LOCUS Exported 10846 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses HA tagged UBE3A in mammalian cells.. ACCESSION . VERSION . KEYWORDS pLVX-HA-UBE3A-IRES-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10846) AUTHORS Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, Brodsky JL, Sobol RW TITLE UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 Ubiquitin Ligase. JOURNAL J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 10.1074/jbc.M116.766824. PUBMED 28003368 REFERENCE 2 (bases 1 to 10846) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10846 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" enhancer 2201..2504 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2505..2708 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2659..2679 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2705..2729 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 2824..2848 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2872..2898 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind complement(5540..5564) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 5596..6169 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5763..5780) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5990..6009 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6202..6801 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6202..6221) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6698..6718 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6815..7403 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6868..6888) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7286..7297) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7610..8243 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8371..8387) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8371..8387) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8384..8406) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8395..8411 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8419..8449) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8464..8485 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8602..8619) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8773..9361) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8853..8872) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9532..10392) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10155..10174 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10393..10497) /gene="bla" /label=AmpR promoter polyA_signal 10545..10679 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10582..10601) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10636..10655 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactaga 2821 tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat gtacccatac 2881 gatgttccag attacgctga gaagctgcac cagtgttatt ggaaatcagg agaacctcag 2941 tctgacgaca ttgaagctag ccgaatgaag cgagcagctg caaagcatct aatagaacgc 3001 tactaccacc agttaactga gggctgtgga aatgaagcct gcacgaatga gttttgtgct 3061 tcctgtccaa cttttcttcg tatggataat aatgcagcag ctattaaagc cctcgagctt 3121 tataagatta atgcaaaact ctgtgatcct catccctcca agaaaggagc aagctcagct 3181 taccttgaga actcgaaagg tgcccccaac aactcctgct ctgagataaa aatgaacaag 3241 aaaggcgcta gaattgattt taaagatgtg acttacttaa cagaagagaa ggtatatgaa 3301 attcttgaat tatgtagaga aagagaggat tattcccctt taatccgtgt tattggaaga 3361 gttttttcta gtgctgaggc attggtacag agcttccgga aagttaaaca acacaccaag 3421 gaagaactga aatctcttca agcaaaagat gaagacaaag atgaagatga aaaggaaaaa 3481 gctgcatgtt ctgctgctgc tatggaagaa gactcagaag catcttcctc aaggataggt 3541 gatagctcac agggagacaa caatttgcaa aaattaggcc ctgatgatgt gtctgtggat 3601 attgatgcca ttagaagggt ctacaccaga ttgctctcta atgaaaaaat tgaaactgcc 3661 tttctcaatg cacttgtata tttgtcacct aacgtggaat gtgacttgac gtatcacaat 3721 gtatactctc gagatcctaa ttatctgaat ttgttcatta tcgtaatgga gaatagaaat 3781 ctccacagtc ctgaatatct ggaaatggct ttgccattat tttgcaaagc gatgagcaag 3841 ctaccccttg cagcccaagg aaaactgatc agactgtggt ctaaatacaa tgcagaccag 3901 attcggagaa tgatggagac atttcagcaa cttattactt ataaagtcat aagcaatgaa 3961 tttaacagtc gaaatctagt gaatgatgat gatgccattg ttgctgcttc gaagtgcttg 4021 aaaatggttt actatgcaaa tgtagtggga ggggaagtgg acacaaatca caatgaagaa 4081 gatgatgaag agcccatccc tgagtccagc gagctgacac ttcaggaact tttgggagaa 4141 gaaagaagaa acaagaaagg tcctcgagtg gaccccctgg aaactgaact tggtgttaaa 4201 accctggatt gtcgaaaacc acttatccct tttgaagagt ttattaatga accactgaat 4261 gaggttctag aaatggataa agattatact tttttcaaag tagaaacaga gaacaaattc 4321 tcttttatga catgtccctt tatattgaat gctgtcacaa agaatttggg attatattat 4381 gacaatagaa ttcgcatgta cagtgaacga agaatcactg ttctctacag cttagttcaa 4441 ggacagcagt tgaatccata tttgagactc aaagttagac gtgaccatat catagatgat 4501 gcacttgtcc ggctagagat gatcgctatg gaaaatcctg cagacttgaa gaagcagttg 4561 tatgtggaat ttgaaggaga acaaggagtt gatgagggag gtgtttccaa agaatttttt 4621 cagctggttg tggaggaaat cttcaatcca gatattggta tgttcacata cgatgaatct 4681 acaaaattgt tttggtttaa tccatcttct tttgaaactg agggtcagtt tactctgatt 4741 ggcatagtac tgggtctggc tatttacaat aactgtatac tggatgtaca ttttcccatg 4801 gttgtctaca ggaagctaat ggggaaaaaa ggaacttttc gtgacttggg agactctcac 4861 ccagttctat atcagagttt aaaagattta ttggagtatg aagggaatgt ggaagatgac 4921 atgatgatca ctttccagat atcacagaca gatctttttg gtaacccaat gatgtatgat 4981 ctaaaggaaa atggtgataa aattccaatt acaaatgaaa acaggaagga atttgtcaat 5041 ctttattctg actacattct caataaatca gtagaaaaac agttcaaggc ttttcggaga 5101 ggttttcata tggtgaccaa tgaatctccc ttaaagtact tattcagacc agaagaaatt 5161 gaattgctta tatgtggaag ccggaatcta gatttccaag cactagaaga aactacagaa 5221 tatgacggtg gctataccag ggactctgtt ctgattaggg agttctggga aatcgttcat 5281 tcatttacag atgaacagaa aagactcttc ttgcagttta caacgggcac agacagagca 5341 cctgtgggag gactaggaaa attaaagatg attatagcca aaaatggccc agacacagaa 5401 aggttaccta catctcatac ttgctttaat gtgcttttac ttccggaata ctcaagcaaa 5461 gaaaaactta aagagagatt gttgaaggcc atcacgtatg ccaaaggatt tggcatgctg 5521 taaaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgatct agttctagag 5581 cggccgcgga tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt 5641 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg 5701 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc 5761 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga 5821 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc 5881 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc 5941 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc 6001 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga 6061 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg 6121 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa 6181 cccacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc 6241 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 6301 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 6361 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 6421 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 6481 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 6541 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 6601 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 6661 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 6721 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 6781 acccgcaagc ccggtgccta gacgcgtctg gaacaatcaa cctctggatt acaaaatttg 6841 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 6901 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 6961 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 7021 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 7081 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 7141 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 7201 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 7261 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 7321 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 7381 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat 7441 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca 7501 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact 7561 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 7621 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 7681 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 7741 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 7801 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 7861 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 7921 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 7981 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 8041 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 8101 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 8161 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 8221 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 8281 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc 8341 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 8401 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 8461 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 8521 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 8581 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 8641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 8701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 8761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 8821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 8881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 8941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 9001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 9061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 9121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 9181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 9241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 9301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 9361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 9421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 9481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 9541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 9601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 9661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 9721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 9781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 9841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 9901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 9961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 10021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 10081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 10141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 10201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 10261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 10321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 10381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 10441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 10501 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag 10561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 10621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca 10681 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca 10741 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat 10801 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //