LOCUS       Exported                5757 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and 
            minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent
            transcriptional activation..
ACCESSION   .
VERSION     .
KEYWORDS    pSynSRE-T-Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Dooley KA, Millinder S, Osborne TF
  TITLE     Sterol regulation of 3-hydroxy-3-methylglutaryl-coenzyme A synthase 
            gene through a direct interaction between sterol regulatory element 
            binding protein and the trimeric CCAAT-binding factor/nuclear factor
            Y.
  JOURNAL   J Biol Chem. 1998 Jan 16;273(3):1349-56.
  PUBMED    9430668
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    438..572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(714..731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(885..1473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(965..984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1644..2504)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2267..2286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2505..2609)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2636..3091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2723..2742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2933..2954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3281..3402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3662..5314
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3728..3746)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          5555..5620
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5750..13
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
       61 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
      121 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
      181 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
      241 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
      301 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
      361 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      421 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      481 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      541 aactcatcaa tgtatcttat catgtctgga tccgtcgacc gatgcccttg agagccttca
      601 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
      661 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
      721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     2101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     2161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     2221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     2401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     2641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     2881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3061 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
     3121 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
     3181 gtaagtaata ttaaggtacg tggaggtttt acttgcttta aaaaacctcc cacacctccc
     3241 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3301 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3361 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatggtactg taactgagct
     3421 aacataaccc gggaggtacc gagctctggc tctcgccgtc tctcgccgtc ccgccattgg
     3481 cctagatgtt ctcgggccac cctacgtcac cgtctcaccc cacttccccg gtcctccgac
     3541 tttgcgctag ctcgacaagg tcaactataa agctgctgag ggcgggaggc acagtgcact
     3601 gtgctccgtg gattgctcct ttctttgccc ccaagcttgg cattccggta ctgttggtaa
     3661 aatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg
     3721 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
     3781 tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc
     3841 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
     3901 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
     3961 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat
     4021 ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca
     4081 aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg
     4141 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
     4201 ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg
     4261 atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc
     4321 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
     4381 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
     4441 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta
     4501 caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct
     4561 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc
     4621 gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata
     4681 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     4741 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     4801 aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg
     4861 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     4921 tggagacata gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc
     4981 tttaattaaa tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca
     5041 acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact
     5101 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     5161 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     5221 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     5281 aaaggccaag aagggcggaa agtccaaatt gtaaaatgta actgtattca gcgatgacga
     5341 aattcttagc tattgtaata ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     5401 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
     5461 aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     5521 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     5581 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     5641 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     5701 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaa
//
