LOCUS Exported 10092 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.. ACCESSION . VERSION . KEYWORDS pAIO-AsCpf1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10092) AUTHORS Nessel T, Beck JM, Rayatpisheh S, Jami-Alahmadi Y, Wohlschlegel JA, Goldberg DE, Beck JR TITLE EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole. JOURNAL Cell Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168. PUBMED 31990132 REFERENCE 2 (bases 1 to 10092) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10092 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(2554..2577) /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS complement(2584..2631) /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS complement(2632..6552) /codon_start=1 /product="CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015)" /label=AsCpf1 /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES KDLKLQNGISNQDWLAYIQELRN" regulatory 2903..2912 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(7654..7673) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7773..7795 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7833..7851) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7919..8023 /gene="bla" /label=AmpR promoter CDS 8024..8884 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8242..8261) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9055..9643 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9544..9563 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9797..9814 /label=L4440 /note="L4440 vector, forward primer" protein_bind 9931..9952 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9967..9997 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10005..10021 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10010..10032 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10029..10045 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10029..10045 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 10063..10081 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 10063..10080 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 gcggccgcaa cggtaaaaat aataacacga attatcacaa taatttttca catatgtata 61 tatatatata tattatcaaa gggacaaccg tttcaagaaa gcaaatgtta ttattttgtt 121 tttattctaa tgtatgtaag ccaaaaacca ataaaaaagt aataattaaa aaggaaataa 181 aataaatata catatgtaat tatacttata taataagtat gtatattttt tattattttt 241 tttttttaat gctatttatt tattattgca tctttttatt taatttttgt atttttttat 301 ataattcctt tatttcttta tttctttctt tatttctttt tttttttttt tttttttttt 361 tttttccttt aattaaactt taaaagacgt tataaatttt aaacatattt tataaataaa 421 aaaaaaaaaa aaaaagacga aataataaaa taaacgaaga taaagtaata ttttgttatt 481 atgtcacata gtatgtgtac tacaattatt tatggatgca aattattaaa ttggtagtta 541 ttatatacat acatgttttt ttttttgtgt gtattaaaag gaactattat ataaatagaa 601 caatttcatg acatttggat ttctacacat cttgaggttt tacaataata tcttcttttt 661 ttttttttct cttctatata tgaaatatat tgtaaaaagg tacaatatat ataatattta 721 tatactatga aaaaaaaaaa atatatacat aaactaataa taatttattc taatttaata 781 taaaaaaaaa ttcttgcttg ttcagataaa taaaagaaat ttatataaat agctatattt 841 tttttttttt tttttttttt tttttctaat tataaatata attattcatt taaaaaattt 901 taaatattta aatatttcat aaattaaaaa aaaaaaaaaa aaaactatta atataataaa 961 cttttatttt tactgtaata taatttttta taatgtaaaa ataaagggta aattattatt 1021 aaaaaatgta tatgttatgt atatataaca taatatatta taatatatat atatatatat 1081 atatatatat aatattagag taaccaaaat gcataatttt tcctatatgc acatatttca 1141 tattaagtat ataatattta atttctactc ttgtagatct taagtttttt attatttcct 1201 ataaaataat atatatatat atatatatat atatatatat atattttaaa tataatgaag 1261 tttttaaggc taccataaaa aaaaaaatac atatatatat aaataaatat attatattat 1321 ataatatata tattaatgtt tttgtttttt aatatttttt tttttttttg taaaatatat 1381 tatttttgga cttataaata attgtgtaca tacatatatt attttattat aataaatata 1441 ataatactat aataatcttt atgcaatatt tgtaattcat atataataat ttttaaaatt 1501 atctatcttt tgaaaaaaaa aaaaaaataa aaataataaa aattataatg ttattaaata 1561 ctataattat tatgattaat ataatatata tatatatata tatatatata tatatatata 1621 tatattttat atcatttaat atattatgtg tgatataata ataaatccat tgtccataaa 1681 tagatgaaat agcggccgcc taccctgaag aagaaaagtc cgatgatgtt gtggacataa 1741 agaattgagc tgtctcaaaa aatgagaaaa caaacaaaca aacaaaatac cattatgcgc 1801 atatcctctg ctcaatatca atttttcttt atgccagttt tgtttcgaca ctatttgagc 1861 aacaccttcc ctttactatc tactactgtt acaatgttat tttattcacc tttttacaat 1921 tttatcaaaa tttttttgta tgcatataaa ttatacattg taaagccaaa gtgtagataa 1981 agacaccttg tttatttttc cccaattttt tttttttacc atttttttat taaccttgct 2041 catttacctg ctaatacgat tgctatatga tatcgaaatt gaaggaaaaa ccatcatttg 2101 tgtttatatg ttttttttta atttttcaac tttttttatt taactatgct aatcatatta 2161 taaacatttt aaaaatcaag tccaactatt tatgaatcat tgaaggagac acataattat 2221 aacagtaaaa ataataataa taataataat tttgtcttat tgtttcaaac atattatgtt 2281 ccataaatat ctaaattttt aatatggtca taaattcata ggatttaatt tatataaaat 2341 atggctattc atactagcca ttttatgtgt gtaaagtttt ttttttctga acaaaaaata 2401 aaatgttcat aattttagct atttacatgc atgtgcatgc acaaaaaaaa atatgcacac 2461 aacatacaca tttttacagt tataaataca atcaattggt ataaatatat aaataagaaa 2521 aacgaacatt aagctgccat atccctcgag ttacttgtcg tcgtcgtcct tgtagtcgga 2581 tccctttttc ttttttgcct ggccggcctt tttcgtggcc gccggccttt tgttgcgcag 2641 ctcctggatg taggccagcc agtcctgatt ggagatgccg ttctgcagct tcagatcctt 2701 gctctccttc aggtgattca gcagcagctg gcccttcagg gcgatgtggt aggcgccatt 2761 ggcatcggcg tccatgggcc actctgggtt ctgaaaccgg gagtcgaagc acacgccatt 2821 cagatcgcgc acggggctgt tgatatagtc ctcgcctgtg gcggcattgg agttccgcat 2881 ctgcagcacg ctgcggatca gggccaccat ggtgtcgatg gcgtgagaat cgtcattctc 2941 cagcagcttt ggcaggatgt tggagccatc cctgaacacg atgcccttct cctccagcag 3001 ggcgatcagc tcgttggcag gatacaggtc ccggtatctg ccggtgaatc tgtgattctc 3061 gatcactggc acgattctct tgccggcgat gaaaggggtg cccttggcgt caaactgtgt 3121 ctcgttcttc tcgaacacga tatcccatgc aggcataaag ccgggcaggc ccctctggaa 3181 ggacagattt ctgttcatct taaagtgcag gatgaagtcg ccggttttca cgtcgtagtg 3241 cagaaagtcg aagccctcca ggaagtgctt gcggctctcg tgattcttga tggttttcca 3301 cacgaagggg tccacgaagc cggtcagggg atcgatctta gatgtatatg gggcaggcac 3361 gtaaaacagg aagccagact gggtgcccat cttggcaaag gaggtgaact ggtctgtcag 3421 ctggtatggg ttcagcacgc ctcccacttt ctctgctgga tagtccttca gcaccaggca 3481 attcagctta tcgatcagca tcttctcgaa ctgctggtac acggccttct cggcgatgcc 3541 ggtcctcttg ctcttaaagc cgaaattcag gttctccagc accaccacgg cctggtagtg 3601 gatcatcagg tccacgatct cgtggatgac ctggctcaga tagccctgct tcagatcctt 3661 gattgtgccc accacagacc aggcctgcct tgctgccacc ctctccttct ccctgttgtc 3721 cagcttcttc tggtaatcaa actgctggat ggtgttcagg ctccgctgct ccaggatctt 3781 gccggtggag tcgatcactg tgatatagat caggtttctc tcgccccgat cgatgccgat 3841 gataggtgtc tcggggtgct ccttcaggta ggcattcacc ctctggttga acttagatgg 3901 ggaattggcg gcctgatagt tcagtgtgat aggcacgtgg aaaaagaact tgtcgctggt 3961 aaagcgccta tccttgatga tctcgtgaga cacctccttg gtgatcacgt tgggcagcag 4021 ggccctggcc tcatcagaca ggtcgtggga cagtctgtga ttcacatagt cgtacagctc 4081 ctggtacagg gtgtcgggga ttggggtttt ctgatccttc agcttcttgt tcagcatctt 4141 ctctcccagc cggtgtgcca tcctcttcat cctggactta gggcggtaga acagctcggc 4201 ctggccattc agcttgatgc ttgtcttggc caggttctct ggagaaaaca ggccggtcca 4261 atacagtgtg tgcagattag gcttgccgtg gtggcccttg gcaaagtcct tgttatagat 4321 ctggaacagg tacagcttgc ctgtctccac ggcatccatg atctccttct cggcgattct 4381 ctggaagctg atgtggtaca gcaggggatt cagctcggca tagtactcgc ccaggtcctt 4441 atactgagag gatggccgca ggctagacag atcgatagag gttgtcttgg tatacttgga 4501 cagaaaatcc cttgtgaagt cgatccactt gcacagggcc tctctgtagc ccttctggtc 4561 gccggttttc ttggcgtagg ctgtctgaaa cttctttggc tccttctcag gattgttcag 4621 gtcgtagatc tcctttgtga tctccagagg ctcgatgaaa ttgttggaca gcaggatggg 4681 ggttgtgtgg gtctgaaagt gggctgtcac ggccttcagc tgggtgctgc actttgggat 4741 catcttggcg gcatcaggga agtagtcata gtacatctta tcaaagccct cgctggtttt 4801 ctctgtgggc tcgaagctca gggccttata cctgcccttc tgctttggca tgatgcccag 4861 atagtacagg ccgttcttca caaacaggat ggcgccattg ttcttctcct tattcacgtc 4921 ccagccagag gccagtgtag gcatctgaaa gttcagcttg aacttctcca cggagtaggg 4981 cttcttggtg gcataatttc tggccttgtt gtagaagctc agagaaggct ccatctccag 5041 cttgatgccg gtcagccggg cagagaactc ggggtccacc tcgttggact catccacggc 5101 aaaccagtcc agcaggtggt acaggcccag caggctgtcc agctgagact tcaggatctc 5161 cttctcctcc tgcttcttca gggttgtagg cagtggctga tccagggcgg cgtgtgcgtg 5221 ggacaggatc tcgctggttt tctgcttgaa ggcctcgctc agctccttgc ctgcggcaga 5281 gatgatctcc tgcaggttga tatcctcgtg cttcaggctg cgctgcacct tctccttggc 5341 agacttggtg atcttgcctg tcagctcgga gattctccgc tcatacaggg cattcctcag 5401 tgtatcccag tggtcgcaca gggcgctgct gattgtctcc agcttcttgt ggctgatgaa 5461 gatgtgtgtc aggtcgatgc tgttcagctc gttaaacagg gcctcggctg tctccagcac 5521 gttctcgttt ctcagcagtg tcttgtactt gcagaaggac tggatcactt cctcgtcgct 5581 cttaaactcc tccaggatga aagacagggt gttcctatcg gacaggatct gcttaaacag 5641 ggggatgaat ctgtgtggca gggaggcgat gatgtgggct gtctcatcat tcttctggat 5701 ggccagattc agcacctcgt tcaggccctt gatcttctcg gtgcctgcct cccgagagat 5761 tcctcccagc agctggttat acaggtcgat ctgggtctgt gtcagcagct ggttataaaa 5821 agggaaggaa aacacctcct cgatggaggt gctcacgaag atgccgatgg ccttcttcac 5881 gttctcaaag tgctcccgca ggctgggcac ggcggtgatc aggcgtgtga agatgtgaca 5941 attctcctta aacttgggga agttgtcctg cacgatgcgg tgtgggatgg ctgtgctgat 6001 atcctcggcg ctgaacacgt tcttcctgtt ctcataaaag ccggagaagt aggttgtaaa 6061 cttgtcgaag ctccgcagca gggcgttctc gtgctcggtt gtggtcacgg tgcccagctg 6121 cttcagcacc ttgccattaa acagctcggc cttgaacagg cccttgtaga tctcggcgtg 6181 tctcttattg atggcatcgg tcaggttgtc tgtccggccg atgaagtagt cgtggatggc 6241 attgcgatat gtggcctgct cctcgatcag ggcgttcctt gtctcctcgg ttttctcctt 6301 tctataggag tcgatggcgg cgctcaggtt ctcccaatcc agctgcacca gctgcaggca 6361 ctggtcggca taggtcttgt agatccgatc gatgatgggc ttcagctcct tgtagtgatc 6421 attgcgggcc ttgtcctcct cgatgaagcc ctgctcctgg atgtgcttca gggtcttgcc 6481 ctgtgggatc agctcaaacc gcagtgtctt gctcacctga tacaggttgg taaagccctc 6541 gaactgtgtc atggatcctg atatatttct attaggtatt tattattata aaatataaat 6601 cttgaatgat aataaataaa atattagtta ttccttttct agtttaaaat atacatatta 6661 taaatatata tatatatata tatattttta ttgtgacaag aatatataat tataaattat 6721 attatttatt tttgtatttt tttttttttt tttttttttt tctttttttg ttttattttt 6781 cttttttttt ataaatatta tttttttctt ttatcatgca cattggaata atacattaat 6841 