LOCUS Exported 11320 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc. ACCESSION . VERSION . KEYWORDS pHAGE2-TetOminiCMV-OSKM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11320) AUTHORS Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR TITLE Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. JOURNAL Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 10.1016/j.stem.2019.10.002. PUBMED 31708402 REFERENCE 2 (bases 1 to 11320) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11320 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2251..2521 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2554..2592 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2589..2613 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 2679..2688 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3756..3812 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 4791..4844 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6315..6374 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=E2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" misc_feature 7762..8350 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7815..7835) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8233..8244) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8498..8678 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8725..8743) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8811..8915 /gene="bla" /label=AmpR promoter CDS 8916..9776 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9134..9153) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9947..10535 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10436..10455 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10689..10706 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10798..10817 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10946..10967 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10982..11012 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11020..11036 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11025..11047 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11044..11060 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11044..11060 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11110..11129 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca 2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat 2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg 2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc 2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa 2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg 2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg 2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct 3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc 3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc 3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg 3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca 3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca 3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa 3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc 3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg 3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt 3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc 3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac 3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg 3721 gctctcccat gcattcaaac gctagcggca gcggcgccac gaacttctct ctgttaaagc 3781 aagcaggaga tgttgaagaa aaccccgggc ctgcttgcat gtataacatg atggagacgg 3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca 3901 cggcggcggc gaccggcggc aaccagaaga acagcccgga ccgcgtcaag aggcccatga 3961 acgccttcat ggtatggtcc cgggggcagc ggcgtaagat ggcccaggag aaccccaaga 4021 tgcacaactc ggagatcagc aagcgcctgg gcgcggagtg gaaacttttg tccgagaccg 4081 agaagcggcc gttcatcgac gaggccaagc ggctgcgcgc tctgcacatg aaggagcacc 4141 cggattataa ataccggccg cggcggaaaa ccaagacgct catgaagaag gataagtaca 4201 cgcttcccgg aggcttgctg gcccccggcg ggaacagcat ggcgagcggg gttggggtgg 4261 gcgccggcct gggtgcgggc gtgaaccagc gcatggacag ctacgcgcac atgaacggct 4321 ggagcaacgg cagctacagc atgatgcagg agcagctggg ctacccgcag cacccgggcc 4381 tcaacgctca cggcgcggca cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc 4441 agtacaactc catgaccagc tcgcagacct acatgaacgg ctcgcccacc tacagcatgt 4501 cctactcgca gcagggcacc cccggtatgg cgctgggctc catgggctct gtggtcaagt 4561 ccgaggccag ctccagcccc cccgtggtta cctcttcctc ccactccagg gcgccctgcc 4621 aggccgggga cctccgggac atgatcagca tgtacctccc cggcgccgag gtgccggagc 4681 ccgctgcgcc cagtagactg cacatggccc agcactacca gagcggcccg gtgcccggca 4741 cggccattaa cggcacactg cccctgtcgc acatggcatg cggctccggc gagggcaggg 4801 gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccactcgag atgaggcagc 4861 cacctggcga gtctgacatg gctgtcagcg acgctctgct cccgtccttc tccacgttcg 4921 cgtccggccc ggcgggaagg gagaagacac tgcgtccagc aggtgccccg actaaccgtt 4981 ggcgtgagga actctctcac atgaagcgac ttcccccact tcccggccgc ccctacgacc 5041 tggcggcgac ggtggccaca gacctggaga gtggcggagc tggtgcagct tgcagcagta 5101 acaacccggc cctcctagcc cggagggaga ccgaggagtt caacgacctc ctggacctag 5161 actttatcct ttccaactcg ctaacccacc aggaatcggt ggccgccacc gtgaccacct 5221 cggcgtcagc ttcatcctcg tcttccccag cgagcagcgg ccctgccagc gcgccctcca 5281 cctgcagctt cagctatccg atccgggccg ggggtgaccc gggcgtggct gccagcaaca 5341 caggtggagg gctcctctac agccgagaat ctgcgccacc tcccacggcc cccttcaacc 5401 tggcggacat caatgacgtg agcccctcgg gcggcttcgt ggctgagctc ctgcggccgg 5461 agttggaccc agtatacatt ccgccacagc agcctcagcc gccaggtggc gggctgatgg 5521 gcaagtttgt gctgaaggcg tctctgacca cccctggcag cgagtacagc agcccttcgg 5581 tcatcagtgt tagcaaagga agcccagacg gcagccaccc cgtggtagtg gcgccctaca 5641 gcggtggccc gccgcgcatg tgccccaaga ttaagcaaga ggcggtcccg tcctgcacgg 5701 tcagccggtc cctagaggcc catttgagcg ctggacccca gctcagcaac ggccaccggc 5761 ccaacacaca cgacttcccc ctggggcggc agctccccac caggactacc