LOCUS       Exported                 768 bp ds-DNA     linear   SYN 31-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWZL Neo Myr Flag PIK3R3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      35..44
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgtacaata cggtgtggag
      181 tatggaccgc gatgacgcag actggaggga ggtgatgatg ccctattcga cagaactgat
      241 attttatatt gaaatggatc ctccagctct tccaccaaag ccacctaagc caatgacttc
      301 agcagttcca aatggaatga aggacagttc tgtttctctt caggatgcag aatggtactg
      361 gggggatatt tcaagggagg aggtaaatga caaattgcgg gatatgccag atgggacctt
      421 cttggtccga gatgcctcaa caaaaatgca gggagattat actttgactt tgcggaaggg
      481 aggcaataat aagttaataa agatctatca ccgggatggt aaatatggct tttctgatcc
      541 tctgacattt aattccgtgg tggagctcat taaccactat caccatgaat ctcttgctca
      601 gtacaatccc aaacttgatg tgaagctgat gtacccagtg tccagatacc aacaggatca
      661 gttggtaaaa gaagataata ttgatgcagt aggtaaaaaa ctgcaagaat accactctca
      721 gtatcagaga agagtaaaga gtatgatagc tgtatgaaga atatacta
//
