LOCUS Exported 4307 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBiFC-bFOSDelataZipVC155 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4307) AUTHORS Shyu YJ, Liu H, Deng X, Hu CD TITLE Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. JOURNAL Biotechniques. 2006 Jan . 40(1):61-6. PUBMED 16454041 REFERENCE 2 (bases 1 to 4307) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4307 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 179..201 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 193..210 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 194..210 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 237..540 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 541..744 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 695..715 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 741..765 /label=LNCX /note="Human CMV promoter, forward primer" intron 882..978 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" CDS 1042..1068 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 1390..1641 /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VC155 /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" primer_bind 1578..1599 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1656..1790 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1680..1699) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1734..1753 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1901..1917) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1901..1917) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1914..1936) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1925..1941 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1949..1979) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1994..2015 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2132..2149) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2303..2891) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2383..2402) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3062..3922) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3685..3704 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3923..4027) /gene="bla" /label=AmpR promoter primer_bind 4095..4113 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(4151..4173) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4273..4292 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 61 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 121 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 181 cagtcacgac gttgtaaaac gacggccagt gagttcgagc ttgcatgcct gcaggtcgtt 241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat 781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagag 841 gatccggtac tagaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt 901 cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga 961 tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat 1021 tgtacccgcg ggcccaccat gtacccatac gatgttccag attacgctct tatggccatg 1081 gaggcccgaa ttcggggtcg tgcgcagtcc atcggtcgcc gcggtaaagt tgaacaactg 1141 tccccggaag aggaagagaa acgtcgcatc cgccgtgaac gtaacaaaat ggcggcagcg 1201 aaatgccgta accgccgtcg tgaactgacc gacaccctgc aggcggaaac cgaccagctg 1261 gaagacgaaa aatccgcgga gttcatcctg gcggcacacc gtccggcgtg caaaatcccg 1321 aacgacctgg gtggtacccg tccggcgtgc aaaatcccga acgacctgaa acagaaagtc 1381 atgaaccacg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc 1441 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 1501 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc 1561 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 1621 ggcatggacg agctgtacaa gtaagcggcc gcggggatcc agacatgata agatacattg 1681 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 1741 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 1801 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc 1861 tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt 1921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 1981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 2041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 2101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 2161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 2221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 2281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 2341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 2401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 2461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 2521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 2581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 2641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 2701 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 2761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 2881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 2941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 3001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 3061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 3121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 3181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 3241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 3301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 3361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 3421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 3481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 3541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 3601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 3661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 3721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 3781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 3841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 3901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 3961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 4021 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 4081 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 4141 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 4201 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 4261 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccat //