtcacc ttgggcccca cctgaacgtg gtcaacctgt tgggggcctg caccaaagga 2041 ggacccatct atatcatcac tgagtactgc cgctacggag acctggtgga ctacctgcac 2101 cgcaacaaac acaccttcct gcagcaccac tccgacaagc gccgcccgcc cagcgcggag 2161 ctctacagca atgctctgcc cgttgggctc cccctgccca gccatgtgtc cttgaccggg 2221 gagagcgacg gtggctacat ggacatgagc aaggacgagt cggtggacta tgtgcccatg 2281 ctggacatga aaggagacgt caaatatgca gacatcgagt cctccaacta catggcccct 2341 tacgataact acgttccctc tgcccctgag aggacctgcc gagcaacttt gatcaacgag 2401 tctccagtgc taagctacat ggacctcgtg ggcttcagct accaggtggc caatggcatg 2461 gagtttctgg cctccaagaa ctgcgtccac agagacctgg cggctaggaa cgtgctcatc 2521 tgtgaaggca agctggtcaa gatctgtgac tttggcctgg ctcgggacat catgcgggac 2581 tcgaattaca tctccaaagg cagcaccttt ttgcctttaa agtggatggc tccggagagc 2641 atcttcaaca gcctctacac caccctgagc gacgtgtggt ccttcgggat cctgctctgg 2701 gagatcttca ccttgggtgg caccccttac ccagagctgc ccatgaacga gcagttctac 2761 aatgccatca aacggggtta ccgcatggcc cagcctgccc atgcctccga cgagatctat 2821 gagatcatgc agaagtgctg ggaagagaag tttgagattc ggcccccctt ctcccagctg 2881 gtgctgcttc tggagagact gttgggcgaa ggttacaaaa agaagtacca gcaggtggat 2941 gaggagtttc tgaggagtga ccacccagcc atccttcggt cccaggcccg cttgcctggg 3001 ttccatggcc tccgatctcc cctggacacc agctccgtcc tctatactgc cgtgcagccc 3061 aatgagggtg acaacgacta tatcatcccc ctgcctgacc ccaaacccga ggttgctgac 3121 gagggcccac tggagggttc ccccagccta gccagctcca ccctgaatga agtcaacacc 3181 tcctcaacca tctcctgtga cagccccctg gagccccagg acgaaccaga gccagagccc 3241 cagcttgagc tccaggtgga gccggagcca gagctggaac agttgccgga ttcggggtgc 3301 cctgcgcctc gggcggaagc agaggatagc ttcctgtaat gactcgaggc cggcaaggcc 3361 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 3421 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 3481 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 3541 ggtggggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat 3601 ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 3661 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 3721 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccgg tcgactctag agccccgccg 3781 ccggacgaac taaacctgac tacggcatct ctgccccttc ttcgctggta cgaggagcgc 3841 ttttgttttg tattcggggc agtgcatgta atcccttcag ttggttggta caacttgcca 3901 actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact 3961 gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct 4021 ggagcagact ttgcatgctg tggactgcaa cacaacattg cctttatgtg taactcttgg 4081 ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg 4141 ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa 4201 gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg 4261 cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc 4321 aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat 4381 ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag 4441 tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc 4501 tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg 4561 tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat 4621 aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca 4681 aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata 4741 gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg 4801 gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca 4861 ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag 4921 agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg 4981 gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt 5041 gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata 5101 agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc 5161 acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata 5221 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc 5281 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc 5341 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg 5401 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg 5461 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg 5521 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg 5581 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg 5641 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag 5701 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag 5761 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag 5821 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag 5881 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag 5941 catatgatac ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa 6001 gggggcgtga attttcgctg cttgtccttt tcctgcatgc tggttgctcc cattcttagg 6061 tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc 6121 gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg 6181 gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga tggccagaaa 6241 tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg gggaatacac 6301 ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc ccttcctcac 6361 cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc gcggcagccc 6421 cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag ctgcacacag 6481 tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta atcgcatcct 6541 tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag acaatggact 6601 cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg agacgactca 6661 atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg 6721 aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg tcggtagtcc 6781 tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg 6841 ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg cgcctgcctc 6901 catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct gcggggccct 6961 gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct tggtggtatt 7021 caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga gggggcctgc 7081 gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc ttttctccac 7141 gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt tcacgtcctc 7201 taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct cgaccccggc 7261 ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct gctcctgccc 7321 ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc 7381 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc 7441 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc 7501 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc 7561 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc 7621 ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct cctgctcctg 7681 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc 7741 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc 7801 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg 7861 cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc 7921 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc 7981 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac 8041 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg 8101 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac 8161 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct 8221 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa 8281 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg 8341 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg 8401 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg 8461 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat 8521 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc 8581 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc 8641 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg 8701 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 8761 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 8821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 8881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 8941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 9001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 9061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 9121 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc 9181 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 9241 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 9301 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 9361 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 9421 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 9481 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 9541 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 9601 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 9661 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 9721 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 9781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 9841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 9901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 9961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 10021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 10081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 10141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 10201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 10261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 10321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 10381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 10441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 10501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctgcgccg 10561 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc 10621 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc 10681 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac 10741 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg 10801 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg 10861 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac 10921 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg 10981 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc 11041 atgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa 11101 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg 11161 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt 11221 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc 11281 aacagcgtgc cgcagatccc gggcaatgag atatgaaaaa gcctgaactc accgcgacgt 11341 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg 11401 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg 11461 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg 11521 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt 11581 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg 11641 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga 11701 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt 11761 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg 11821 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg 11881 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc 11941 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg 12001 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg 12061 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg 12121 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc 12181 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg 12241 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa 12301 accgacgccc cagcactcgt ccgagggcaa aggaataggg gagatggggg aggctaactg 12361 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 12421 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 12481 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 12541 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 12601 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg 12661 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtctggtc cacgactgga 12721 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg 12781 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg 12841 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga 12901 tccgggcaac gttgttgcat tgctgcaggc gcagaactgg taggtatgga agatctatac 12961 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat 13021 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc 13081 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat 13141 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 13201 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 13261 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 13321 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt 13381 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc 13441 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 13501 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 13561 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa 13621 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 13681 cagagctcgt ttagtgaacc gtcagatctc tagaagctgg gtaccagct //