LOCUS       Exported               10186 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Dox-inducible polycistronic reprogramming lentiviral vector 
            expressing mouse Sox2, Klf4, cMyc.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE2-TetOminiCMV-SKM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
  TITLE     Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental 
            Potential of iPSCs.
  JOURNAL   Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002.
  PUBMED    31708402
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2251..2521
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2554..2592
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3657..3710
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5181..5240
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    6628..7216
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6681..6701)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7099..7110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7364..7544
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7591..7609)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7677..7781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7782..8642
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8000..8019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8813..9401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9302..9321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9555..9572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9664..9683
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    9812..9833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9848..9878
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9886..9902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9891..9913
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9910..9926
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9910..9926
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9976..9995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatgtat aacatgatgg
     2701 agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc ggaggaggca
     2761 acgccacggc ggcggcgacc ggcggcaacc agaagaacag cccggaccgc gtcaagaggc
     2821 ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc caggagaacc
     2881 ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa cttttgtccg
     2941 agaccgagaa gcggccgttc atcgacgagg ccaagcggct gcgcgctctg cacatgaagg
     3001 agcacccgga ttataaatac cggccgcggc ggaaaaccaa gacgctcatg aagaaggata
     3061 agtacacgct tcccggaggc ttgctggccc ccggcgggaa cagcatggcg agcggggttg
     3121 gggtgggcgc cggcctgggt gcgggcgtga accagcgcat ggacagctac gcgcacatga
     3181 acggctggag caacggcagc tacagcatga tgcaggagca gctgggctac ccgcagcacc
     3241 cgggcctcaa cgctcacggc gcggcacaga tgcaaccgat gcaccgctac gacgtcagcg
     3301 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3361 gcatgtccta ctcgcagcag ggcacccccg gtatggcgct gggctccatg ggctctgtgg
     3421 tcaagtccga ggccagctcc agcccccccg tggttacctc ttcctcccac tccagggcgc
     3481 cctgccaggc cggggacctc cgggacatga tcagcatgta cctccccggc gccgaggtgc
     3541 cggagcccgc tgcgcccagt agactgcaca tggcccagca ctaccagagc ggcccggtgc
     3601 ccggcacggc cattaacggc acactgcccc tgtcgcacat ggcatgcggc tccggcgagg
     3661 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcgagatga
     3721 ggcagccacc tggcgagtct gacatggctg tcagcgacgc tctgctcccg tccttctcca
     3781 cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt gccccgacta
     3841 accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc ggccgcccct
     3901 acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt gcagcttgca
     3961 gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac gacctcctgg
     4021 acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc gccaccgtga
     4081 ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct gccagcgcgc
     4141 cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc gtggctgcca
     4201 gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc acggccccct
     4261 tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct gagctcctgc
     4321 ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca ggtggcgggc
     4381 tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag tacagcagcc
     4441 cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg gtagtggcgc
     4501 cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg gtcccgtcct
     4561 gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc agcaacggcc
     4621 accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg actaccccta
     4681 cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg cctcttcccc
     4741 caggattcca tccccatccg gggcccaact accctccttt cctgccagac cagatgcagt
     4801 cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc ctgccagagg
     4861 agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc acccacactt
     4921 gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag gcacacctgc
     4981 gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg tggaaattcg
     5041 cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg ccctttcagt
     5101 gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac atgaagaggc
     5161 actttctcga gggctcgggc cagtgtacta attatgctct cttgaaattg gctggagatg
     5221 ttgagagcaa cccaggtccc ggcgcgccac tggatttcct ttgggcgttg gaaaccccgc
     5281 agacagccac gacgatgccc ctcaacgtga acttcaccaa caggaactat gacctcgact
     5341 acgactccgt acagccctat ttcatctgcg acgaggaaga gaatttctat caccagcaac
     5401 agcagagcga gctgcagccg cccgcgccca gtgaggatat ctggaagaaa ttcgagctgc
     5461 ttcccacccc gcccctgtcc ccgagccgcc gctccgggct ctgctctcca tcctatgttg
     5521 cggtcgctac gtccttctcc ccaagggaag acgatgacgg cggcggtggc aacttctcca
     5581 ccgccgatca gctggagatg atgaccgagt tacttggagg agacatggtg aaccagagct
     5641 tcatctgcga tcctgacgac gagaccttca tcaagaacat catcatccag gactgtatgt
     5701 ggagcggttt ctcagccgct gccaagctgg tctcggagaa gctggcctcc taccaggctg
     5761 cgcgcaaaga cagcaccagc ctgagccccg cccgcgggca cagcgtctgc tccacctcca
     5821 gcctgtacct gcaggacctc accgccgccg cgtccgagtg cattgacccc tcagtggtct
     5881 ttccctaccc gctcaacgac agcagctcgc ccaaatcctg tacctcgtcc gattccacgg
     5941 ccttctctcc ttcctcggac tcgctgctgt cctccgagtc ctccccacgg gccagccctg
     6001 agcccctagt gctgcatgag gagacaccgc ccaccaccag cagcgactct gaagaagagc
     6061 aagaagatga ggaagaaatt gatgtggtgt ctgtggagaa gaggcaaacc cctgccaaga
     6121 ggtcggagtc gggctcatct ccatcccgag gccacagcaa acctccgcac agcccactgg
     6181 tcctcaagag gtgccacgtc tccactcacc agcacaacta cgccgcaccc ccctccacaa
     6241 ggaaggacta tccagctgcc aagagggcca agttggacag tggcagggtc ctgaagcaga
     6301 tcagcaacaa ccgcaagtgc tccagcccca ggtcctcaga cacggaggaa aacgacaaga
     6361 ggcggacaca caacgtcttg gaacgtcaga ggaggaacga gctgaagcgc agcttttttg
     6421 ccctgcgtga ccagatccct gaattggaaa acaacgaaaa ggcccccaag gtagtgatcc
     6481 tcaaaaaagc caccgcctac atcctgtcca ttcaagcaga cgagcacaag ctcacctctg
     6541 aaaaggactt attgaggaaa cgacgagaac agttgaaaca caaactcgaa cagcttcgaa
     6601 actctggtgc ataaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     6661 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6721 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6781 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6841 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6901 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6961 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7021 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7081 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7141 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7201 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7261 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7321 agatcacctg caggacaggc gcgccctgct ttttgcttgt actgggtctc tctggttaga
     7381 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7441 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7501 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcacccggg cgattaagga
     7561 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7621 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     7681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7741 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7801 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7861 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7921 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7981 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     8041 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8101 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8161 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8221 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8281 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8341 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8401 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8461 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8521 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8581 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8641 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     9661 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9721 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     9781 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9841 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9901 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
     9961 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10021 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10081 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10141 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
