LOCUS       Exported                1266 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  Lentiviral expression vector for an inducible p53(R280K)-V5.
ACCESSION   .
VERSION     .
KEYWORDS    pSLIK_hygro_p53(R280K)_V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..1179
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGKD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     CDS             1213..1254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggaggagc cgcagtcaga tcctagcgtc gagccccctc tgagtcagga aacattttca
       61 gacctatgga aactacttcc tgaaaacaac gttctgtccc ccttgccgtc ccaagcaatg
      121 gatgatttga tgctgtcccc ggacgatatt gaacaatggt tcactgaaga cccaggtcca
      181 gatgaagctc ccagaatgcc agaggctgct ccccccgtgg cccctgcacc agcagctcct
      241 acaccggcgg cccctgcacc agccccctcc tggcccctgt catcttctgt cccttcccag
      301 aaaacctacc agggcagcta cggtttccgt ctgggcttct tgcattctgg gacagccaag
      361 tctgtgactt gcacgtactc ccctgccctc aacaagatgt tttgccaact ggccaagacc
      421 tgccctgtgc agctgtgggt tgattccaca cccccgcccg gcacccgcgt ccgcgccatg
      481 gccatctaca agcagtcaca gcacatgacg gaggttgtga ggcgctgccc ccaccatgag
      541 cgctgctcag atagcgatgg tctggcccct cctcagcatc ttatccgagt ggaaggaaat
      601 ttgcgtgtgg agtatttgga tgacagaaac acttttcgac atagtgtggt ggtgccctat
      661 gagccgcctg aggttggctc tgactgtacc accatccact acaactacat gtgtaacagt
      721 tcctgcatgg gcggcatgaa ccggaggccc atcctcacca tcatcacact ggaagactcc
      781 agtggtaatc tactgggacg gaacagcttt gaggtgcgtg tttgtgcctg tcctgggaaa
      841 gaccggcgca cagaggaaga gaatctccgc aagaaagggg agcctcacca cgagctgccc
      901 ccagggagca ctaagcgagc actgcccaac aacaccagct cctctcccca gccaaagaag
      961 aaaccactgg atggagaata tttcaccctt cagatccgtg ggcgtgagcg cttcgagatg
     1021 ttccgagagc tgaatgaggc cttggaactc aaggatgccc aggctgggaa ggagccaggg
     1081 gggagcaggg ctcactccag ccacctgaag tccaaaaagg gtcagtctac ctcccgccat
     1141 aaaaaactca tgttcaagac agaagggcct gactcagaca agggctcgag tctagagggc
     1201 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     1261 ggttag
//
