LOCUS Exported 3120 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC31A1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3120) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3120) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3120 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1245..1344) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1361..1380) /label=T7 /note="T7 promoter, forward primer" promoter complement(1362..1380) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1385..1401) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1385..1401) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1503..1522) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1514..2323 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1581..1600) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2470..3058 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2959..2978 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggacc acagccacca catgggcatg tcctacatgg attctaacag 721 caccatgcag cccagccacc accaccctac cacatccgcc tctcacagcc acggaggagg 781 cgacagctcc atgatgatga tgccaatgac cttctatttt ggcttcaaga acgtggagct 841 gctgttttcc ggcctggtca tcaacacagc aggagagatg gcaggagcct ttgtggccgt 901 gttcctgctg gccatgttct acgagggcct gaagatcgcc cgggagtctc tgctgagaaa 961 gtcccaggtg tctatccggt ataacagcat gcccgtgcct ggcccaaatg gcaccatcct 1021 gatggagacc cacaagacag tgggccagca gatgctgtcc tttccccacc tgctgcagac 1081 agtgctgcac atcatccagg tggtcatctc ttactttctg atgctgatct tcatgaccta 1141 caatggctat ctgtgcatcg cagtggcagc aggagcagga acaggctatt ttctgttctc 1201 ttggaagaag gccgtggtgg tggatatcac agagcactgt cacgacccag ctttcttgta 1261 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat 1321 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta 1381 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat 1441 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 1501 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa 1561 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc 1621 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa 1681 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt 1741 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac 1801 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga 1861 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa 1921 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa 1981 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt 2041 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga 2101 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg 2161 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga 2221 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat 2281 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg 2341 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa 2401 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa 2461 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 2521 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 2581 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 2641 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 2701 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 2761 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 2821 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2881 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2941 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3001 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3061 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt //