LOCUS       Exported                 738 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pWZL Neo Myr Flag VRK3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      35..44
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgatctcct tctgtccaga
      181 ctgtggcaaa agtatccaag cggcattcaa attctgcccc tactgtggaa attctttgcc
      241 tgtagaggag catgtagggt cccagacctt tgtcaatcca catgtgtcat ccttccaagg
      301 ctcaaagaga gggctgaact ccagttttga aacctctcct aagaaagtga aatggtccag
      361 caccgtcacc tctccccgat tatccctctt ctcagatggt gacagttctg agtctgaaga
      421 tactctgagt tcctctgaga gatccaaagg ctccgggagc agacccccaa cccccaaaag
      481 cagccctcag aagaccagga agagccctca ggtgaccagg ggtagccctc agaagaccag
      541 ctgtagccct cagaagacca ggcagagccc tcagacgctg aagcggagcc gagtgaccac
      601 ctcacttgaa gctttgccca cagggacagt gctgacagac aagagtgggc gacagtggaa
      661 gctgaagtcc ttccagacca gggacaacca gggcattctc tatgaagctg cacccacctc
      721 caccctcacc tgtgactc
//
