LOCUS Exported 4068 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC38A2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4068) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4068) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4068 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2193..2292) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2309..2328) /label=T7 /note="T7 promoter, forward primer" promoter complement(2310..2328) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2333..2349) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2333..2349) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2451..2470) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2462..3271 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2529..2548) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3418..4006 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3907..3926 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgaaga aggccgagat gggccggttc tctatcagcc ctgacgagga 721 tagctcctct tacagctcca actccgactt taattactct tatcccacca agcaggccgc 781 cctgaagtcc cactatgccg acgtggatcc tgagaaccag aatttcctgc tggagagcaa 841 cctgggcaag aagaagtacg agacagagtt tcacccaggc accacaagct tcggcatgtc 901 cgtgtttaac ctgtctaatg caatcgtggg aagcggaatc ctgggactgt cctatgccat 961 ggccaacacc ggcatcgccc tgttcatcat cctgctgaca ttcgtgagca tcttttccct 1021 gtactctgtg cacctgctgc tgaagaccgc caatgaggga ggaagcctgc tgtacgagca 1081 gctgggctat aaggccttcg gcctggtggg caagctggca gcatccggct ctatcacaat 1141 gcagaacatc ggcgccatgt ctagctacct gtttatcgtg aagtatgagc tgccactggt 1201 catccaggcc ctgaccaata tcgaggataa gacaggcctg tggtacctga acggcaatta 1261 tctggtgctg ctggtgtctc tggtggtcat cctgcccctg agcctgttca gaaatctggg 1321 ctacctgggc tataccagcg gcctgtccct gctgtgcatg gtgttctttc tgatcgtggt 1381 catctgcaag aagtttcagg tgccatgtcc tgtggaggcc gccctgatca tcaacgagac 1441 aatcaatacc acactgaccc agcctacagc cctggtgcca gccctgtccc acaacgtgac 1501 cgagaatgac tcttgcagac ctcactactt catctttaac tctcagacag tgtatgccgt 1561 gccaatcctg atcttcagct tcgtgtgcca cccagccgtg ctgcccatct acgaggagct 1621 gaaggatagg tcccggagaa ggatgatgaa cgtgagcaag atcagcttct ttgccatgtt 1681 cctgatgtat ctgctggccg ccctgttcgg ctacctgacc ttttatgagc acgtggagag 1741 cgagctgctg cacacctact cctctatcct gggcacagac atcctgctgc tgatcgtgcg 1801 cctggccgtg ctgatggcag tgaccctgac agtgcccgtg gtcatcttcc ctatccgcag 1861 ctccgtgacc cacctgctgt gcgcctctaa ggactttagc tggtggcggc acagcctgat 1921 caccgtgtcc atcctggcct tcacaaacct gctggtcatc tttgtgccca caatccggga 1981 tatcttcggc tttatcggcg cctccgccgc ctctatgctg atcttcatcc tgccatccgc 2041 cttttacatc aagctggtga agaaggagcc catgaagtct gtgcagaaga tcggcgccct 2101 gttctttctg ctgagcggcg tgctggtcat gaccggctcc atggccctga tcgtgctgga 2161 ttgggtgcac aatgcccctg gcggcggcca cgacccagct ttcttgtaca aagttggcat 2221 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa 2281 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc 2341 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa 2401 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 2461 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga 2521 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 2581 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 2641 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 2701 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 2761 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 2821 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 2881 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 2941 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 3001 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 3061 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 3121 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 3181 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 3241 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg 3301 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt 3361 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 3421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 3481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 3601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 3661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 3721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 3781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 3841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 3901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 3961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 4021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt //