LOCUS       Exported                3547 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Intersectional viral expression of ChR2(ET/TC)-EYFP in cells 
            expressing Cre AND NOT Flp.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    553..586
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      679..688
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    1124..1157
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     protein_bind    1208..1241
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      1417..1426
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(2048..2081)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     protein_bind    complement(2132..2165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     primer_bind     complement(2387..2406)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2546..2797
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2734..2755
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2807..2840)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    2949..3537
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3002..3022)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3420..3431)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccgaagttcc tattctctag aaagtatagg aacttcgcag agtagtagct
      601 ggattgtagc tgctattagc aatctaaaac ctcttagaag ttcctattct tcaaaaggta
      661 taggaacttc ggcgcgccgc caccatggac tatggcggcg ctttgtctgc cgtcggacgc
      721 gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt ccctgaggat
      781 caatgttact gtgccggatg gattgaatct cgcggcacga acggcgctca gaccgcgtca
      841 aatgtcctgc agtggcttgc agcaggattc agcattttgc tgctgatgtt ctatgcctac
      901 caaacctgga aatctacatg cggctgggag gagatctatg tgtgcgccat tgaaatggtt
      961 aaggtgattc tcgagttctt ttttgagttt aagaatccct ctatgctcta ccttgccaca
     1021 ggacaccggg tgcagtggct gcgctatgca acctggctgc tcacttgtcc tgtcatcctt
     1081 atccacctga gcaacctcac cggtaagtgt cggggttgag ctcataactt cgtatagtat
     1141 accttatacg aagttattct ttgcaccatt ctaaagaata acagtgataa tttctgggtt
     1201 aaggcaaata acttcgtata ggatacttta tacgaagtta tggtacccct cctgtactca
     1261 ccttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     1321 cctcgccgga cacgctgaac ttgtggccgt ttacgtcacc gtccagctcg accaggatgg
     1381 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcggcc gctggcacgg
     1441 ctccggcctc ggcttcgtct tcgacgagag tctcgacctc gatctccgtt ccgccgatgt
     1501 tcagtttggt ggttttgcgg atatctccgt gaatcaatat gtgctcgtgg atcaggacgc
     1561 gcaggtagtg tcccaacaac ccccaacaat ttttactcat cagatcaata atcgtgtgac
     1621 ctacggtgga gccatagacg ctcaggacgc caaaaccttc gggccccaaa atgaagagaa
     1681 ttgggaacat accccagctc acgaaaaaca gccatgccat gccggtcacg acctggcggc
     1741 accgaccctt tggcacagta tgataaccct cgatatatgc tttggcggcg tgaaaaaatg
     1801 tgttcgcgcc atagcacaat ccaagacaaa agaagatgac tttaacatag ccggttgcca
     1861 tggcgctggt agccccccac acgatacacc cgatgtctga gacaaggagt cccatggttc
     1921 tcctgctgta gtcgttgctc aggcctaaac acatgagaaa tagagtcata agcacatggg
     1981 tcacatacag gagatatgta tataacatta atacaatttt ataaaaaaaa gggggggcac
     2041 aactagtata acttcgtata aggtatacta tacgaagtta ttaagaggtt tcatattgct
     2101 aatagcagct acaatccagc taccattctg cataacttcg tataaagtat cctatacgaa
     2161 gttatgtcga ctggagagtg gccagccctt ctcatgttca gagaacatgg ttaactggtt
     2221 aagtcatgtc gtcccacagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     2281 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2341 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2401 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2461 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2521 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2581 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2641 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     2701 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2761 cgccgggatc actctcggca tggacgagct gtacaagtaa gctagcgaag ttcctatact
     2821 ttctagagaa taggaacttc taagcagaca atcttgtaat acatcagcag ctagtagatt
     2881 gctccagcta gaagttccta taccttttga agaataggaa cttcgaattc gatatcaagc
     2941 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3001 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3061 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3121 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3181 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3241 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3301 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3361 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3421 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3481 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     3541 gataccg
//
