LOCUS       Exported                7667 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Cre-dependant (flex) rabies glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    CAG-Flex-RG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     enhancer        231..534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        536..813
                     /label=chicken beta-actin promoter
     intron          815..1832
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1756..1778
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1840..1859
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1912..1945)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    complement(2008..2041)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      3619..3628
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    3635..3668
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    3739..3772
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3796..4384
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3849..3869)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4267..4278)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4408..4884
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4607..4626)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4930..5070
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4930..5059
                     /label=AAV2 ITR
     rep_origin      5145..5600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5232..5251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5442..5463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5617..5636)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5736..5758
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5796..5814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5882..5986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5987..6847
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6205..6224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7018..7606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7507..7526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaattc ccggggaatc tagttattaa
      181 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      241 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      301 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      361 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      421 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      481 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
      541 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
      601 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc
      661 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      721 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      781 cggccctata aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc
      841 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      901 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      961 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     1021 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     1081 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     1141 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1201 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1261 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     1321 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1381 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1441 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1501 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1561 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     1621 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1681 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     1741 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     1801 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     1861 tgtctcatca ttttggcaaa gaattgattt gataccgcgg gcccggccgg gataacttcg
     1921 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
     1981 cgaattgcca agcatcacca tcgacacata acttcgtata atgtatgcta tacgaagtta
     2041 tgtcgactta cagtctggtc tcacccccac tcttgtgtga ttcccatgaa gatatgatct
     2101 tcccgctttg gggagtgact gacacctccc tccctgtccc tctgagattg tgttgcgtag
     2161 gttctgatcg attgactctt ctacaacatg tcatcaggaa aattatcaac atcaaggcag
     2221 tcagggcccc tgcactcagt aatacatact tcccccagtt cgggagaccc aagtcaactc
     2281 ctgagacctg attgtgcaca tcgggaaggt gaacttcaac aaaatcctca gcctcgtcac
     2341 cgtccttgaa aacggtagac gggtctgcca gggggtgcac aagggggata accgaggatt
     2401 ccaacaactc catatgttgc tggaggaggg atgattgcat ctctgggatt aagacattgc
     2461 cgtcaggtcc taatattata ccattgaaaa acaccccgtt cacatgagga tgacacctcc
     2521 ccccaactct taaacaccct tttgaaggga ggatctcatt ccaagttctg actgacttgt
     2581 agtgagcatc ggcttccatc aaggtcttgt tgaatatggt atatgctttt ccaaacccag
     2641 ggacaagttt tcttaaatga ctgagacgtc tgaaactcac tgacttggtt gtcatgatgg
     2701 actctagtgc atccagacac tcctctctct tcctgaccaa ctcctctaca acaaggtgct
     2761 caatttcgtc tgagcgaaag tcgtgcaggt tcaccaactt atcgggaggg caccatttgg
     2821 tttcatttga tgtttgcatc gagacccatg ttccatccat aagtctaagt cctagaactc
     2881 cacataactt gagtttgcat gctcctttta aagacttata taggcctctt tcatctacaa
     2941 agccgcaagt ctcactccct ttggatgctc tcttccctct actattggta aaaatgtcac
     3001 aagacatccc tagtctcgga ttctcgggca tccaaatggt gtaatcgtgg ttagtggagc
     3061 agtaggtaga agacaccgct actcctgagc acttcccgct agggaagacc ctcgagtgaa
     3121 gggatctgtc atatgggtcc aaatctgcca cacttggaga tatgataacg agagactcct
     3181 tggtggtttt tacagttcga agccagcggt agtcagggta cggattgtgt agagactctt
     3241 catatctggg gtcaccggcc atcttccagt tgtacgcggc tctacatgca tctggtgttg
     3301 ggcggaaatg ctttcttttg aacgtggttg tgacataacc aacgaagtta gtgtaggttt
     3361 cagcctccgt cacaacgcct gtgcaagtga acccgttcac ttttatggct aagatgtatc
     3421 caactttaag ttccatgtag gagaaccctg acaggttggt gcatccttcg tcctccacta
     3481 ccaaattgtt tgggcagctg aggtgatgta tgtcaatcgg actccaggga ccaagcttgt
     3541 ctggtatcgt gtaaataggg aatttcccaa aacacaatgg aaaaaccaga aggggtacaa
     3601 acaggagagc ctgaggaacc atggtggcgc gcctataact tcgtatagga tactttatac
     3661 gaagttatca ttgggattct tcctattttg atccaagcat caccatcgac cctctagtcc
     3721 agatctcacc atcgacccat aacttcgtat agcatacatt atacgaagtt ataagcttat
     3781 atgcggccga tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3841 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3901 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3961 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4021 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4081 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4141 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4201 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4261 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4321 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4381 ccgcatcgat accgtagatc gatctacggg tggcatccct gtgacccctc cccagtgcct
     4441 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     4501 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     4561 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     4621 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     4681 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     4741 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     4801 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     4861 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgaactag
     4921 tgcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4981 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     5041 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     5101 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     5161 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5221 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5281 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5341 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5401 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5461 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     5521 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5581 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     5641 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5701 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5761 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5821 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5881 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5941 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6001 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6061 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6121 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6181 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6241 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6301 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6361 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6421 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6481 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6541 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6601 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6661 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6721 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6781 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6841 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6901 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6961 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7021 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7081 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7141 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7381 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7621 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
