LOCUS Exported 4173 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC2A10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4173) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4173) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4173 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2298..2397) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2414..2433) /label=T7 /note="T7 promoter, forward primer" promoter complement(2415..2433) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2438..2454) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2438..2454) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2556..2575) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2567..3376 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2634..2653) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3523..4111 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4012..4031 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgggac actccccacc tgtgctgcca ctgtgcgcat ccgtgtctct 721 gctgggagga ctgaccttcg gctacgagct ggccgtgatc tctggcgccc tgctgccact 781 gcagctggac tttggcctga gctgcctgga gcaggagttc ctggtgggat ccctgctgct 841 gggcgccctg ctggcctctc tggtgggcgg ctttctgatc gattgttatg gcaggaagca 901 ggccatcctg ggatccaacc tggtgctgct ggcaggatct ctgaccctgg gactggcagg 961 aagcctggca tggctggtgc tgggccgcgc cgtggtgggc ttcgccatca gcctgagctc 1021 catggcctgc tgtatctacg tgtccgagct ggtgggacct aggcagagag gcgtgctggt 1081 gtccctgtat gaggccggca tcaccgtggg catcctgctg tcttacgccc tgaattatgc 1141 actggcagga accccatggg gatggaggca catgtttgga tgggcaacag caccagccgt 1201 gctgcagagc ctgtccctgc tgttcctgcc tgcaggaaca gacgagaccg caacacacaa 1261 ggatctgatc cctctgcagg gaggagaggc accaaagctg ggacctggca ggccacgcta 1321 ctcctttctg gacctgttcc gggccagaga taacatgagg ggaaggacca cagtgggact 1381 gggactggtg ctgtttcagc agctgaccgg ccagcccaac gtgctgtgct atgcctccac 1441 aatcttttct agcgtgggat tccacggagg atcctctgcc gtgctggcat ctgtgggact 1501 gggagcagtg aaggtggcag ccaccctgac agcaatggga ctggtggaca gagcaggccg 1561 gagagccctg ctgctggcag gatgcgccct gatggccctg tctgtgagcg gaatcggact 1621 ggtgagcttc gccgtgccca tggatagcgg accttcctgt ctggcagtgc caaacgcaac 1681 cggacagaca ggcctgcctg gcgactccgg cctgctgcag gatagctccc tgccacccat 1741 cccaaggacc aatgaggacc agagggagcc aatcctgagc accgccaaga agacaaagcc 1801 acaccccagg agcggcgatc cctccgcccc tccacgcctg gccctgtcta gcgccctgcc 1861 aggaccacct ctgccagcaa ggggacacgc actgctgaga tggaccgccc tgctgtgcct 1921 gatggtgttc gtgagcgcct tcagcttcgg cttcggccct gtgacatggc tggtgctgtc 1981 tgagatctac ccagtggaga tccggggcag agcctttgcc ttctgtaaca gctttaattg 2041 ggccgccaac ctgtttatct ccctgtcttt cctggacctg atcggcacca tcggcctgag 2101 ctggacattc ctgctgtacg gactgaccgc cgtgctggga ctgggcttta tctatctgtt 2161 cgtgcccgag acaaagggcc agagcctggc cgagatcgat cagcagttcc agaagaggcg 2221 ctttaccctg tccttcggcc accggcagaa ttctacaggc atcccttata gcagaatcga 2281 gatctccgcc gcctctgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg 2341 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca 2401 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct 2461 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 2521 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 2581 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 2641 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 2701 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 2761 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 2821 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 2881 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 2941 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 3001 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 3061 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 3121 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 3181 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 3241 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 3301 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 3361 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 3421 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt 3481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 3541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 3601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 3661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 3721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 3781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 3841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 3901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 3961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4141 cctggccttt tgctggcctt ttgctcacat gtt //