LOCUS Exported 4488 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC27A1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4488) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 4488) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4488 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2613..2712) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2729..2748) /label=T7 /note="T7 promoter, forward primer" promoter complement(2730..2748) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2753..2769) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2753..2769) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2871..2890) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2882..3691 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2949..2968) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3838..4426 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4327..4346 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatgaggg cacctggagc aggagcagca tccgtggtgt ctctggccct 721 gctgtggctg ctgggactgc catggacctg gtccgccgca gccgccctgg gcgtgtatgt 781 gggatctgga ggatggaggt tcctgaggat cgtgtgcaag acagcccgga gagatctgtt 841 tggcctgagc gtgctgatca gggtgcgcct ggagctgagg cgccaccaga gggccggcca 901 caccatcccc agaatcttcc aggcagtggt gcagaggcag cctgagagac tggccctggt 961 ggatgcagga accggagagt gttggacatt tgcccagctg gacgcctact ccaacgccgt 1021 ggccaatctg ttccggcagc tgggatttgc accaggcgac gtggtggcca tcttcctgga 1081 gggcagaccc gagtttgtgg gactgtggct gggactggca aaggcaggaa tggaggccgc 1141 cctgctgaac gtgaatctgc ggagagagcc tctggcattc tgcctgggca cctccggagc 1201 aaaggccctg atctttggag gagagatggt ggcagcagtg gcagaggtga gcggacacct 1261 gggcaagtcc ctgatcaagt tctgttctgg cgacctggga cctgagggaa tcctgccaga 1321 cacccacctg ctggatcctc tgctgaagga ggcaagcaca gcaccactgg cacagatccc 1381 ttccaaggga atggacgata ggctgttcta catctatacc agcggaacca caggactgcc 1441 aaaggcagcc atcgtggtgc actcccggta ctatagaatg gccgcctttg gccaccacgc 1501 atataggatg caggcagcag acgtgctgta cgattgcctg cccctgtatc actctgccgg 1561 caacatcatc ggcgtgggcc agtgtctgat ctacggcctg accgtggtgc tgaggaagaa 1621 gttcagcgcc tcccgctttt gggacgattg catcaagtac aattgtacag tggtgcagta 1681 tatcggcgag atctgccggt acctgctgaa gcagccagtg agagaggcag agaggcgcca 1741 ccgggtgaga ctggcagtgg gaaacggact gaggccagca atctgggagg agttcaccga 1801 gaggtttggc gtgcgccaga tcggcgagtt ctatggcgcc acagagtgca actgtagcat 1861 cgccaatatg gatggcaagg tcggctcctg tggctttaac tctaggatcc tgccccacgt 1921 gtaccctatc cgcctggtga aagtgaatga ggataccatg gagctgctga gagacgccca 1981 gggcctgtgc atcccatgtc aggcaggaga gccaggactg ctggtgggac agatcaatca 2041 gcaggacccc ctgcggagat tcgatggcta cgtgagcgag agcgccacat ccaagaagat 2101 cgcccacagc gtgttttcca agggcgattc tgcctacctg agcggcgacg tgctggtcat 2161 ggatgagctg ggctacatgt atttcaggga ccgctctggc gatacctttc ggtggagagg 2221 cgagaacgtg tctaccacag aggtggaggg cgtgctgagc aggctgctgg gacagacaga 2281 cgtggccgtg tacggcgtgg cagtgcctgg agtggagggc aaggcaggca tggccgccgt 2341 ggccgaccct cacagcctgc tggatccaaa tgccatctat caggagctgc agaaggtgct 2401 ggcaccatac gcaaggccca tcttcctgag actgctgcca caggtggaca ccacaggcac 2461 cttcaagatc cagaagacaa ggctgcagag ggagggattt gacccaaggc agacctctga 2521 tcgcctgttc tttctggacc tgaagcaggg ccactatctg cctctgaacg aggccgtgta 2581 cacacgcatc tgcagcggcg cctttgccct ggacccagct ttcttgtaca aagttggcat 2641 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa 2701 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc 2761 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa 2821 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 2881 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga 2941 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 3001 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 3061 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 3121 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 3181 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 3241 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 3301 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 3361 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 3421 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 3481 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 3541 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 3601 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 3661 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg 3721 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt 3781 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 3841 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 3901 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 3961 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 4021 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 4081 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 4141 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 4201 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 4261 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 4321 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 4381 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 4441 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt //