LOCUS       Exported                 779 bp ds-DNA     linear   SYN 11-JUL-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWZL Neo Myr Flag NEK11
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      35..44
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgctgaaat tccaagaggc
      181 agctaagtgt gtgagtggat caacagccat ttccacttat ccaaagacct tgattgcaag
      241 aagatacgtg cttcaacaaa aacttggcag tggaagtttt ggaactgtct atctggtttc
      301 agacaagaaa gccaaacgag gagaggaatt aaaggtactt aaggaaatat ctgttggaga
      361 actaaatcca aatgaaactg tacaggccaa tttggaagcc caactcctct ccaagctgga
      421 ccacccagcc attgtcaagt tccatgcaag ttttgtggag caagataatt tctgcattat
      481 cacggagtac tgtgagggcc gagatctgga cgataaaatt caggaatata aacaagctgg
      541 aaaaatcttt ccagaaaatc aaataataga atggtttatc cagctgctgc tgggagttga
      601 ctacatgcat gagaggagga tacttcatcg agacttaaag tcaaagaatg tatttctgaa
      661 aaataatctc cttaaattgg agattttgga gtttctcgac ttctaatggg atcctgtgac
      721 ctggccacaa ctttaactgg aactccccat tatatgagtc ctgaggctct gaaacacca
//