cctacactga 5821 gtcccgagga actgctgaac agcagggact gtcaccctgg cctgcctctt cccccaggat 5881 tccatcccca tccggggccc aactaccctc ctttcctgcc agaccagatg cagtcacaag 5941 tcccctctct ccattatcaa gagctcatgc caccgggttc ctgcctgcca gaggagccca 6001 agccaaagag gggaagaagg tcgtggcccc ggaaaagaac agccacccac acttgtgact 6061 atgcaggctg tggcaaaacc tataccaaga gttctcatct caaggcacac ctgcgaactc 6121 acacaggcga gaaaccttac cactgtgact gggacggctg tgggtggaaa ttcgcccgct 6181 ccgatgaact gaccaggcac taccgcaaac acacagggca ccggcccttt cagtgccaga 6241 agtgcgacag ggccttttcc aggtcggacc accttgcctt acacatgaag aggcactttc 6301 tcgagggctc gggccagtgt actaattatg ctctcttgaa attggctgga gatgttgaga 6361 gcaacccagg tcccggcgcg ccactggatt tcctttgggc gttggaaacc ccgcagacag 6421 ccacgacgat gcccctcaac gtgaacttca ccaacaggaa ctatgacctc gactacgact 6481 ccgtacagcc ctatttcatc tgcgacgagg aagagaattt ctatcaccag caacagcaga 6541 gcgagctgca gccgcccgcg cccagtgagg atatctggaa gaaattcgag ctgcttccca 6601 ccccgcccct gtccccgagc cgccgctccg ggctctgctc tccatcctat gttgcggtcg 6661 ctacgtcctt ctccccaagg gaagacgatg acggcggcgg tggcaacttc tccaccgccg 6721 atcagctgga gatgatgacc gagttacttg gaggagacat ggtgaaccag agcttcatct 6781 gcgatcctga cgacgagacc ttcatcaaga acatcatcat ccaggactgt atgtggagcg 6841 gtttctcagc cgctgccaag ctggtctcgg agaagctggc ctcctaccag gctgcgcgca 6901 aagacagcac cagcctgagc cccgcccgcg ggcacagcgt ctgctccacc tccagcctgt 6961 acctgcagga cctcaccgcc gccgcgtccg agtgcattga cccctcagtg gtctttccct 7021 acccgctcaa cgacagcagc tcgcccaaat cctgtacctc gtccgattcc acggccttct 7081 ctccttcctc ggactcgctg ctgtcctccg agtcctcccc acgggccagc cctgagcccc 7141 tagtgctgca tgaggagaca ccgcccacca ccagcagcga ctctgaagaa gagcaagaag 7201 atgaggaaga aattgatgtg gtgtctgtgg agaagaggca aacccctgcc aagaggtcgg 7261 agtcgggctc atctccatcc cgaggccaca gcaaacctcc gcacagccca ctggtcctca 7321 agaggtgcca cgtctccact caccagcaca actacgccgc acccccctcc acaaggaagg 7381 actatccagc tgccaagagg gccaagttgg acagtggcag ggtcctgaag cagatcagca 7441 acaaccgcaa gtgctccagc cccaggtcct cagacacgga ggaaaacgac aagaggcgga 7501 cacacaacgt cttggaacgt cagaggagga acgagctgaa gcgcagcttt tttgccctgc 7561 gtgaccagat ccctgaattg gaaaacaacg aaaaggcccc caaggtagtg atcctcaaaa 7621 aagccaccgc ctacatcctg tccattcaag cagacgagca caagctcacc tctgaaaagg 7681 acttattgag gaaacgacga gaacagttga aacacaaact cgaacagctt cgaaactctg 7741 gtgcataaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact 7801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 7861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 7921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 7981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 8041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 8101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 8161 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 8221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 8281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 8341 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 8401 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca 8461 cctgcaggac aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat 8521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 8581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 8641 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc 8701 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 8761 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 8821 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 8881 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 8941 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 9001 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 9061 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 9121 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 9181 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 9241 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 9301 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 9361 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 9421 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 9481 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 9541 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 9601 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 9661 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 9721 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 9781 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 9841 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 9901 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 9961 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 10021 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 10081 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 10141 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 10201 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 10261 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 10321 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 10381 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 10441 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 10501 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 10561 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 10621 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 10681 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 10741 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat 10801 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 10861 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga 10921 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 10981 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 11041 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc 11101 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 11161 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 11221 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 11281 gaaattgtat ttgttaaata tgtactacaa acttagtagt //