LOCUS       Exported                2930 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Viral expression of NpHR3.3-EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-nEF-NpHR3.3-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             1510..1566
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1567..2280
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1609..1630)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1870..1889)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2217..2238
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2332..2920
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2385..2405)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2803..2814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccgccaccat gacagagacc ctgcctcccg tgaccgagag tgccgtggcc
      601 cttcaagccg aggttaccca aagggagttg ttcgagttcg tgctgaacga ccctttgctt
      661 gcaagcagtc tctatatcaa catcgcactt gcaggactga gtatactgct gttcgttttt
      721 atgacccgag gactcgatga tccacgggca aaacttattg ctgtgtcaac catccttgtg
      781 cctgtcgtca gcattgcctc ctacactgga ttggcgagcg gcctgacaat ttccgttctt
      841 gaaatgccag cgggccattt tgcagaaggc agctcagtga tgctgggagg agaagaggta
      901 gatggtgtag tcaccatgtg gggacggtat ctcacctggg cactttccac gcccatgatt
      961 ctcctcgctc tgggtctcct ggccggaagc aatgctacaa agctcttcac agctatcact
     1021 ttcgatatcg ctatgtgcgt gactggcctt gccgcggccc tgactacctc ctcccacctc
     1081 atgagatggt tcttttacgc tatcagttgt gcatgctttc tggtggtctt gtatatcctg
     1141 ctggtggagt gggcacagga cgccaaagcc gcgggaaccg ctgacatgtt caataccctg
     1201 aagctgttga cagtagtgat gtggctgggg tatccaattg tgtgggctct tggagtcgag
     1261 ggtatcgcgg tgttgcccgt tggggtgacg agctggggat attctttcct ggatatcgtg
     1321 gcaaagtaca ttttcgcatt cttgctcctg aactatctga cgtcaaacga atctgtcgtg
     1381 tccggcagca ttttggatgt tccatctgct tctgggaccc cggctgatga taagagcagg
     1441 atcaccagcg agggcgagta catccccctg gaccagatcg acatcaacgt gggcgcgccc
     1501 ggctccggag ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaacccc
     1561 ggtcccgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1621 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1681 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1741 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     1801 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1861 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1921 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1981 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2041 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     2101 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2161 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2221 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2281 ttctgctacg agaacgaggt gtaatgagaa ttcgatatca agcttatcga taatcaacct
     2341 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2401 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2461 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2521 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2581 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2641 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2701 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2761 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2821 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2881 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
//
