LOCUS       Exported                 735 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWZL Neo Myr Flag RPS6KA6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      35..44
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgctaccat tcgctcctca
      181 ggacgagccc tgggaccgag aaatggaagt gttcagcggc ggcggcgcga gcagcggcga
      241 ggtaaatggt cttaaaatgg ttgatgagcc aatggaagag ggagaagcag attcttgtca
      301 tgatgaagga gttgttaaag aaatccctat tactcatcat gttaaggaag gctatgagaa
      361 agcagatcct gcacagtttg agttgctcaa ggttcttggt caggggtcat ttggaaaggt
      421 ttttcttgtt agaaagaaga ccggtcctga tgctgggcag ctctatgcaa tgaaggtgtt
      481 aaaaaaagcc tctttaaaag ttcgagacag agttcggaca aagatggaga gggatatact
      541 ggtggaagta aatcatccat ttattgtcaa attgcactat gcctttcaga ctgaagggaa
      601 actgtactta atactggatt ttctcanggg aggagatgtt ttcacaagat tatccaaaga
      661 ggttctgttt acagaggaag atgtgaaatt ctacctcgca gaactggccc ttgctttgga
      721 tcatctgcac caatt
//
