LOCUS       Exported                 668 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWZL Neo Myr Flag TK1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      35..44
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgagctgca ttaacctgcc
      181 cactgtgctg cccggctccc ccagcaagac ccgggggcag atccaggtga ttctcgggcc
      241 gatgttctca ggaaaaagca cagagttgat gagacgcgtc cgtcgcttcc agattgctca
      301 gtacaagtgc ctggtgatca agtatgccaa agacactcgc tacagcagca gcttctgcac
      361 acatgaccgg aacaccatgg aggcgctgcc cgcctgcctg ctccgagacg tggcccagga
      421 ggccctgggc gtggctgtca taggcatcga cgaggggcag tttttccctg acatcgtgga
      481 gttctgcgag gccatggcca acgccgggaa gaccgtaatt gtggctgcac tggatgggac
      541 cttccagagg aagccatttg gggccatcct gaacctggtg ccgctggccg agagcgtggt
      601 gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc gcctatacca agaggctcgg
      661 cacagaga
//
