LOCUS Exported 12568 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion and Psd95 shRNA. ACCESSION . VERSION . KEYWORDS FH95pUp95bGW (B5) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12568) AUTHORS Schluter OM, Xu W, Malenka RC TITLE Alternative N-terminal domains of PSD-95 and SAP97 govern activity-dependent regulation of synaptic AMPA receptor function. JOURNAL Neuron. 2006 Jul 6;51(1):99-111. PUBMED 16815335 REFERENCE 2 (bases 1 to 12568) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12568 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 382..580 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 598..778 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 825..950 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1443..1676 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1861..1905 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2203..2320 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2373..2587 /label=H1 promoter /note="human H1 RNA promoter" primer_bind 2508..2527 /label=H1 /note="Human H1 promoter, forward primer" promoter 2691..3902 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3739..3758 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" regulatory 6261..6270 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6267..6986 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6267..6983 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6312..6333) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6573..6592) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6920..6941 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7029..7617 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7082..7102) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7500..7511) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8136..8316 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8342..8359) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8348..8572 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8618..9046 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8705..8724) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8915..8936 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9055..9075) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9060..9389 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9240..9375 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9302..9321 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9437..9484 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9503..9877 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 10007..10128 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10044..10063) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10098..10117 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10177..10193) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10177..10193) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10190..10212) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10201..10217 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10225..10255) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10270..10291 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10408..10425) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10579..11167) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10659..10678) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11338..12198) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11961..11980 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12199..12303) /gene="bla" /label=AmpR promoter primer_bind complement(12378..12397) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg 601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga 841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa 901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc 961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat 1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa 2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg 2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg 2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac 2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag 2341 cagagatcca gtttggttaa tcgtacgaat tcgaacgctg acgtcatcaa cccgctccaa 2401 ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc cctggcagga 2461 agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc 2521 tgggaaatca ccataaacgt gaaatgtctt tggatttggg aatcttataa gttctgtatg 2581 agaccacaga tccccggaca tccaggcaca caagttcaag agacttgtgt gcctggatgt 2641 cctttttgga aagcttatcg aattaattaa cccgtgtcgg ctccagatct ggcctccgcg 2701 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga 2761 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg 2821 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt 2881 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc 2941 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa 3001 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt 3061 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct 3121 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt 3181 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg 3241 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa 3301 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg 3361 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt 3421 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg 3481 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac 3541 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt 3601 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc 3661 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta 3721 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg 3781 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt 3841 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag 3901 acgaagcttg ggctgcaggt cgactctaga ccaccatgtc ccagagacca agagctccca 3961 ggtcagccct ctggctcctg gccccgccac tattgcggtg ggcacctccc ctcctcacag 4021 tgctgcacag tgacctcttc caggccttgc tggacatcct ggactattat gaggcttgca 4081 tcttagagag tcagaaatac cgctaccaag atgaagacac gccccctctg gaacacagcc 4141 cggcccacct ccccaaccag gccaattctc cccctgtgat tgtcaacacg gacaccctag 4201 aagccccagg atatgagttg caggtgaatg gaacagaggg ggagatggag tatgaggaga 4261 tcacattgga aaggggtaac tcaggtctgg gcttcagcat cgcaggtggc actgacaacc 4321 cgcacatcgg tgacgacccg tccattttta tcaccaagat cattcctggt ggggctgcag 4381 cccaggatgg ccgcctcagg gtcaatgaca gcatcctgtt tgtaaatgaa gtggatgttc 4441 gggaggtgac ccattcagct gcggtggagg ccctcaaaga ggcaggttcc atcgttcgcc 4501 tctatgtcat gcgccggaaa cccccagccg aaaaggtcat ggagatcaaa ctcatcaaag 4561 ggcctaaagg acttggcttc agcattgcgg ggggcgttgg gaaccagcac atccctggag 4621 ataacagcat ctatgtaacg aagatcatcg aaggaggtgc tgcccacaag gatggcaggt 4681 tgcagattgg agacaagatc ctggcggtca acagtgtggg gctggaggac gtcatgcacg 4741 aggatgccgt ggcagccctg aagaacacat atgacgttgt gtacctaaag gtggccaagc 4801 ccagcaatgc ctacctgagt gacagctatg ctcccccaga catcacaacc tcgtattctc 4861 agcacctgga caatgagatc agtcatagca gctacttggg cactgactac cccacagcca 4921 tgacccccac ttcccctcgg cgctactccc ctgtggccaa ggacctgctg ggggaggaag 4981 acattccccg ggaaccaagg cggatcgtga tccatcgggg ctccaccggc ctgggcttca 5041 acatcgtggg cggcgaggat ggtgaaggca tcttcatctc cttcatcctt gctgggggtc 5101 cagccgacct cagtggggag ctacggaagg gggaccagat cctgtcggtc aatggtgttg 5161 acctccgcaa tgccagtcac gaacaggctg ccattgccct gaagaatgcg ggtcagacgg 5221 tcacgatcat cgctcagtat aaaccagaag agtatagtcg attcgaggcc aagatccatg 5281 atcttcggga acagctcatg aatagtagcc taggctcagg gactgcatcc ttgcgaagca 5341 accccaagag gggcttctac attagggccc tgtttgatta cgacaagacc aaggactgcg 5401 gtttcttgag ccaggccctg agcttccgct tcggggatgt gcttcatgtc attgacgctg 5461 gtgacgaaga gtggtggcaa gcacggcggg tccactccga cagtgagacc gacgacattg 5521 gcttcattcc cagcaaacgg cgggtcgagc gacgagagtg gtcaaggtta aaggccaagg 5581 actggggctc cagctctgga tcacagggtc gagaagactc ggttctgagc tatgagacgg 5641 tgacccagat ggaagtgcac tatgctcgtc ccatcatcat ccttggaccc accaaagacc 5701 gtgccaacga tgatcttctc tccgagttcc ccgacaagtt tggatcctgt gtccctcata 5761 cgacacgtcc taagcgggaa tatgagatag acggccggga ttaccacttt gtctcctccc 5821 gggagaaaat ggagaaggat attcaagcgc ataagttcat tgaggctggc cagtacaaca 5881 gccacctcta tgggaccagc gtccagtctg tgcgagaggt agcagagcag gggaagcact 5941 gcatcctcga tgtctcggcc aatgccgtgc ggcggctgca ggcggcccac ctgcacccca 6001 tcgccatctt catccgtccc cgctccctgg agaatgtgct agagatcaat aagcggatca 6061 cagaggagca agcccggaaa gccttcgaca gagccacgaa gctggagcag gagttcacag 6121 agtgcttctc agccatcgta gagggcgaca gctttgaaga gatctatcac aaagtgaaac 6181 gtgtcattga agacctctca ggcccctaca tctgggtccc agcccgagag agactcggta 6241 ccgcgggccc gggatccatc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 6301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 6361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 6421 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 6481 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 6541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 6601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 6661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 6721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 6781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 6841 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 6901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 6961 tcggcatgga cgagctgtac aagtaagcta gctcgagcta gaattaattc gatatcaagc 7021 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 7081 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 7141 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 7201 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 7261 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 7321 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 7381 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 7441 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 7501 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 7561 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc 7621 gataccgtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca 7681 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac 7741 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 7801 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc 7861 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag 7921 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga 7981 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg 8041 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc 8101 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct 8161 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 8221 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 8281 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca 8341 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 8401 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 8461 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 8521 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 8581 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 8641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 8701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 8761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 8821 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 8881 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 8941 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 9001 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 9061 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 9121 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 9181 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 9241 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 9301 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 9361 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 9421 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 9481 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 9541 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 9601 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 9661 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 9721 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 9781 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 9841 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc 9901 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 9961 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 10021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 10081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 10141 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 10201 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 10261 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 10321 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 10381 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 10441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 10501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 10561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 10621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 10681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 10741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 10801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 10861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 10921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 10981 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 11041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 11101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 11161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 11221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 11281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 11341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 11401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 11461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 11521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 11581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 11641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 11701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 11761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 11821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 11881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 11941 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 12001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 12061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 12121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 12181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 12241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 12301 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 12361 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc 12421 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg 12481 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt 12541 cgcgatgtac gggccagata tacgcgtt //