LOCUS Exported 779 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS HA-Elongin B-pcDNA3.1(+)-Zeo SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 779) AUTHORS Ohh M, Park CW, Ivan M, Hoffman MA, Kim TY, Huang LE, Pavletich N, Chau V, Kaelin WG TITLE Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau protein. JOURNAL Nat Cell Biol. 2000 Jul . 2(7):423-7. PUBMED 10878807 REFERENCE 2 (bases 1 to 779) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..779 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 47..66 /label=T7 /note="T7 promoter, forward primer" promoter 47..65 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 110..136 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" regulatory 149..158 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(580..597) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" gap 634..645 /estimated_length=12 ORIGIN 1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact 61 atagggagac ccaagctggc tagcgtttaa acttaagctt accatggcct acccctacga 121 cgtgcccgac tacgcctccc tcggatccgc caccatggac gtgtttctca tgatccggcg 181 ccacaagacc accatcttca cggacgccaa ggagtccagc acggtgttcg aactgaagcg 241 catcgtcgag ggcatcctca agcggcctcc tgacgagcag cggctgtaca aggatgacca 301 actcttggat gatggcaaga cactgggcga gtgtggcttc accagtcaaa cagcacggcc 361 acaggcccca gccacagtgg ggctggcctt ccgggcagat gacaccttcg aggccctgtg 421 catcgagccg ttttccagcc cgccagagct gcccgatgtg atgaagcccc aggactcggg 481 aagcagtgcc aatgaacaag ctgtgcagtg aattctgcag atatccatca cactggcggc 541 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt 601 gccagccatc tgttgtttgc ccctcccccg tgcnnnnnnn nnnnnggnnn nnnncnnntc 661 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgant angtgtcatt 721 ctattctggg gggtggggtg gggcangaca gcaaggggga ggantgggaa gacaatagc //