LOCUS Exported 10614 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Klf4, Sox2, cMyc. ACCESSION . VERSION . KEYWORDS pHAGE2-TetOminiCMV-KSM SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10614) AUTHORS Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR TITLE Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. JOURNAL Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 10.1016/j.stem.2019.10.002. PUBMED 31708402 REFERENCE 2 (bases 1 to 10614) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10614 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2251..2521 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2554..2592 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2589..2613 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 2679..2688 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 4113..4680 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4130..4682 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4274..4291) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4501..4520 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5663..5722 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=E2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" misc_feature 7056..7644 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7109..7129) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7527..7538) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7792..7972 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8019..8037) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8105..8209 /gene="bla" /label=AmpR promoter CDS 8210..9070 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8428..8447) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9241..9829 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9730..9749 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9983..10000 /label=L4440 /note="L4440 vector, forward primer" primer_bind 10092..10111 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 10240..10261 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10276..10306 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10314..10330 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10319..10341 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10338..10354 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10338..10354 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 10404..10423 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca 2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt 2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac 2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag 2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat 2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct gtcagcgacg 2701 ctctgctccc gtccttctcc acgttcgcgt ccggcccggc gggaagggag aagacactgc 2761 gtccagcagg tgccccgact aaccgttggc gtgaggaact ctctcacatg aagcgacttc 2821 ccccacttcc cggccgcccc tacgacctgg cggcgacggt ggccacagac ctggagagtg 2881 gcggagctgg tgcagcttgc agcagtaaca acccggccct cctagcccgg agggagaccg 2941 aggagttcaa cgacctcctg gacctagact ttatcctttc caactcgcta acccaccagg 3001 aatcggtggc cgccaccgtg accacctcgg cgtcagcttc atcctcgtct tccccagcga 3061 gcagcggccc tgccagcgcg ccctccacct gcagcttcag ctatccgatc cgggccgggg 3121 gtgacccggg cgtggctgcc agcaacacag gtggagggct cctctacagc cgagaatctg 3181 cgccacctcc cacggccccc ttcaacctgg cggacatcaa tgacgtgagc ccctcgggcg 3241 gcttcgtggc tgagctcctg cggccggagt tggacccagt atacattccg ccacagcagc 3301 ctcagccgcc aggtggcggg ctgatgggca agtttgtgct gaaggcgtct ctgaccaccc 3361 ctggcagcga gtacagcagc ccttcggtca tcagtgttag caaaggaagc ccagacggca 3421 gccaccccgt ggtagtggcg ccctacagcg gtggcccgcc gcgcatgtgc cccaagatta 3481 agcaagaggc ggtcccgtcc tgcacggtca gccggtccct agaggcccat ttgagcgctg 3541 gaccccagct cagcaacggc caccggccca acacacacga cttccccctg gggcggcagc 3601 tccccaccag gactacccct acactgagtc ccgaggaact gctgaacagc agggactgtc 3661 accctggcct gcctcttccc ccaggattcc atccccatcc ggggcccaac taccctcctt 3721 tcctgccaga ccagatgcag tcacaagtcc cctctctcca ttatcaagag ctcatgccac 3781 cgggttcctg cctgccagag gagcccaagc caaagagggg aagaaggtcg tggccccgga 3841 aaagaacagc cacccacact tgtgactatg caggctgtgg caaaacctat accaagagtt 3901 ctcatctcaa ggcacacctg cgaactcaca caggcgagaa accttaccac tgtgactggg 3961 acggctgtgg gtggaaattc gcccgctccg atgaactgac caggcactac cgcaaacaca 4021 cagggcaccg gccctttcag tgccagaagt gtgacagggc cttttccagg tcggaccacc 4081 ttgccttaca catgaagagg cacttttaaa gatccctccc ccccccctaa cgttactggc 4141 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 4201 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 4261 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 4321 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 4381 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 4441 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 4501 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 4561 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 4621 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 4681 tatggccaca catatgatgt ataacatgat ggagacggag ctgaagccgc cgggcccgca 4741 gcaagcttcg gggggcggcg gcggaggagg caacgccacg gcggcggcga ccggcggcaa 4801 ccagaagaac agcccggacc gcgtcaagag gcccatgaac gccttcatgg tatggtcccg 4861 ggggcagcgg cgtaagatgg cccaggagaa ccccaagatg cacaactcgg agatcagcaa 4921 gcgcctgggc gcggagtgga aacttttgtc cgagaccgag aagcggccgt tcatcgacga 4981 ggccaagcgg ctgcgcgctc tgcacatgaa ggagcacccg gattataaat accggccgcg 5041 gcggaaaacc aagacgctca tgaagaagga taagtacacg cttcccggag gcttgctggc 5101 ccccggcggg aacagcatgg cgagcggggt tggggtgggc gccggcctgg gtgcgggcgt 5161 gaaccagcgc atggacagct acgcgcacat gaacggctgg agcaacggca gctacagcat 5221 gatgcaggag cagctgggct acccgcagca cccgggcctc aacgctcacg gcgcggcaca 5281 gatgcaaccg atgcaccgct acgacgtcag cgccctgcag tacaactcca tgaccagctc 5341 gcagacctac atgaacggct cgcccaccta cagcatgtcc tactcgcagc agggcacccc 5401 cggtatggcg ctgggctcca tgggctctgt ggtcaagtcc gaggccagct ccagcccccc 5461 cgtggttacc tcttcctccc actccagggc gccctgccag gccggggacc tccgggacat 5521 gatcagcatg tacctccccg gcgccgaggt gccggagccc gctgcgccca gtagactgca 5581 catggcccag cactaccaga gcggcccggt gcccggcacg gccattaacg gcacactgcc 5641 cctgtcgcac atgggtagtg ggcaatgtac taactacgct ttgttgaaac tcgctggcga 5701 tgttgaaagt aaccccggtc ctatgcccct caacgtgaac ttcaccaaca ggaactatga 5761 cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga atttctatca 5821 ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt 5881 cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct gctctccatc 5941 ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg gcggtggcaa 6001 cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag acatggtgaa 6061 ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca tcatccagga 6121 ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc tggcctccta 6181 ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc 6241 cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca ttgacccctc 6301 agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta cctcgtccga 6361 ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct ccccacgggc 6421 cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca gcgactctga 6481 agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga ggcaaacccc 6541 tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac ctccgcacag 6601 cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg ccgcaccccc 6661 ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg gcagggtcct 6721 gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca cggaggaaaa 6781 cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc tgaagcgcag 6841 cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg cccccaaggt 6901 agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg agcacaagct 6961 cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca aactcgaaca 7021 gcttcgaaac tctggtgcat aaatcgatag atcctaatca acctctggat tacaaaattt 7081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 7141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 7201 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 7261 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 7321 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 7381 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 7441 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 7501 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 7561 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 7621 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct 7681 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 7741 cgaagacaag atcacctgca ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc 7801 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 7861 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 7921 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cacccgggcg 7981 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt 8041 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 8101 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 8161 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 8221 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 8281 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 8341 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 8401 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc 8461 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 8521 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 8581 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 8641 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 8701 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 8761 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 8821 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 8881 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 8941 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 9001 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 9061 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 9121 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 9181 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 9241 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 9301 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 9361 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 9421 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 9481 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 9541 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 9601 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 9661 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 9721 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 9781 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 9841 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 9901 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 9961 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 10021 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga 10081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 10141 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag 10201 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 10261 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 10321 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc 10381 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 10441 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc 10501 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt 10561 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //