LOCUS Exported 6344 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral control vector containing GFP insert. ACCESSION . VERSION . KEYWORDS pMXspuro-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6344) AUTHORS Kenific CM, Stehbens SJ, Goldsmith J, Leidal AM, Faure N, Ye J, Wittmann T, Debnath J TITLE NBR1 enables autophagy-dependent focal adhesion turnover. JOURNAL J Cell Biol. 2016 Feb 29;212(5):577-590. Epub 2016 Feb 22. PUBMED 26903539 REFERENCE 2 (bases 1 to 6344) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6344 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..375 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 313..329 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" CDS 407..1123 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(452..473) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(713..732) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1060..1081 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(1160..1180) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1165..1494 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1345..1480 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1407..1426 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 1504..2103 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(1504..1523) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 2000..2020 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 2330..2799 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(2929..2946) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3100..3688) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3180..3199) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3852..4712) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4475..4494 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4713..4817) /gene="bla" /label=AmpR promoter LTR 4847..5440 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 5503..5860 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 5919..6335 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 6265..6287 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 6303..6319 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" ORIGIN 1 ccatatgaga tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg 61 acaagagtta ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag 121 cacgaagtct ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta 181 cctcaccctt accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta 241 gaacctcgct ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta 301 gacggcatcg cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga 361 ccatcctcta gactgccgga tctagctagt taattaagga tctaccatgg tgagcaaggg 421 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 481 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 541 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 601 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 661 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 721 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 781 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 841 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 901 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 961 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 1021 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 1081 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtgactcg agcggccgcc 1141 agcacagtgg tcgacctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 1201 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 1261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 1321 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 1381 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 1441 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt 1501 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 1561 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 1621 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 1681 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 1741 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 1801 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 1861 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 1921 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 1981 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 2041 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 2101 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg 2161 tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc 2221 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca 2281 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg 2341 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga 2401 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg 2461 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag 2521 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat 2581 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca 2641 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat 2701 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct 2761 cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt 2821 aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg 2881 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 2941 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 3001 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3061 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3121 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3181 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3241 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3301 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3361 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3421 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3481 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 3541 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3601 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3661 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 3721 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 3781 cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata tgagtaaact 3841 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 3901 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 3961 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 4021 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 4081 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 4141 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 4201 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 4261 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 4321 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 4381 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 4441 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 4501 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 4561 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 4621 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 4681 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 4741 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 4801 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcataatg aaagacccca 4861 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat 4921 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc 4981 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa 5041 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc 5101 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga 5161 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc 5221 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac 5281 aacccctcac tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc 5341 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc 5401 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg 5461 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta 5521 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta 5581 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc 5641 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg 5701 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc cccctaatag gagggatatg 5761 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 5821 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc 5881 tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct 5941 taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag 6001 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg 6061 gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct 6121 tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct 6181 tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc 6241 ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct 6301 ccctttatcc agccctcact ccttctctag gcgcccccat atgg //