LOCUS Exported 4225 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pUC19/human IL-8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4225) AUTHORS Matsushima K, Morishita K, Yoshimura T, Lavu S, Kobayashi Y, Lew W, Appella E, Kung HF, Leonard EJ, Oppenheim JJ TITLE Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. JOURNAL J Exp Med. 1988 Jun 1. 167(6):1883-93. PUBMED 3260265 REFERENCE 2 (bases 1 to 4225) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4225 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 490..509 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 743..760 /label=L4440 /note="L4440 vector, forward primer" protein_bind 877..898 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 913..943 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 951..967 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 956..978 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 975..991 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 975..991 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" misc_feature 1004..1060 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(2600..2617) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2600..2616) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2609..2631) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(2825..2844) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 2944..2966 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3004..3022) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3090..3194 /gene="bla" /label=AmpR promoter CDS 3195..4055 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLTSRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3413..3432) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca 1021 ggtcgactct agaggatccc cgggtaccga gctcgaattc actagagcac acaagcttct 1081 aggacaagag ccaggaagaa accaccggaa ggaaccatct cactgtgtgt aaacatgact 1141 tccaagctgg ccgtggctct cttggcagcc ttcctgattt ctgcagctct gtgtgaaggt 1201 gcagttttgc caaggagtgc taaagaactt agatgtcagt gcataaagac atactccaaa 1261 cctttccacc ccaaatttat caaagaactg agagtgattg agagtggacc acactgcgcc 1321 aacacagaaa ttattgtaaa gctttctgat ggaagagagc tctgtctgga ccccaaggaa 1381 aactgggtgc agagggttgt ggagaagttt ttgaagaggg ctgagaattc ataaaaaaat 1441 tcattctctg tggtatccaa gaatcagtga agatgccagt gaaacttcaa gcaaatctac 1501 ttcaacactt catgtattgt gtgggtctgt tgtagggttg ccagatgcaa tacaagattc 1561 ctggttaaat ttgaatttca gtaaacaatg aatagttttt cattgtacca tgaaatatcc 1621 agaacatact tatatgtaaa gtattattta tttgaatcta caaaaaacaa caaataattt 1681 ttaaatataa ggattttcct agatattgca cgggagaata tacaaatagc aaaattgagg 1741 ccaagggcca agagaatatc cgaactttaa tttcaggaat tgaatgggtt tgctagaatg 1801 tgatatttga agcatcacat aaaaatgatg ggacaataaa ttttgccata aagtcaaatt 1861 tagctggaaa tcctggattt ttttctgtta aatctggcaa ccctagtctg ctagccagga 1921 tccacaagtc cttgttccac tgtgccttgg tttctccttt atttctaagt ggaaaaagta 1981 ttagccacca tcttacctca cagtgatgtt gtgaggacat gtggaagcac tttaagtttt 2041 ttcatcataa cataaattat tttcaagtgt aacttattaa cctatttatt atttatgtat 2101 ttatttaagc atcaaatatt tgtgcaagaa tttggaaaaa tagaagatga atcattgatt 2161 gaatagttat aaagatgtta tagtaaattt attttatttt agatattaaa tgatgtttta 2221 ttagataaat ttcaatcagg gtttttagat taaacaaaca aacaattggg tacccagtta 2281 aattttcatt tcagatatac aacaaataat tttttagtat aagtacatta ttgtttatct 2341 gaaattttaa ttgaactaac aatcctagtt tgatactccc agtcttgtca ttgccagctg 2401 tgttggtagt gctgtgttga attacggaat aatgagttag aactattaaa acagccaaaa 2461 ctccacagtc aatattagta atttcttgct ggttgaaact tgtttattat gtacaaatag 2521 attcttataa tattatttaa atgactgcat ttttaaatac aaggctttat atttttaact 2581 ttaaaaaaaa ccggaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 2641 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 2701 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc 2761 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc 2821 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 2881 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 2941 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 3001 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 3061 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 3121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 3181 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 3241 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 3301 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 3361 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 3421 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 3481 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 3541 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 3601 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 3661 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 3721 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 3781 tacttactct aacttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 3841 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 3901 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 3961 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 4021 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 4081 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 4141 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 4201 ccgtagaaaa gatcaaagga tcttc //