LOCUS Exported 4026 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC2A1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4026) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4026) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4026 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2151..2250) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2267..2286) /label=T7 /note="T7 promoter, forward primer" promoter complement(2268..2286) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2291..2307) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2291..2307) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2409..2428) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2420..3229 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2487..2506) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3376..3964 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3865..3884 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggagc ctagctccaa gaagctgacc ggccggctga tgctggcagt 721 gggaggagcc gtgctgggca gcctgcagtt cggctacaac accggcgtga tcaatgcccc 781 tcagaaagtg atcgaggagt tttacaatca gacatgggtg caccggtatg gcgagtccat 841 cctgccaacc acactgacca cactgtggtc tctgagcgtg gccatcttct ctgtgggcgg 901 catgatcggc tccttttctg tgggcctgtt cgtgaacaga tttggccgga gaaatagcat 961 gctgatgatg aacctgctgg ccttcgtgtc cgccgtgctg atgggcttct ccaagctggg 1021 caagtctttt gagatgctga tcttggggag attcataatc ggtgtctact gcggcctgac 1081 cacaggcttt gtgcccatgt atgtgggcga ggtgtcccct accgccctga ggggcgccct 1141 gggcacactg caccagctgg gcatcgtggt gggcatcctg atcgcccagg tgttcggcct 1201 ggactccatc atgggcaata aggatctgtg gccactgctg ctgtctatca tctttatccc 1261 cgccctgctg cagtgcatcg tgctgccatt ctgtcccgag agccctcggt ttctgctgat 1321 caacaggaat gaggagaacc gcgccaagtc cgtgctgaag aagctgagag gcaccgccga 1381 cgtgacacac gatctgcagg agatgaagga ggagtctcgg cagatgatga gagagaagaa 1441 ggtgaccatc ctggagctgt tccggagccc agcctacaga cagcccatcc tgatcgccgt 1501 ggtgctgcag ctgagccagc agctgtccgg catcaatgcc gtgttctact atagcacctc 1561 catctttgag aaggccggcg tgcagcagcc agtgtatgcc acaatcggct ctggcatcgt 1621 gaacaccgcc ttcacagtgg tgagcctgtt tgtggtggag agggcaggca ggcgcaccct 1681 gcacctgatc ggactggcag gaatggcagg ctgtgccatc ctgatgacaa tcgccctggc 1741 cctgctggag cagctgccat ggatgtccta cctgtctatc gtggccatct tcggctttgt 1801 ggccttcttt gaagtgggcc ccggccctat cccatggttc atcgtggccg agctgtttag 1861 ccagggacca aggcctgcag caatcgcagt ggccggcttc tctaactgga ccagcaattt 1921 catcgtgggc atgtgctttc agtacgtgga gcagctgtgc ggcccctacg tgttcatcat 1981 ctttaccgtg ctgctggtgc tgttctttat cttcacatac ttcaaggtgc ctgagaccaa 2041 gggcaggaca ttcgacgaga tcgcaagcgg ctttcgccag ggaggagcat ctcagagcga 2101 caagacacct gaggagctgt ttcacccact gggcgccgat tcccaggtgg acccagcttt 2161 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt 2221 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc 2281 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg 2341 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 2401 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa 2461 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 2521 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 2581 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 2641 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 2701 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 2761 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt 2821 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 2881 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 2941 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca 3001 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 3061 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 3121 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 3181 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 3241 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg 3301 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa 3361 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 3421 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 3481 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 3541 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 3601 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 3661 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 3721 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 3781 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 3841 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 3901 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 3961 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 4021 catgtt //