LOCUS       Exported                7033 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  To overexpress TIP60 delta HAT in Mammalian Cell.
ACCESSION   .
VERSION     .
KEYWORDS    p3xFLAG-CMV TIP60 delta HAT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             614..679
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    2172..2794
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2371..2390)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2818..2838)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2836..3133
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     3065..3084
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3187..3981
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3241..3260)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3851..3870
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(4510..5098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4590..4609)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5269..6129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5892..5911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6130..6234)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6261..6716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6348..6367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6558..6579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6842..6864
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6856..6873
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6857..6873
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattaacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
      661 caaggatgac gatgacaagc ttgcggccgc aatggcggag gtgggggaga taatcgaggg
      721 ctgccgccta cccgtgctgc ggcggaacca ggacaacgaa gatgagtggc ccctggccga
      781 gatcctgagc gtgaaggaca tcagtggccg gaagcttttc tacgtccatt acattgactt
      841 caacaaacgt ctggatgaat gggtgacgca tgagcggctg gacctaaaga agatccagtt
      901 ccccaagaaa gaggccaaga cccccactaa gaacggactt cctgggtccc gtcctggctc
      961 tccagagaga gaggtgccgg cctcggcgca ggccagcggg aagaccttgc caatcccggt
     1021 ccagatcaca ctccgcttca acctgcccaa ggagcgggag gccattcccg gtggcgagcc
     1081 tgaccagccg ctctcctcca gctcctgcct gcagcccaac caccgctcaa cgaaacggaa
     1141 ggtggaggtg gtttcaccag caactccagt gcccagcgag acagccccgg cctcggtttt
     1201 tccccagaat ggagccgccc gtagggcagt ggcagcccag ccaggacgga agcgaaaatc
     1261 gaattgtttg ggcactgatg aggactccca ggacagctct gatggaatac cgtcagcacc
     1321 acgcatgact ggcagcctgg tgtctgatcg aagccacgac gacatcgtca cccggatgaa
     1381 gaacattgag tgcattgagc tgggccggca ccgcctcaag ccgtggtact tctccccgta
     1441 cccacaggaa ctcaccacat tgcctgtcct ctacctgtgc gagttctgcc tcaagtacgg
     1501 ccgtagtctc aagtgtcttc agcgtcattt gaccaagtgt gacctacgac atcctccagg
     1561 caatgagatt taccgcaagg gcaccatctc cttctttgag attgatggac gtaagaacaa
     1621 gagttattcc cagaacctgt gtcttttggc caagtgtttc cttgaccata agacactgta
     1681 ctatgacaca gaccctttcc tcttctacgt catgacagag tatgactgta agggcttcca
     1741 catcgtgggc tacttctcca aggagaaaga atcaacggaa gactatgaac tctccaaagt
     1801 ggaagggaaa acagggaccc ctgagaagcc cctctcagac cttggcctcc tatcctatcg
     1861 aagctactgg tcccagacca tcctggagat cctgatgggg ctgaagtcgg agagcgggga
     1921 gaggccacag atcaccatca atgagattag tgaaatcacc agcatcaaga aggaggatgt
     1981 catctccact ctgcagtacc tcaatctcat caactactac aagggccagt acatcctcac
     2041 actgtcagag gacatcgtgg atggccatga gcgggccatg ctcaagcggc tcctgcggat
     2101 cgactccaag tgtctgcact tcactcccaa ggactggagc aagaggggga agtggtgatc
     2161 tagaggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     2221 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     2281 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     2341 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     2401 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     2461 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     2521 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     2581 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     2641 ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc
     2701 tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat
     2761 gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga
     2821 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2881 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2941 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3061 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3121 gaggcttttt tggaggaatt gatcagcttg ggatctgatc aagagacagg atgaggatcg
     3181 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3241 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3301 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3361 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3421 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3481 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3541 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3601 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3661 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     3721 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     3781 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     3841 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     3901 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     3961 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4021 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4081 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4141 tcttcgccca ccccgggctc gatcccctcg cgagttggtt cagctgctgc ctgaggctgg
     4201 acgacctcgc ggagttctac cggcagtgca aatccgtcgg catccaggaa accagcagcg
     4261 gctatccgcg catccatgcc cccgaactgc aggagtgggg aggcacgatg gccgctttgg
     4321 tcgacccgga cgggacgctc ctgcgcctga tacagaacga attgcttgca ggcatctcat
     4381 gagtgtgtct tcccgttttc cgcctgaggt cactgcgtgg atggagcgct ggcgcctgct
     4441 gcgcgacggc gagctgctca ccacccactc gccaagctgg aaccgtaaaa aggccgcgtt
     4501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4921 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6241 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6301 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6361 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6421 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6481 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6541 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6601 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6661 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
     6721 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6781 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6841 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgatctatac attgaatcaa
     6901 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     6961 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     7021 tgaccgccat gtt
//
