LOCUS       Exported                 783 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    T7-Elongin C-pcDNA3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW, 
            Kaelin WG
  TITLE     Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor
            suppressor protein requires binding to complexes containing elongins
            B/C and Cul2.
  JOURNAL   Mol Cell Biol. 1998 Feb . 18(2):732-41.
  PUBMED    9447969
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(439..457)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(440..457)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(477..494)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    483..594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gntagnatga ctggtggana gcaaatggga tccatggatg gagaggagaa aacctatggt
       61 ggctgtgaag gacctgatgc catgtatgtc aaattgatat catctgatgg ccatgaattt
      121 attgtaaaaa gagaacatgc attaacatca ggcacgataa aagccatgtt gagtggccca
      181 ggtcagtttg ctgagaacga aaccaatgag gtcaatttta gagagatacc ttcacatgtg
      241 ctatcgaaag tatgcatgta ttttacgtac aaggttcgct acactaacag ctccaccgag
      301 attcctgaat tcccaattgc acctgaaatt gcactggaac tgctgatggc tgcgaacttc
      361 ttagattgtt aaggtctcca attctgcaga tatccatcac actggcggcc gctcgagcat
      421 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
      481 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      541 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      601 tctgnnnnnt gtcattctat tctggggggg tggggtgnnn nnnggacagc aaggggggag
      661 gattgggaag acaatagcan gcatgctggg gatgcggtgg gctctatggc ttctgangcg
      721 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtannnn nncattnagc
      781 gcg
//
