LOCUS Exported 4454 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses REX-GECO1 in mammalian cells. ACCESSION . VERSION . KEYWORDS CMV-REX-GECO1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4454) AUTHORS Wu J, Abdelfattah AS, Miraucourt LS, Kutsarova E, Ruangkittisakul A, Zhou H, Ballanyi K, Wicks G, Drobizhev M, Rebane A, Ruthazer ES, Campbell RE TITLE A long Stokes shift red fluorescent Ca(2+) indicator protein for two-photon and ratiometric imaging. JOURNAL Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262. PUBMED 25358432 REFERENCE 2 (bases 1 to 4454) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4454 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 697..753 /codon_start=1 /product="calmodulin-binding peptide from avian smooth muscle myosin light chain kinase" /label=calmodulin-binding peptide /translation="RRKWNKAGHAVRAIGRLSS" primer_bind 1449..1468 /label=mCherry-F /note="mCherry, forward primer" promoter complement(1996..2014) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(1997..2014) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2034..2051) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2040..2264 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin complement(2465..3053) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2545..2564) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3224..4084) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3847..3866 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4085..4189) /gene="bla" /label=AmpR promoter primer_bind complement(4264..4283) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt 661 ggtaccgagc tcggatccac catggtcgac tcatcacgtc gtaagtggaa taaggcaggt 721 cacgcagtca gagctatagg tcggctgagc tcacgttggg tttccgagtg gatgtacccc 781 gaggacggcg ccctgaagag cgtgatcaag gaggggttga ggctgaagga cggcggccac 841 tacgccgccg aggtcaggac cacctacaag gccaaaaagc ccgtgcagct gcccggcgcc 901 tacatcgtcg acatcaagtt ggacatcgtg tcccacaacg aggactacac catcgtggaa 961 cagtgcgaac gcgccgaggg ccgccacccc accggcggca tggtcgggct gtacaaggga 1021 ggtacaggcg ggagtctggt gagcaagggc gaggaggata acatggccat catcaaggag 1081 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 1141 ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa ggtgaccaag 1201 ggtggccccc tgcccttcgc ctgggacatc ctgtcccttc agttcatgta cggctccaag 1261 gcctacatta agcacccagc cgacatcccc gactacttca agctgtcctt ccccgagggc 1321 ttcaggtggg agcgcgtgat gatcttcgag gacggcggca ttattcacgt taaccaggac 1381 tcctccctgc aggacggcgt attcatctac aaggtgaagc tgcgcggcac caacttcccc 1441 cccgacggcc ccgtaatgca gaagaagacc atgggctggg agcctacgcg tgaccaactg 1501 actgaagagc agatcgcaga gtttaaagag gctttctccc tatttgacaa ggacggggat 1561 gggacgataa caaccaagga gctggggacg gtgttgcggt ctctggggca gaaccccaca 1621 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacattcgac 1681 ttccctgagt tcctgacgat gatggcaagg aaaatgaatg actcagacag tgaagaggaa 1741 attagagaag cgttccgcgt gtttgataag gacggcaatg gctacatcgg cgcagcagag 1801 cttcgccacg tgatgacaga ccttggagag aagttaacag atgaggaggt tgatgaaatg 1861 atcagggtag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg 1921 atgacagcga agtaggaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca 1981 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac 2041 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 2101 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 2161 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 2221 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 2281 aaccagctgg ggctctagtc cggaactcgc tgcgctcggt cgttcggctg cggcgagcgg 2341 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 2401 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 2461 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 2521 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 2581 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 2641 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 2701 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 2761 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 2821 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 2881 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 2941 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 3001 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 3061 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 3121 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 3181 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 3241 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 3301 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 3361 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 3421 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 3481 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 3541 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 3601 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 3661 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 3721 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 3781 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 3841 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 3901 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 3961 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 4021 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 4081 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 4141 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 4201 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct 4261 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 4321 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 4381 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc 4441 cagatatacg cgtt //