atatatatat atattatatt atacatatat tgaataatgt ttataaaaaa tgcataactt 6901 atatgaatat aatttttttt aaatatgaca aaaagaaaaa aaaaaaaaac caaaaaaaat 6961 taaaattgaa atgaaatata taaatatatt atttatatat attatacatt gtttaatact 7021 actacatgta tatatatata ttatatatat atatatatat caattttttc aaaaataaat 7081 taatataaaa agaggggaaa aaaaaaaaaa aaaaaaaaaa aagataatta agtaagcatt 7141 taaaaatata taaattgata atatataaaa ttaatcacat ataaactaat ataatttata 7201 aaataaggaa aataaaatat taccataaaa taaaaataaa aataaaaaaa aaaaaaaaaa 7261 acaccttttt ttatatatat taatatataa ttatctctta gaaaaaatat tgtataatta 7321 tatatgtaat gatttatata aaaaaataaa attatacaag tatatatttt gtttctataa 7381 attgatatct taattattta ttattagaaa tagatatttt tataataaac caatagataa 7441 aatttgtaga gaaaaaaaaa taaaaataaa aataaaaata atataatata taataaaata 7501 aaataatatt atataaatat attttaattt tttttacaaa atgtctagaa ttcatatcga 7561 taactccatg gaactcctag gctggcgcct gatgcggtat tttctcctta cgcatctgtg 7621 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 7681 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 7741 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 7801 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 7861 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 7921 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 7981 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 8041 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 8101 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 8161 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 8221 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 8281 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 8341 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 8401 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 8461 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 8521 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatgccaac 8581 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 8641 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 8701 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 8761 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 8821 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 8881 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 8941 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 9001 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 9061 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 9121 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 9181 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 9241 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 9301 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 9361 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 9421 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 9481 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 9541 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 9601 tcgatttttg tgatgctcgt caggggggcg gagcctatcg aaaaacgcca gcaacgcggc 9661 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 9721 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 9781 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 9841 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 9901 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 9961 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 10021 atttcacaca ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag 10081 aatactcaag ct //