LOCUS Exported 3615 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. ACCESSION . VERSION . KEYWORDS pDONR221_SLC35D1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3615) TITLE RESOLUTE consortium SLC collection REFERENCE 2 (bases 1 to 3615) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3615 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1740..1839) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1856..1875) /label=T7 /note="T7 promoter, forward primer" promoter complement(1857..1875) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1880..1896) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1880..1896) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1998..2017) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2009..2818 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2076..2095) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2965..3553 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3454..3473 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc accatggcag aggtgcaccg gagacagcac gcaagggtga agggagaggc 721 acctgccaag agctccacac tgagggacga ggaggagctg ggaatggcat ctgccgagac 781 actgaccgtg ttcctgaagc tgctggcagc aggcttctac ggcgtgtcta gctttctgat 841 cgtggtggtg aacaagagcg tgctgacaaa ttatcggttt ccatcctctc tgtgcgtggg 901 actgggacag atggtggcaa ccgtggccgt gctgtgggtg ggcaaggccc tgcgggtggt 961 gaagttccca gacctggatc ggaacgtgcc cagaaagacc ttccccctgc ctctgctgta 1021 ctttggcaac cagatcacag gcctgttttc caccaagaag ctgaatctgc ccatgttcac 1081 agtgctgagg cgcttttcta tcctgttcac catgtttgcc gagggcgtgc tgctgaagaa 1141 gacattctcc tggggcatca agatgaccgt gttcgccatg atcatcggag cctttgtggc 1201 agcaagctcc gacctggcct tcgatctgga gggctacgcc tttatcctga tcaacgacgt 1261 gctgaccgcc gccaatggcg cctacgtgaa gcagaagctg gatagcaagg agctgggcaa 1321 gtatggcctg ctgtactata atgccctgtt catgatcctg cctacactgg ccatcgccta 1381 tttcaccggc gacgcccaga aggccgtgga gtttgagggc tgggccgata cactgttcct 1441 gctgcagttt accctgagct gcgtgatggg ctttatcctg atgtacgcca ccgtgctgtg 1501 cacccagtat aactccgccc tgaccacaac catcgtgggc tgtatcaaga atatcctgat 1561 cacctacatc ggcatggtgt tcggcggcga ctatatcttc acatggacca actttatcgg 1621 cctgaatatc tccatcgccg gctctctggt gtacagctat atcaccttca ccgaggagca 1681 gctgtctaag cagagcgagg ccaacaataa gctggatatc aagggcaagg gcgccgtgga 1741 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa 1801 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta 1861 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa 1921 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 1981 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc 2041 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt 2101 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt 2161 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa 2221 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga 2281 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata 2341 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc 2401 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca 2461 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg 2521 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt 2581 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg 2641 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg 2701 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat 2761 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc 2821 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc 2881 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc 2941 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 3001 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 3061 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 3121 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 3181 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 3241 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 3301 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 3361 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 3421 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 3481 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 3541 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 3601 ttttgctcac atgtt //